c-fos proto-oncogene expression in astrocytes associated with differentiation or proliferation but not depolarization

Kinya Hisanaga; Stephen M. Sagar; Katy J. Hicks; Raymond A. Swanson; Frank R Sharp

doi:10.1016/0169-328X(90)90011-2

c-fos proto-oncogene expression in astrocytes associated with differentiation or proliferation but not depolarization

Kinya Hisanaga, Stephen M. Sagar, Katy J. Hicks, Raymond A. Swanson, Frank R Sharp

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

Expression of the c-fos proto-oncogene in rat neocortical astrocytes in culture was examined using Northern blotting and immunocytochemistry. Marked induction of c-fos mRNA in astrocytes was observed after treatment with epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), dibutyryl cyclic AMP (db-cAMP), and phorbol diester (TPA; 12-O-tetra-decanoylphorbol 13-acetate), which are known to induce the proliferation or differentiation of astrocytes. Increase of c-fos protein immunoreactivity (IR) was obtained after treatment with fetal calf serum, EGF, bFGF, db-cAMP and TPA. High concentrations of calcium ionophore A23187, which were lethal to cultured astrocytes, also increased c-fos protein-IR. Treatment with lower concentrations of calcium ionophore (which slightly increase Ca²⁺ uptake), higk K⁺ and nerve growth factor had no detectable effect on c-fos expression. These results show that depolarization does not induce c-fos in astrocytes and suggest that c-fos may play a role in differentiation and proliferation of astrocytes.

Original language	English (US)
Pages (from-to)	69-75
Number of pages	7
Journal	Molecular Brain Research
Volume	8
Issue number	1
DOIs	https://doi.org/10.1016/0169-328X(90)90011-2
State	Published - 1990
Externally published	Yes

Keywords

Astrocyte culture
c-fos
Cyclic AMP
Epidermal growth factor
Phorbol ester
Protein kinase
Proto-oncogene

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience

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title = "c-fos proto-oncogene expression in astrocytes associated with differentiation or proliferation but not depolarization",

abstract = "Expression of the c-fos proto-oncogene in rat neocortical astrocytes in culture was examined using Northern blotting and immunocytochemistry. Marked induction of c-fos mRNA in astrocytes was observed after treatment with epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), dibutyryl cyclic AMP (db-cAMP), and phorbol diester (TPA; 12-O-tetra-decanoylphorbol 13-acetate), which are known to induce the proliferation or differentiation of astrocytes. Increase of c-fos protein immunoreactivity (IR) was obtained after treatment with fetal calf serum, EGF, bFGF, db-cAMP and TPA. High concentrations of calcium ionophore A23187, which were lethal to cultured astrocytes, also increased c-fos protein-IR. Treatment with lower concentrations of calcium ionophore (which slightly increase Ca2+ uptake), higk K+ and nerve growth factor had no detectable effect on c-fos expression. These results show that depolarization does not induce c-fos in astrocytes and suggest that c-fos may play a role in differentiation and proliferation of astrocytes.",

keywords = "Astrocyte culture, c-fos, Cyclic AMP, Epidermal growth factor, Phorbol ester, Protein kinase, Proto-oncogene",

author = "Kinya Hisanaga and Sagar, {Stephen M.} and Hicks, {Katy J.} and Swanson, {Raymond A.} and Sharp, {Frank R}",

year = "1990",

doi = "10.1016/0169-328X(90)90011-2",

language = "English (US)",

volume = "8",

pages = "69--75",

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T1 - c-fos proto-oncogene expression in astrocytes associated with differentiation or proliferation but not depolarization

AU - Hisanaga, Kinya

AU - Sagar, Stephen M.

AU - Hicks, Katy J.

AU - Swanson, Raymond A.

AU - Sharp, Frank R

PY - 1990

Y1 - 1990

N2 - Expression of the c-fos proto-oncogene in rat neocortical astrocytes in culture was examined using Northern blotting and immunocytochemistry. Marked induction of c-fos mRNA in astrocytes was observed after treatment with epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), dibutyryl cyclic AMP (db-cAMP), and phorbol diester (TPA; 12-O-tetra-decanoylphorbol 13-acetate), which are known to induce the proliferation or differentiation of astrocytes. Increase of c-fos protein immunoreactivity (IR) was obtained after treatment with fetal calf serum, EGF, bFGF, db-cAMP and TPA. High concentrations of calcium ionophore A23187, which were lethal to cultured astrocytes, also increased c-fos protein-IR. Treatment with lower concentrations of calcium ionophore (which slightly increase Ca2+ uptake), higk K+ and nerve growth factor had no detectable effect on c-fos expression. These results show that depolarization does not induce c-fos in astrocytes and suggest that c-fos may play a role in differentiation and proliferation of astrocytes.

AB - Expression of the c-fos proto-oncogene in rat neocortical astrocytes in culture was examined using Northern blotting and immunocytochemistry. Marked induction of c-fos mRNA in astrocytes was observed after treatment with epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), dibutyryl cyclic AMP (db-cAMP), and phorbol diester (TPA; 12-O-tetra-decanoylphorbol 13-acetate), which are known to induce the proliferation or differentiation of astrocytes. Increase of c-fos protein immunoreactivity (IR) was obtained after treatment with fetal calf serum, EGF, bFGF, db-cAMP and TPA. High concentrations of calcium ionophore A23187, which were lethal to cultured astrocytes, also increased c-fos protein-IR. Treatment with lower concentrations of calcium ionophore (which slightly increase Ca2+ uptake), higk K+ and nerve growth factor had no detectable effect on c-fos expression. These results show that depolarization does not induce c-fos in astrocytes and suggest that c-fos may play a role in differentiation and proliferation of astrocytes.

KW - Astrocyte culture

KW - c-fos

KW - Cyclic AMP

KW - Epidermal growth factor

KW - Phorbol ester

KW - Protein kinase

KW - Proto-oncogene

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c-fos proto-oncogene expression in astrocytes associated with differentiation or proliferation but not depolarization

Abstract

Keywords

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