Building cell structures in three dimensions

Electron tomography methods for budding yeast

Eileen T. O’Toole, Thomas H. Giddings, Mark Winey

Research output: Contribution to journalArticle

Abstract

Saccharomyces cerevisiae has been an important model system for numerous cellular, genetic, and molecular studies. However, this small eukaryote presents a challenge for imaging at the electron microscope level. Preparation of yeast using high-pressure freezing followed by freeze-substitution (HPF/FS) results in excellent preservation of cell structure in these difficult-to-fix samples. In particular, cells prepared by HPF/FS can be used for 3D electron tomography (ET) studies where optimum cell preservation is critical. Here, we discuss the advantages of using HPF/FS for ET and show examples of the utility of this method for building yeast cell structures in three dimensions.

Original languageEnglish (US)
Pages (from-to)183-187
Number of pages5
JournalCold Spring Harbor Protocols
Volume2017
Issue number3
DOIs
StatePublished - Mar 1 2017
Externally publishedYes

Fingerprint

Electron Microscope Tomography
Saccharomycetales
Freeze Substitution
Freezing
Yeast
Tomography
Substitution reactions
Electrons
Pressure
Yeasts
Electron microscopes
Eukaryota
Cells
Saccharomyces cerevisiae
Imaging techniques
Molecular Biology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Building cell structures in three dimensions : Electron tomography methods for budding yeast. / O’Toole, Eileen T.; Giddings, Thomas H.; Winey, Mark.

In: Cold Spring Harbor Protocols, Vol. 2017, No. 3, 01.03.2017, p. 183-187.

Research output: Contribution to journalArticle

O’Toole, Eileen T. ; Giddings, Thomas H. ; Winey, Mark. / Building cell structures in three dimensions : Electron tomography methods for budding yeast. In: Cold Spring Harbor Protocols. 2017 ; Vol. 2017, No. 3. pp. 183-187.
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