Abstract
Expression of the c-fos proto-oncogene in rat neocortical astrocytes in culture was examined using Northern blotting and immunocytochemistry. Marked induction of c-fos mRNA in astrocytes was observed after treatment with epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), dibutyryl cyclic AMP (db-cAMP), and phorbol diester (TPA; 12-O-tetra-decanoylphorbol 13-acetate), which are known to induce the proliferation or differentiation of astrocytes. Increase of c-fos protein immunoreactivity (IR) was obtained after treatment with fetal calf serum, EGF, bFGF, db-cAMP and TPA. High concentrations of calcium ionophore A23187, which were lethal to cultured astrocytes, also increased c-fos protein-IR. Treatment with lower concentrations of calcium ionophore (which slightly increase Ca2+ uptake), higk K+ and nerve growth factor had no detectable effect on c-fos expression. These results show that depolarization does not induce c-fos in astrocytes and suggest that c-fos may play a role in differentiation and proliferation of astrocytes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 69-75 |
| Number of pages | 7 |
| Journal | Molecular Brain Research |
| Volume | 8 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1990 |
| Externally published | Yes |
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Keywords
- Astrocyte culture
- c-fos
- Cyclic AMP
- Epidermal growth factor
- Phorbol ester
- Protein kinase
- Proto-oncogene
ASJC Scopus subject areas
- Molecular Biology
- Cellular and Molecular Neuroscience
Cite this
c-fos proto-oncogene expression in astrocytes associated with differentiation or proliferation but not depolarization. / Hisanaga, Kinya; Sagar, Stephen M.; Hicks, Katy J.; Swanson, Raymond A.; Sharp, Frank R.
In: Molecular Brain Research, Vol. 8, No. 1, 1990, p. 69-75.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - c-fos proto-oncogene expression in astrocytes associated with differentiation or proliferation but not depolarization
AU - Hisanaga, Kinya
AU - Sagar, Stephen M.
AU - Hicks, Katy J.
AU - Swanson, Raymond A.
AU - Sharp, Frank R
PY - 1990
Y1 - 1990
N2 - Expression of the c-fos proto-oncogene in rat neocortical astrocytes in culture was examined using Northern blotting and immunocytochemistry. Marked induction of c-fos mRNA in astrocytes was observed after treatment with epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), dibutyryl cyclic AMP (db-cAMP), and phorbol diester (TPA; 12-O-tetra-decanoylphorbol 13-acetate), which are known to induce the proliferation or differentiation of astrocytes. Increase of c-fos protein immunoreactivity (IR) was obtained after treatment with fetal calf serum, EGF, bFGF, db-cAMP and TPA. High concentrations of calcium ionophore A23187, which were lethal to cultured astrocytes, also increased c-fos protein-IR. Treatment with lower concentrations of calcium ionophore (which slightly increase Ca2+ uptake), higk K+ and nerve growth factor had no detectable effect on c-fos expression. These results show that depolarization does not induce c-fos in astrocytes and suggest that c-fos may play a role in differentiation and proliferation of astrocytes.
AB - Expression of the c-fos proto-oncogene in rat neocortical astrocytes in culture was examined using Northern blotting and immunocytochemistry. Marked induction of c-fos mRNA in astrocytes was observed after treatment with epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), dibutyryl cyclic AMP (db-cAMP), and phorbol diester (TPA; 12-O-tetra-decanoylphorbol 13-acetate), which are known to induce the proliferation or differentiation of astrocytes. Increase of c-fos protein immunoreactivity (IR) was obtained after treatment with fetal calf serum, EGF, bFGF, db-cAMP and TPA. High concentrations of calcium ionophore A23187, which were lethal to cultured astrocytes, also increased c-fos protein-IR. Treatment with lower concentrations of calcium ionophore (which slightly increase Ca2+ uptake), higk K+ and nerve growth factor had no detectable effect on c-fos expression. These results show that depolarization does not induce c-fos in astrocytes and suggest that c-fos may play a role in differentiation and proliferation of astrocytes.
KW - Astrocyte culture
KW - c-fos
KW - Cyclic AMP
KW - Epidermal growth factor
KW - Phorbol ester
KW - Protein kinase
KW - Proto-oncogene
UR - http://www.scopus.com/inward/record.url?scp=0025346182&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025346182&partnerID=8YFLogxK
U2 - 10.1016/0169-328X(90)90011-2
DO - 10.1016/0169-328X(90)90011-2
M3 - Article
C2 - 2166202
AN - SCOPUS:0025346182
VL - 8
SP - 69
EP - 75
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1
ER -