Brucella requires a functional Type IV secretion system to elicit innate immune responses in mice

Christelle M. Roux, Hortensia G. Rolán, Renato L. Santos, Phillip D. Beremand, Terry L. Thomas, L. Garry Adams, Renee M Tsolis

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

The virB operon, encoding a Type IV secretion system (T4SS), is essential for intracellular survival and persistent infection by Brucella spp. To better understand the role of the T4SS in evading host defence mechanisms and establishing chronic infection, we compared transcriptional profiles of the host response to infection with wild-type and virB mutant Brucella strains. Analysis of gene expression profiles in murine splenocytes 3 days after inoculation with wild-type Brucella strains revealed an inflammatory response, with a prominent upregulation of genes induced by both type I and type II interferons. Real-time RT-PCR showed that a group of genes from these pathways were induced by day 3 post infection and declined to baseline levels by day 7. In contrast, neither of the two virB mutant strains elicited a proinflammatory gene expression profile, demonstrating that the T4SS was required to trigger this response. Infection studies using type I interferon receptor knockout mice showed that a lack of type I interferon signalling did not affect Brucella replication during the first 4 weeks of infection. Thus, induction of type I interferons does not appear to be an essential mechanism by which the T4SS promotes persistent infection by Brucella.

Original languageEnglish (US)
Pages (from-to)1851-1869
Number of pages19
JournalCellular Microbiology
Volume9
Issue number7
DOIs
StatePublished - Jul 2007

Fingerprint

Interferon Type I
Brucella
Innate Immunity
Gene expression
Genes
Interferon alpha-beta Receptor
Infection
Interferon-gamma
Transcriptome
Operon
Type IV Secretion Systems
Knockout Mice
Real-Time Polymerase Chain Reaction
Up-Regulation

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Microbiology

Cite this

Brucella requires a functional Type IV secretion system to elicit innate immune responses in mice. / Roux, Christelle M.; Rolán, Hortensia G.; Santos, Renato L.; Beremand, Phillip D.; Thomas, Terry L.; Adams, L. Garry; Tsolis, Renee M.

In: Cellular Microbiology, Vol. 9, No. 7, 07.2007, p. 1851-1869.

Research output: Contribution to journalArticle

Roux, Christelle M. ; Rolán, Hortensia G. ; Santos, Renato L. ; Beremand, Phillip D. ; Thomas, Terry L. ; Adams, L. Garry ; Tsolis, Renee M. / Brucella requires a functional Type IV secretion system to elicit innate immune responses in mice. In: Cellular Microbiology. 2007 ; Vol. 9, No. 7. pp. 1851-1869.
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