C-type natriuretic peptide system in fetal ovine pulmonary vasculature

Satyanarayana Lakshminrusimha, Christopher A. D'Angelis, James A. Russell, Lori C. Nielsen, Sylvia F. Gugino, Peter A. Nickerson, Robin H Steinhorn

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

C-type natriuretic peptide (CNP) is a recently described endothelium-derived relaxing factor. CNP relaxes vascular smooth muscle and inhibits smooth muscle proliferation by binding to natriuretic peptide receptor (NPR) type B (NPR-B) and producing cGMP. Lung parenchyma and fifth-generation pulmonary arteries (PA) and veins (PV) were isolated from late-gestation fetal lambs. All three types of NPR mRNA were detected in PA and PV by RT-PCR. CNP and NPR-B immunostaining was positive in pulmonary vascular endothelium and medial smooth muscle. CNP concentration-response curves of PA and PV were compared with those of atrial natriuretic peptide (ANP) by use of standard tissue bath techniques. CNP relaxed PV significantly better than PA. ANP relaxed PA and PV equally, but ANP relaxed PA significantly better than CNP. Pretreating PA and PV with natriuretic peptide receptor blocker (HS-142-1) or cGMP-dependent protein kinase inhibitor Rp-β-phenyl-1-N2-etheno-8-bromoguanosine 3′,5′-cyclic monophosphorothionate significantly inhibited the CNP relaxation response, indicating that the response was mediated through the NPR-cGMP pathway. We conclude that CNP is important in mediating pulmonary venous tone in the fetus.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume281
Issue number2 25-2
StatePublished - 2001
Externally publishedYes

Fingerprint

C-Type Natriuretic Peptide
Sheep
Pulmonary Artery
Pulmonary Veins
Lung
Natriuretic Peptides
Peptide Receptors
Atrial Natriuretic Factor
Smooth Muscle
Cyclic GMP-Dependent Protein Kinases
Endothelium-Dependent Relaxing Factors
Brain Natriuretic Peptide
Vascular Endothelium
Protein Kinase Inhibitors
Baths
Vascular Smooth Muscle
Veins
Fetus
Pregnancy
Polymerase Chain Reaction

Keywords

  • Guanosine 3′,5′-cyclic monophosphate
  • Natriuretic peptide receptors
  • Protein kinase G

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Lakshminrusimha, S., D'Angelis, C. A., Russell, J. A., Nielsen, L. C., Gugino, S. F., Nickerson, P. A., & Steinhorn, R. H. (2001). C-type natriuretic peptide system in fetal ovine pulmonary vasculature. American Journal of Physiology - Lung Cellular and Molecular Physiology, 281(2 25-2).

C-type natriuretic peptide system in fetal ovine pulmonary vasculature. / Lakshminrusimha, Satyanarayana; D'Angelis, Christopher A.; Russell, James A.; Nielsen, Lori C.; Gugino, Sylvia F.; Nickerson, Peter A.; Steinhorn, Robin H.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 281, No. 2 25-2, 2001.

Research output: Contribution to journalArticle

Lakshminrusimha, S, D'Angelis, CA, Russell, JA, Nielsen, LC, Gugino, SF, Nickerson, PA & Steinhorn, RH 2001, 'C-type natriuretic peptide system in fetal ovine pulmonary vasculature', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 281, no. 2 25-2.
Lakshminrusimha, Satyanarayana ; D'Angelis, Christopher A. ; Russell, James A. ; Nielsen, Lori C. ; Gugino, Sylvia F. ; Nickerson, Peter A. ; Steinhorn, Robin H. / C-type natriuretic peptide system in fetal ovine pulmonary vasculature. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2001 ; Vol. 281, No. 2 25-2.
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