Ca sparks do not explain all ryanodine receptor-mediated SR Ca leak in mouse ventricular myocytes

Demetrio J. Santiago, Jerald W. Curran, Donald M Bers, W. J. Lederer, Michael D. Stern, Eduardo Ríos, Thomas R. Shannon

Research output: Contribution to journalArticle

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Abstract

Diastolic Ca leak from the sarcoplasmic reticulum (SR) of ventricular myocytes reduces the SR Ca content, stabilizing the activity of the SR Ca release channel ryanodine receptor for the next beat. SR Ca leak has been visualized globally using whole-cell fluorescence, or locally using confocal microscopy, but never both ways. When using confocal microscopy, leak is imaged as "Ca sparks," which are fluorescent objects generated by the local reaction-diffusion of released Ca and cytosolic indicator. Here, we used confocal microscopy and simultaneously measured the global ryanodine-receptor- mediated leak rate (Jleak) and Ca sparks in intact mouse ventricular myocytes. We found that spark frequency and Jleak are correlated, as expected If both are manifestations of a common phenomenon. However, we also found that sparks explain approximately half of Jleak. Our strategy unmasks the presence of a subresolution (i.e., nonspark) release of potential physiological relevance.

Original languageEnglish (US)
Pages (from-to)2111-2120
Number of pages10
JournalBiophysical Journal
Volume98
Issue number10
DOIs
StatePublished - May 19 2010

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Ryanodine Receptor Calcium Release Channel
Sarcoplasmic Reticulum
Muscle Cells
Confocal Microscopy
Fluorescence

ASJC Scopus subject areas

  • Biophysics

Cite this

Santiago, D. J., Curran, J. W., Bers, D. M., Lederer, W. J., Stern, M. D., Ríos, E., & Shannon, T. R. (2010). Ca sparks do not explain all ryanodine receptor-mediated SR Ca leak in mouse ventricular myocytes. Biophysical Journal, 98(10), 2111-2120. https://doi.org/10.1016/j.bpj.2010.01.042

Ca sparks do not explain all ryanodine receptor-mediated SR Ca leak in mouse ventricular myocytes. / Santiago, Demetrio J.; Curran, Jerald W.; Bers, Donald M; Lederer, W. J.; Stern, Michael D.; Ríos, Eduardo; Shannon, Thomas R.

In: Biophysical Journal, Vol. 98, No. 10, 19.05.2010, p. 2111-2120.

Research output: Contribution to journalArticle

Santiago, DJ, Curran, JW, Bers, DM, Lederer, WJ, Stern, MD, Ríos, E & Shannon, TR 2010, 'Ca sparks do not explain all ryanodine receptor-mediated SR Ca leak in mouse ventricular myocytes', Biophysical Journal, vol. 98, no. 10, pp. 2111-2120. https://doi.org/10.1016/j.bpj.2010.01.042
Santiago, Demetrio J. ; Curran, Jerald W. ; Bers, Donald M ; Lederer, W. J. ; Stern, Michael D. ; Ríos, Eduardo ; Shannon, Thomas R. / Ca sparks do not explain all ryanodine receptor-mediated SR Ca leak in mouse ventricular myocytes. In: Biophysical Journal. 2010 ; Vol. 98, No. 10. pp. 2111-2120.
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