Butyric acid enhances in vivo expression of hTNF-α in transduced melanoma cell line

Adam Giermasz, Tomasz Grzela, Dominika Nowis, Marcin Makowski, Anna Czajka, Tomasz Stoklosa, Witold Lasek, Anna Dabrowska, Maciej Wiznerowicz, Andrzej Mackiewicz, Marek Jakóbisiak

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Butyric acid (NaBut) and its derivatives are well-known agents eliciting tumor cell differentiation and apoptosis. In experimental models, NaBut is also used to enhance the efficacy of viral vectors. With the use of B78 murine melanoma cells transduced with the retroviral vector containing human tumor necrosis factor a (hTNF-α) gene, we investigated the ability of NaBut to increase the cytokine expression. We observed an increase in hTNF-α expression in vitro after incubation with NaBut. We also describe that the NaBut pro-drug tributyrin is able to increase hTNF-α expression in transduced B78 cells in a tumor vaccination model in mice. This observation strongly suggests a novel potential role for NaBut and its derivatives in tumor therapy. It could be used not only as a therapeutic directly acting on tumor cells but, in parallel, as a genetic vaccine "enhancer".

Original languageEnglish (US)
Pages (from-to)4001-4004
Number of pages4
JournalAnticancer Research
Volume21
Issue number6 A
StatePublished - 2001
Externally publishedYes

Fingerprint

Butyric Acid
Melanoma
Cell Line
Neoplasms
Prodrugs
Cell Differentiation
Vaccination
Theoretical Models
Vaccines
Apoptosis
Cytokines
human TNF protein
Therapeutics
Genes

Keywords

  • Cancer
  • Retroviral vector
  • Sodium butyrate
  • TNF-α
  • Tributyrin
  • Vaccine

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Giermasz, A., Grzela, T., Nowis, D., Makowski, M., Czajka, A., Stoklosa, T., ... Jakóbisiak, M. (2001). Butyric acid enhances in vivo expression of hTNF-α in transduced melanoma cell line. Anticancer Research, 21(6 A), 4001-4004.

Butyric acid enhances in vivo expression of hTNF-α in transduced melanoma cell line. / Giermasz, Adam; Grzela, Tomasz; Nowis, Dominika; Makowski, Marcin; Czajka, Anna; Stoklosa, Tomasz; Lasek, Witold; Dabrowska, Anna; Wiznerowicz, Maciej; Mackiewicz, Andrzej; Jakóbisiak, Marek.

In: Anticancer Research, Vol. 21, No. 6 A, 2001, p. 4001-4004.

Research output: Contribution to journalArticle

Giermasz, A, Grzela, T, Nowis, D, Makowski, M, Czajka, A, Stoklosa, T, Lasek, W, Dabrowska, A, Wiznerowicz, M, Mackiewicz, A & Jakóbisiak, M 2001, 'Butyric acid enhances in vivo expression of hTNF-α in transduced melanoma cell line', Anticancer Research, vol. 21, no. 6 A, pp. 4001-4004.
Giermasz A, Grzela T, Nowis D, Makowski M, Czajka A, Stoklosa T et al. Butyric acid enhances in vivo expression of hTNF-α in transduced melanoma cell line. Anticancer Research. 2001;21(6 A):4001-4004.
Giermasz, Adam ; Grzela, Tomasz ; Nowis, Dominika ; Makowski, Marcin ; Czajka, Anna ; Stoklosa, Tomasz ; Lasek, Witold ; Dabrowska, Anna ; Wiznerowicz, Maciej ; Mackiewicz, Andrzej ; Jakóbisiak, Marek. / Butyric acid enhances in vivo expression of hTNF-α in transduced melanoma cell line. In: Anticancer Research. 2001 ; Vol. 21, No. 6 A. pp. 4001-4004.
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