Caffeine inhibits homology-directed repair of I-SceI-induced DNA double-strand breaks

Huichen Wang, Wilfried Boecker, Hongyan Wang, Xiang Wang, Jun Guan, Larry H. Thompson, Jac A. Nickoloff, George Iliakis

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

We recently reported that two Chinese hamster mutants deficient in the RAD51 paralogs XRCC2 and XRCC3 show reduced radiosensitization after treatment with caffeine, thus implicating homology-directed repair (HDR) of DNA double-strand breaks (DSBs) in the mechanism of caffeine radiosensitization. Here, we investigate directly the effect of caffeine on HDR initiated by DSBs induced by a rare cutting endonuclease (I-SceI) into one of two direct DNA repeats. The results demonstrate a strong inhibition by caffeine of HDR in wild-type cells, and a substantial reduction of this effect in HDR-deficient XRCC3 mutant cells. Inhibition of HDR and cell radiosensitization to killing shows similar dependence on caffeine concentration suggesting a cause-effect relationship between these effects. UCN-01, a kinase inhibitor that effectively abrogates checkpoint activation in irradiated cells, has only a small effect on HDR, indicating that similar to radiosensitization, inhibition of checkpoint signaling is not sufficient for HDR inhibition. Recombination events occurring during treatment with caffeine are characterized by rearrangements reminiscent to those previously reported for the XRCC3 mutant, and immunofluorescence microscopy demonstrates significantly reduced formation of IR-specific RAD51 foci after caffeine treatment. In summary, our results identify inhibition of HDR as a significant contributor to caffeine radiosensitization.

Original languageEnglish (US)
Pages (from-to)824-834
Number of pages11
JournalOncogene
Volume23
Issue number3
DOIs
StatePublished - Jan 22 2004
Externally publishedYes

Fingerprint

Double-Stranded DNA Breaks
Caffeine
Nucleic Acid Repetitive Sequences
Deoxyribonuclease I
Cricetulus
Fluorescence Microscopy
Genetic Recombination
Phosphotransferases
DNA

Keywords

  • Caffeine
  • DNA
  • Double-strand breaks (DSB)
  • Homologous recombination
  • Homology-directed repair
  • Nonhomologous end joining
  • Repair

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Wang, H., Boecker, W., Wang, H., Wang, X., Guan, J., Thompson, L. H., ... Iliakis, G. (2004). Caffeine inhibits homology-directed repair of I-SceI-induced DNA double-strand breaks. Oncogene, 23(3), 824-834. https://doi.org/10.1038/sj.onc.1207168

Caffeine inhibits homology-directed repair of I-SceI-induced DNA double-strand breaks. / Wang, Huichen; Boecker, Wilfried; Wang, Hongyan; Wang, Xiang; Guan, Jun; Thompson, Larry H.; Nickoloff, Jac A.; Iliakis, George.

In: Oncogene, Vol. 23, No. 3, 22.01.2004, p. 824-834.

Research output: Contribution to journalArticle

Wang, H, Boecker, W, Wang, H, Wang, X, Guan, J, Thompson, LH, Nickoloff, JA & Iliakis, G 2004, 'Caffeine inhibits homology-directed repair of I-SceI-induced DNA double-strand breaks', Oncogene, vol. 23, no. 3, pp. 824-834. https://doi.org/10.1038/sj.onc.1207168
Wang H, Boecker W, Wang H, Wang X, Guan J, Thompson LH et al. Caffeine inhibits homology-directed repair of I-SceI-induced DNA double-strand breaks. Oncogene. 2004 Jan 22;23(3):824-834. https://doi.org/10.1038/sj.onc.1207168
Wang, Huichen ; Boecker, Wilfried ; Wang, Hongyan ; Wang, Xiang ; Guan, Jun ; Thompson, Larry H. ; Nickoloff, Jac A. ; Iliakis, George. / Caffeine inhibits homology-directed repair of I-SceI-induced DNA double-strand breaks. In: Oncogene. 2004 ; Vol. 23, No. 3. pp. 824-834.
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