Abstract
Saccharomyces cerevisiae has been an important model system for numerous cellular, genetic, and molecular studies. However, this small eukaryote presents a challenge for imaging at the electron microscope level. Preparation of yeast using high-pressure freezing followed by freeze-substitution (HPF/FS) results in excellent preservation of cell structure in these difficult-to-fix samples. In particular, cells prepared by HPF/FS can be used for 3D electron tomography (ET) studies where optimum cell preservation is critical. Here, we discuss the advantages of using HPF/FS for ET and show examples of the utility of this method for building yeast cell structures in three dimensions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 183-187 |
| Number of pages | 5 |
| Journal | Cold Spring Harbor Protocols |
| Volume | 2017 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 1 2017 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Building cell structures in three dimensions : Electron tomography methods for budding yeast. / O’Toole, Eileen T.; Giddings, Thomas H.; Winey, Mark.
In: Cold Spring Harbor Protocols, Vol. 2017, No. 3, 01.03.2017, p. 183-187.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Building cell structures in three dimensions
T2 - Electron tomography methods for budding yeast
AU - O’Toole, Eileen T.
AU - Giddings, Thomas H.
AU - Winey, Mark
PY - 2017/3/1
Y1 - 2017/3/1
N2 - Saccharomyces cerevisiae has been an important model system for numerous cellular, genetic, and molecular studies. However, this small eukaryote presents a challenge for imaging at the electron microscope level. Preparation of yeast using high-pressure freezing followed by freeze-substitution (HPF/FS) results in excellent preservation of cell structure in these difficult-to-fix samples. In particular, cells prepared by HPF/FS can be used for 3D electron tomography (ET) studies where optimum cell preservation is critical. Here, we discuss the advantages of using HPF/FS for ET and show examples of the utility of this method for building yeast cell structures in three dimensions.
AB - Saccharomyces cerevisiae has been an important model system for numerous cellular, genetic, and molecular studies. However, this small eukaryote presents a challenge for imaging at the electron microscope level. Preparation of yeast using high-pressure freezing followed by freeze-substitution (HPF/FS) results in excellent preservation of cell structure in these difficult-to-fix samples. In particular, cells prepared by HPF/FS can be used for 3D electron tomography (ET) studies where optimum cell preservation is critical. Here, we discuss the advantages of using HPF/FS for ET and show examples of the utility of this method for building yeast cell structures in three dimensions.
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UR - http://www.scopus.com/inward/citedby.url?scp=85014721362&partnerID=8YFLogxK
U2 - 10.1101/pdb.top077685
DO - 10.1101/pdb.top077685
M3 - Article
VL - 2017
SP - 183
EP - 187
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
SN - 1559-6095
IS - 3
ER -