c-erbB activation in avian leukosis virus-induced erythroblastosis: multiple epidermal growth factor receptor mRNAs are generated by alternative RNA processing.

R. G. Goodwin, F. M. Rottman, T. Callaghan, Hsing-Jien Kung, P. A. Maroney, T. W. Nilsen

Research output: Contribution to journalArticle

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Abstract

Avian leukosis virus-induced erythroblastosis results from the specific interruption of the host oncogene, c-erbB, by the insertion of an intact provirus. This insertion results in the expression of two size classes (3.6 and 7.0 kilobases [kb]) of truncated c-erbB transcripts which are initiated in the 5' long terminal repeat of the integrated provirus. Through sequence analysis of erbB cDNA clones we have previously shown that the 3.6-kb activated erbB mRNA contains portions of viral gag and env genes fused to c-erbB sequences (T.W. Nilsen, P.A. Maroney, R.G. Goodwin, F.M. Rottman, L.B. Crittenden, M.A. Raines, and H.-J. Kung, Cell 41:719-726, 1985). In this report we show that the 7-kb mRNA differs from the shorter activated c-erbB mRNA in the length of its 3' untranslated sequence such that the longer mRNA has an extremely long (4.3 kb) 3' untranslated sequence. Additionally, we demonstrate that activated c-erbB mRNA precursors can be processed by alternative splicing to yield mRNAs with viral gag sequences fused directly to c-erbB sequences. Finally, blot hybridization evidence suggests that the two size classes of activated c-erbB mRNA in erythroblastic tissue represent truncated versions of the two c-erbB mRNAs present in normal tissue.

Original languageEnglish (US)
Pages (from-to)3128-3133
Number of pages6
JournalMolecular and Cellular Biology
Volume6
Issue number9
StatePublished - Sep 1986
Externally publishedYes

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Avian Leukosis Virus
Epidermal Growth Factor Receptor
RNA
Messenger RNA
Proviruses
gag Genes
env Genes
Viral Genes
Terminal Repeat Sequences
RNA Precursors
Alternative Splicing
Oncogenes
Sequence Analysis
Complementary DNA
Clone Cells

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

c-erbB activation in avian leukosis virus-induced erythroblastosis : multiple epidermal growth factor receptor mRNAs are generated by alternative RNA processing. / Goodwin, R. G.; Rottman, F. M.; Callaghan, T.; Kung, Hsing-Jien; Maroney, P. A.; Nilsen, T. W.

In: Molecular and Cellular Biology, Vol. 6, No. 9, 09.1986, p. 3128-3133.

Research output: Contribution to journalArticle

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