c-fos proto-oncogene expression in astrocytes associated with differentiation or proliferation but not depolarization

Kinya Hisanaga, Stephen M. Sagar, Katy J. Hicks, Raymond A. Swanson, Frank R Sharp

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

Expression of the c-fos proto-oncogene in rat neocortical astrocytes in culture was examined using Northern blotting and immunocytochemistry. Marked induction of c-fos mRNA in astrocytes was observed after treatment with epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), dibutyryl cyclic AMP (db-cAMP), and phorbol diester (TPA; 12-O-tetra-decanoylphorbol 13-acetate), which are known to induce the proliferation or differentiation of astrocytes. Increase of c-fos protein immunoreactivity (IR) was obtained after treatment with fetal calf serum, EGF, bFGF, db-cAMP and TPA. High concentrations of calcium ionophore A23187, which were lethal to cultured astrocytes, also increased c-fos protein-IR. Treatment with lower concentrations of calcium ionophore (which slightly increase Ca2+ uptake), higk K+ and nerve growth factor had no detectable effect on c-fos expression. These results show that depolarization does not induce c-fos in astrocytes and suggest that c-fos may play a role in differentiation and proliferation of astrocytes.

Original languageEnglish (US)
Pages (from-to)69-75
Number of pages7
JournalMolecular Brain Research
Volume8
Issue number1
DOIs
StatePublished - 1990
Externally publishedYes

Keywords

  • Astrocyte culture
  • c-fos
  • Cyclic AMP
  • Epidermal growth factor
  • Phorbol ester
  • Protein kinase
  • Proto-oncogene

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'c-fos proto-oncogene expression in astrocytes associated with differentiation or proliferation but not depolarization'. Together they form a unique fingerprint.

  • Cite this