C-O bond formation by polyketide synthases

Hyung Jin Kwon, Wyatt C. Smith, A. Janelle Scharon, Hee Hwang Sung, Mark J. Kurth, Ben Shen

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

Polyketide synthases (PKSs) assemble the polyketide carbon backbone by sequential decarboxylative condensation of acyl coenzyme A (CoA) precursors, and the C-C bond-forming step in this process is catalyzed by the β-ketoacyl synthase (KS) domain or subunit. Genetic and biochemical characterization of the nonactin biosynthesis gene cluster from Streptomyces griseus revealed two KSs, NonJ and NonK, that are highly homologous to known KSs but catalyze sequential condensation of the acyl CoA substrates by forming C-O rather than C-C bonds. This chemistry can be used in PKS engineering to increase the scope and diversity of polyketide biosynthesis.

Original languageEnglish (US)
Pages (from-to)1327-1330
Number of pages4
JournalScience
Volume297
Issue number5585
DOIs
StatePublished - Aug 23 2002
Externally publishedYes

ASJC Scopus subject areas

  • General

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    Kwon, H. J., Smith, W. C., Scharon, A. J., Sung, H. H., Kurth, M. J., & Shen, B. (2002). C-O bond formation by polyketide synthases. Science, 297(5585), 1327-1330. https://doi.org/10.1126/science.1073175