Abstract
OBJECTIVE - C-Reactive protein (CRP), a cardiovascular risk marker, could also participate in atherosclerosis. Atherosclerotic plaques express CRP and interleukin (IL)-10, a major antiinflammatory cytokine. IL-10 deficiency results in increased lesion formation, whereas IL-10 delivery attenuates lesions. We tested the effect of CRP on lipopolysaccharide (LPS)-induced IL-10 secretion in human monocyte-derived macrophages (HMDMs). METHODS AND RESULTS - Incubation of HMDMs with CRP significantly decreased LPS-induced IL-10 mRNA and intracellular and secreted IL-10 protein and destabilized IL-10 mRNA. Also, CRP alone increased secretion of IL-8, IL-6, and tumor necrosis factor from HMDMs and did not inhibit LPS-induced secretion of these cytokines. Fc γ receptor I antibodies significantly reversed CRP-mediated IL-10 inhibition. CRP significantly decreased intracellular cAMP, phospho-cAMP response element binding protein (pCREB), and adenyl cyclase activity. cAMP agonists reversed CRP-mediated IL-10 inhibition. Overexpression of wild-type and constitutively active CREB in THP-1 cells revealed attenuation of the inhibitory effect of CRP on LPS-induced IL-10 levels. CRP also inhibited hemoglobin:haptoglobin-induced IL-10 and heme oxygenase-1. Furthermore, administration of human CRP to rats significantly decreased IL-10 levels. CONCLUSIONS - This study provides novel evidence that CRP, by decreasing IL-10 alters the antiinflammatory/ proinflammatory balance, accentuating inflammation, which is pivotal in atherothrombosis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2469-2475 |
| Number of pages | 7 |
| Journal | Arteriosclerosis, Thrombosis, and Vascular Biology |
| Volume | 26 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 2006 |
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Keywords
- Antiinflammatory cytokine
- Atherosclerosis
- CRP
- HMDM
- IL-10
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
C-Reactive protein decreases interleukin-10 secretion in activated human monocyte-derived macrophages via inhibition of cyclic AMP production. / Singh, Uma; Devaraj, Sridevi; Dasu, Mohan R.; Ciobanu, Dana; Reusch, Jane; Jialal, Ishwarlal.
In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 26, No. 11, 11.2006, p. 2469-2475.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - C-Reactive protein decreases interleukin-10 secretion in activated human monocyte-derived macrophages via inhibition of cyclic AMP production
AU - Singh, Uma
AU - Devaraj, Sridevi
AU - Dasu, Mohan R.
AU - Ciobanu, Dana
AU - Reusch, Jane
AU - Jialal, Ishwarlal
PY - 2006/11
Y1 - 2006/11
N2 - OBJECTIVE - C-Reactive protein (CRP), a cardiovascular risk marker, could also participate in atherosclerosis. Atherosclerotic plaques express CRP and interleukin (IL)-10, a major antiinflammatory cytokine. IL-10 deficiency results in increased lesion formation, whereas IL-10 delivery attenuates lesions. We tested the effect of CRP on lipopolysaccharide (LPS)-induced IL-10 secretion in human monocyte-derived macrophages (HMDMs). METHODS AND RESULTS - Incubation of HMDMs with CRP significantly decreased LPS-induced IL-10 mRNA and intracellular and secreted IL-10 protein and destabilized IL-10 mRNA. Also, CRP alone increased secretion of IL-8, IL-6, and tumor necrosis factor from HMDMs and did not inhibit LPS-induced secretion of these cytokines. Fc γ receptor I antibodies significantly reversed CRP-mediated IL-10 inhibition. CRP significantly decreased intracellular cAMP, phospho-cAMP response element binding protein (pCREB), and adenyl cyclase activity. cAMP agonists reversed CRP-mediated IL-10 inhibition. Overexpression of wild-type and constitutively active CREB in THP-1 cells revealed attenuation of the inhibitory effect of CRP on LPS-induced IL-10 levels. CRP also inhibited hemoglobin:haptoglobin-induced IL-10 and heme oxygenase-1. Furthermore, administration of human CRP to rats significantly decreased IL-10 levels. CONCLUSIONS - This study provides novel evidence that CRP, by decreasing IL-10 alters the antiinflammatory/ proinflammatory balance, accentuating inflammation, which is pivotal in atherothrombosis.
AB - OBJECTIVE - C-Reactive protein (CRP), a cardiovascular risk marker, could also participate in atherosclerosis. Atherosclerotic plaques express CRP and interleukin (IL)-10, a major antiinflammatory cytokine. IL-10 deficiency results in increased lesion formation, whereas IL-10 delivery attenuates lesions. We tested the effect of CRP on lipopolysaccharide (LPS)-induced IL-10 secretion in human monocyte-derived macrophages (HMDMs). METHODS AND RESULTS - Incubation of HMDMs with CRP significantly decreased LPS-induced IL-10 mRNA and intracellular and secreted IL-10 protein and destabilized IL-10 mRNA. Also, CRP alone increased secretion of IL-8, IL-6, and tumor necrosis factor from HMDMs and did not inhibit LPS-induced secretion of these cytokines. Fc γ receptor I antibodies significantly reversed CRP-mediated IL-10 inhibition. CRP significantly decreased intracellular cAMP, phospho-cAMP response element binding protein (pCREB), and adenyl cyclase activity. cAMP agonists reversed CRP-mediated IL-10 inhibition. Overexpression of wild-type and constitutively active CREB in THP-1 cells revealed attenuation of the inhibitory effect of CRP on LPS-induced IL-10 levels. CRP also inhibited hemoglobin:haptoglobin-induced IL-10 and heme oxygenase-1. Furthermore, administration of human CRP to rats significantly decreased IL-10 levels. CONCLUSIONS - This study provides novel evidence that CRP, by decreasing IL-10 alters the antiinflammatory/ proinflammatory balance, accentuating inflammation, which is pivotal in atherothrombosis.
KW - Antiinflammatory cytokine
KW - Atherosclerosis
KW - CRP
KW - HMDM
KW - IL-10
UR - http://www.scopus.com/inward/record.url?scp=33750201265&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33750201265&partnerID=8YFLogxK
U2 - 10.1161/01.ATV.0000241572.05292.fb
DO - 10.1161/01.ATV.0000241572.05292.fb
M3 - Article
VL - 26
SP - 2469
EP - 2475
JO - Arteriosclerosis, Thrombosis, and Vascular Biology
JF - Arteriosclerosis, Thrombosis, and Vascular Biology
SN - 1079-5642
IS - 11
ER -