C-O bond formation by polyketide synthases

Hyung Jin Kwon, Wyatt C. Smith, A. Janelle Scharon, Hee Hwang Sung, Mark J. Kurth, Ben Shen

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Polyketide synthases (PKSs) assemble the polyketide carbon backbone by sequential decarboxylative condensation of acyl coenzyme A (CoA) precursors, and the C-C bond-forming step in this process is catalyzed by the β-ketoacyl synthase (KS) domain or subunit. Genetic and biochemical characterization of the nonactin biosynthesis gene cluster from Streptomyces griseus revealed two KSs, NonJ and NonK, that are highly homologous to known KSs but catalyze sequential condensation of the acyl CoA substrates by forming C-O rather than C-C bonds. This chemistry can be used in PKS engineering to increase the scope and diversity of polyketide biosynthesis.

Original languageEnglish (US)
Pages (from-to)1327-1330
Number of pages4
JournalScience
Volume297
Issue number5585
DOIs
StatePublished - Aug 23 2002
Externally publishedYes

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Polyketide Synthases
Polyketides
Acyl Coenzyme A
Streptomyces griseus
Multigene Family
Molecular Biology
Carbon
nonactin

ASJC Scopus subject areas

  • General

Cite this

Kwon, H. J., Smith, W. C., Scharon, A. J., Sung, H. H., Kurth, M. J., & Shen, B. (2002). C-O bond formation by polyketide synthases. Science, 297(5585), 1327-1330. https://doi.org/10.1126/science.1073175

C-O bond formation by polyketide synthases. / Kwon, Hyung Jin; Smith, Wyatt C.; Scharon, A. Janelle; Sung, Hee Hwang; Kurth, Mark J.; Shen, Ben.

In: Science, Vol. 297, No. 5585, 23.08.2002, p. 1327-1330.

Research output: Contribution to journalArticle

Kwon, HJ, Smith, WC, Scharon, AJ, Sung, HH, Kurth, MJ & Shen, B 2002, 'C-O bond formation by polyketide synthases', Science, vol. 297, no. 5585, pp. 1327-1330. https://doi.org/10.1126/science.1073175
Kwon HJ, Smith WC, Scharon AJ, Sung HH, Kurth MJ, Shen B. C-O bond formation by polyketide synthases. Science. 2002 Aug 23;297(5585):1327-1330. https://doi.org/10.1126/science.1073175
Kwon, Hyung Jin ; Smith, Wyatt C. ; Scharon, A. Janelle ; Sung, Hee Hwang ; Kurth, Mark J. ; Shen, Ben. / C-O bond formation by polyketide synthases. In: Science. 2002 ; Vol. 297, No. 5585. pp. 1327-1330.
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