Burn injury-mediated alterations in cell cycle progression in lymphoid organs of mice.

Kiho Cho, Lee K. Adamson, Jaehak Park, David G Greenhalgh

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A key event in the cellular and molecular pathogenesis of multiple organ failure (MOF) after burn injury may be the change in profiles of the cell cycle progression in affected organs. We investigated the effects of burn injury on cell cycle progression in immune organs. Cell cycle analysis in the lymphoid tissues of mice after 18% burn injury revealed that S phase entry was temporarily arrested in the thymus 1 day after injury, whereas the spleen had substantially increased S phase entry at day 8. This mode of cell cycle regulation was reproduced in different age groups and strains of mice. Furthermore, the reactivity to the Ki-67 antibody (indicative of proliferation) was markedly reduced in the thymic cortex at day 1. There was a distinct pattern of hematopoietic foci formation and increased reactivities to the Ki-67 antibody in myelogenous cells in the red pulp of spleen at day 7, consistent with the elevated S phase entry. These data suggest that differential regulation of cell cycle progression may play a crucial role in the phenotypic changes in immune organs after burn injury.

Original languageEnglish (US)
Pages (from-to)138-143
Number of pages6
JournalShock (Augusta, Ga.)
Volume19
Issue number2
StatePublished - Feb 2003
Externally publishedYes

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Cell Cycle
S Phase
Wounds and Injuries
Spleen
Multiple Organ Failure
Antibodies
Lymphoid Tissue
Thymus Gland
Age Groups

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Physiology

Cite this

Burn injury-mediated alterations in cell cycle progression in lymphoid organs of mice. / Cho, Kiho; Adamson, Lee K.; Park, Jaehak; Greenhalgh, David G.

In: Shock (Augusta, Ga.), Vol. 19, No. 2, 02.2003, p. 138-143.

Research output: Contribution to journalArticle

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