c-H-ras-1 expression in 7,12-dimethyl benzanthracene-induced Balb/c mouse mammary hyperplasias and their tumors

Robert Cardiff, P. H. Gumerlock, M. M. Soong, Satya Dandekar, Peter A Barry, L. J T Young, Frederick J Meyers

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25 Scopus citations


Dimethylbenzanthracene (DMBA)-induced mouse mammary tumors characteristically contain an activated c-H-ras-1 gene with an A→T transversion in the 61st codon which is expressed as a rapidly migrating p21 c-H-ras. The role of the activated c-H-ras-1 locus in the initiation of mammary neoplasia was analysed by studying seven transplantable, DMBA-induced Balb/c premalignant mammary hyperplasias. The DNAs from these hyperplasias had no evidence of activation of the c-H-ras-1 gene. One of the fifteen tumors arising from untreated hyperplasia outgrowths had a 61st codon mutation. In contrast,67% of the tumors developing in DMBA-treated hyperplasias had the characteristic 61 codon mutation. All of the tumors with the characteristic mutation expressed the corresponding rapid p21 c-H-ras. These data suggest that although c-H-ras-1 activation may play an important role in neoplastic progression, it had no definable role in the initiation or the maintenance of the mammary hyperplasias studied here.

Original languageEnglish (US)
Pages (from-to)205-213
Number of pages9
Issue number2
StatePublished - 1988


ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

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