Caenorhabditis elegans DYF-2, an orthologue of human WDR19, is a component of the intraflagellar transport machinery in sensory cilia

Evgeni Efimenko, Oliver E. Blacque, Guangshuo Ou, Courtney J. Haycraft, Bradley K. Yoder, Jonathan M. Scholey, Michel R. Leroux, Peter Swoboda

Research output: Contribution to journalArticle

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Abstract

The intraflagellar transport (IFT) machinery required to build functional cilia consists of a multisubunit complex whose molecular composition, organization, and function are poorly understood. Here, we describe a novel tryptophan-aspartic acid (WD) repeat (WDR) containing IFT protein from Caenorhabditis elegans, DYF-2, that plays a critical role in maintaining the structural and functional integrity of the IFT machinery. We determined the identity of the dyf-2 gene by transgenic rescue of mutant phenotypes and by sequencing of mutant alleles. Loss of DYF-2 function selectively affects the assembly and motility of different IFT components and leads to defects in cilia structure and chemosensation in the nematode. Based on these observations, and the analysis of DYF-2 movement in a Bardet-Biedl syndrome mutant with partially disrupted IFT particles, we conclude that DYF-2 can associate with IFT particle complex B. At the same time, mutations in dyf-2 can interfere with the function of complex A components, suggesting an important role of this protein in the assembly of the IFT particle as a whole. Importantly, the mouse orthologue of DYF-2, WDR19, also localizes to cilia, pointing to an important evolutionarily conserved role for this WDR protein in cilia development and function.

Original languageEnglish (US)
Pages (from-to)4801-4811
Number of pages11
JournalMolecular Biology of the Cell
Volume17
Issue number11
DOIs
StatePublished - Nov 2006

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Cilia
Caenorhabditis elegans
Bardet-Biedl Syndrome
Aspartic Acid
Tryptophan
Carrier Proteins
Proteins
Alleles
Phenotype
Mutation
Genes
WD40 Repeats

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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Caenorhabditis elegans DYF-2, an orthologue of human WDR19, is a component of the intraflagellar transport machinery in sensory cilia. / Efimenko, Evgeni; Blacque, Oliver E.; Ou, Guangshuo; Haycraft, Courtney J.; Yoder, Bradley K.; Scholey, Jonathan M.; Leroux, Michel R.; Swoboda, Peter.

In: Molecular Biology of the Cell, Vol. 17, No. 11, 11.2006, p. 4801-4811.

Research output: Contribution to journalArticle

Efimenko, E, Blacque, OE, Ou, G, Haycraft, CJ, Yoder, BK, Scholey, JM, Leroux, MR & Swoboda, P 2006, 'Caenorhabditis elegans DYF-2, an orthologue of human WDR19, is a component of the intraflagellar transport machinery in sensory cilia', Molecular Biology of the Cell, vol. 17, no. 11, pp. 4801-4811. https://doi.org/10.1091/mbc.E06-04-0260
Efimenko, Evgeni ; Blacque, Oliver E. ; Ou, Guangshuo ; Haycraft, Courtney J. ; Yoder, Bradley K. ; Scholey, Jonathan M. ; Leroux, Michel R. ; Swoboda, Peter. / Caenorhabditis elegans DYF-2, an orthologue of human WDR19, is a component of the intraflagellar transport machinery in sensory cilia. In: Molecular Biology of the Cell. 2006 ; Vol. 17, No. 11. pp. 4801-4811.
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