Abstract
Immunofluorescence detection of the complement split product C4d along peritubular capillaries in renal allograft biopsies is the mainstay for the diagnosis of antibody-mediated rejection. The extent of peritubular capillary C4d positivity may have significant clinical ramifications; however, peritubular capillary density in the renal cortex is often difficult to assess with single-channel immunofluorescence. In this study, we report a C4d/CD34 double-immunofluorescence staining protocol for renal allograft frozen sections that allows rapid and sensitive detection of C4d positivity, as well as improved accuracy in estimating the C4d-positive fraction of peritubular capillaries. In addition, this method aids in determining whether C4d-positive structures correspond to peritubular capillaries or whether they represent common mimics of peritubular capillaries such as tubular basement membranes. C4d/CD34 double immunofluorescence provides rapid, convenient, and low-cost implementation for laboratories currently utilizing single-channel C4d immunofluorescence.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 434-438 |
| Number of pages | 5 |
| Journal | Modern Pathology |
| Volume | 25 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 1 2012 |
| Externally published | Yes |
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Keywords
- antibody mediated
- C4d
- CD34
- immunofluorescence
- peritubular capillaries
- rejection
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
C4d/CD34 double-immunofluorescence staining of renal allograft biopsies for assessing peritubular capillary C4d positivity. / Jen, Kuang-Yu; Nguyen, Thuy B.; Vincenti, Flavio G.; Laszik, Zoltan G.
In: Modern Pathology, Vol. 25, No. 3, 01.03.2012, p. 434-438.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - C4d/CD34 double-immunofluorescence staining of renal allograft biopsies for assessing peritubular capillary C4d positivity
AU - Jen, Kuang-Yu
AU - Nguyen, Thuy B.
AU - Vincenti, Flavio G.
AU - Laszik, Zoltan G.
PY - 2012/3/1
Y1 - 2012/3/1
N2 - Immunofluorescence detection of the complement split product C4d along peritubular capillaries in renal allograft biopsies is the mainstay for the diagnosis of antibody-mediated rejection. The extent of peritubular capillary C4d positivity may have significant clinical ramifications; however, peritubular capillary density in the renal cortex is often difficult to assess with single-channel immunofluorescence. In this study, we report a C4d/CD34 double-immunofluorescence staining protocol for renal allograft frozen sections that allows rapid and sensitive detection of C4d positivity, as well as improved accuracy in estimating the C4d-positive fraction of peritubular capillaries. In addition, this method aids in determining whether C4d-positive structures correspond to peritubular capillaries or whether they represent common mimics of peritubular capillaries such as tubular basement membranes. C4d/CD34 double immunofluorescence provides rapid, convenient, and low-cost implementation for laboratories currently utilizing single-channel C4d immunofluorescence.
AB - Immunofluorescence detection of the complement split product C4d along peritubular capillaries in renal allograft biopsies is the mainstay for the diagnosis of antibody-mediated rejection. The extent of peritubular capillary C4d positivity may have significant clinical ramifications; however, peritubular capillary density in the renal cortex is often difficult to assess with single-channel immunofluorescence. In this study, we report a C4d/CD34 double-immunofluorescence staining protocol for renal allograft frozen sections that allows rapid and sensitive detection of C4d positivity, as well as improved accuracy in estimating the C4d-positive fraction of peritubular capillaries. In addition, this method aids in determining whether C4d-positive structures correspond to peritubular capillaries or whether they represent common mimics of peritubular capillaries such as tubular basement membranes. C4d/CD34 double immunofluorescence provides rapid, convenient, and low-cost implementation for laboratories currently utilizing single-channel C4d immunofluorescence.
KW - antibody mediated
KW - C4d
KW - CD34
KW - immunofluorescence
KW - peritubular capillaries
KW - rejection
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UR - http://www.scopus.com/inward/citedby.url?scp=84857787989&partnerID=8YFLogxK
U2 - 10.1038/modpathol.2011.168
DO - 10.1038/modpathol.2011.168
M3 - Article
C2 - 22037262
AN - SCOPUS:84857787989
VL - 25
SP - 434
EP - 438
JO - Modern Pathology
JF - Modern Pathology
SN - 0893-3952
IS - 3
ER -