Brucella abortus VirB12 is expressed during infection but is not an essential component of the type IV secretion system

Yao Hui Sun, Hortensia G. Rolán, Andreas B. Den Hartigh, David Sondervan, Renee M Tsolis

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The Brucella abortus virB operon, consisting of 11 genes, virB1 to virB11, and two putative genes, or/12 (virB12) arid orf13, encodes a type IV secretion system (T4SS) that is required for intracellular replication and persistent infection in the mouse model. This study was undertaken to determine whether or/12 (virB12) encodes an essential part of the T4SS apparatus. The virB12 gene was found to encode a 17-kDa protein, which was detected in vitro in B. abortus grown to stationary phase. Mice infected with B. abortus 1308 produced an antibody response to the protein encoded by virB12, showing that this gene is expressed during infection. Expression of virB12 was not required for survival in J774 macrophages. VirB11 was also dispensable for the persistence of B. abortus, B. melitensis, and B. suis in mice up to 4 weeks after infection, since deletion mutants lacking virB12 were recovered from splenic tissue at wild-type levels. These results show that VirB12 is not essential for the persistence of the human-pathogenic Brucella spp. in the mouse and macrophage models of infection.

Original languageEnglish (US)
Pages (from-to)6048-6054
Number of pages7
JournalInfection and Immunity
Volume73
Issue number9
DOIs
StatePublished - Sep 2005

Fingerprint

Brucella abortus
Infection
Genes
Macrophages
Brucella
Operon
Antibody Formation
Proteins
Survival
Type IV Secretion Systems

ASJC Scopus subject areas

  • Immunology

Cite this

Brucella abortus VirB12 is expressed during infection but is not an essential component of the type IV secretion system. / Sun, Yao Hui; Rolán, Hortensia G.; Den Hartigh, Andreas B.; Sondervan, David; Tsolis, Renee M.

In: Infection and Immunity, Vol. 73, No. 9, 09.2005, p. 6048-6054.

Research output: Contribution to journalArticle

Sun, Yao Hui ; Rolán, Hortensia G. ; Den Hartigh, Andreas B. ; Sondervan, David ; Tsolis, Renee M. / Brucella abortus VirB12 is expressed during infection but is not an essential component of the type IV secretion system. In: Infection and Immunity. 2005 ; Vol. 73, No. 9. pp. 6048-6054.
@article{1a1c36ac02104d6fb12d8db2dc862ec9,
title = "Brucella abortus VirB12 is expressed during infection but is not an essential component of the type IV secretion system",
abstract = "The Brucella abortus virB operon, consisting of 11 genes, virB1 to virB11, and two putative genes, or/12 (virB12) arid orf13, encodes a type IV secretion system (T4SS) that is required for intracellular replication and persistent infection in the mouse model. This study was undertaken to determine whether or/12 (virB12) encodes an essential part of the T4SS apparatus. The virB12 gene was found to encode a 17-kDa protein, which was detected in vitro in B. abortus grown to stationary phase. Mice infected with B. abortus 1308 produced an antibody response to the protein encoded by virB12, showing that this gene is expressed during infection. Expression of virB12 was not required for survival in J774 macrophages. VirB11 was also dispensable for the persistence of B. abortus, B. melitensis, and B. suis in mice up to 4 weeks after infection, since deletion mutants lacking virB12 were recovered from splenic tissue at wild-type levels. These results show that VirB12 is not essential for the persistence of the human-pathogenic Brucella spp. in the mouse and macrophage models of infection.",
author = "Sun, {Yao Hui} and Rol{\'a}n, {Hortensia G.} and {Den Hartigh}, {Andreas B.} and David Sondervan and Tsolis, {Renee M}",
year = "2005",
month = "9",
doi = "10.1128/IAI.73.9.6048-6054.2005",
language = "English (US)",
volume = "73",
pages = "6048--6054",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "9",

}

TY - JOUR

T1 - Brucella abortus VirB12 is expressed during infection but is not an essential component of the type IV secretion system

AU - Sun, Yao Hui

AU - Rolán, Hortensia G.

AU - Den Hartigh, Andreas B.

AU - Sondervan, David

AU - Tsolis, Renee M

PY - 2005/9

Y1 - 2005/9

N2 - The Brucella abortus virB operon, consisting of 11 genes, virB1 to virB11, and two putative genes, or/12 (virB12) arid orf13, encodes a type IV secretion system (T4SS) that is required for intracellular replication and persistent infection in the mouse model. This study was undertaken to determine whether or/12 (virB12) encodes an essential part of the T4SS apparatus. The virB12 gene was found to encode a 17-kDa protein, which was detected in vitro in B. abortus grown to stationary phase. Mice infected with B. abortus 1308 produced an antibody response to the protein encoded by virB12, showing that this gene is expressed during infection. Expression of virB12 was not required for survival in J774 macrophages. VirB11 was also dispensable for the persistence of B. abortus, B. melitensis, and B. suis in mice up to 4 weeks after infection, since deletion mutants lacking virB12 were recovered from splenic tissue at wild-type levels. These results show that VirB12 is not essential for the persistence of the human-pathogenic Brucella spp. in the mouse and macrophage models of infection.

AB - The Brucella abortus virB operon, consisting of 11 genes, virB1 to virB11, and two putative genes, or/12 (virB12) arid orf13, encodes a type IV secretion system (T4SS) that is required for intracellular replication and persistent infection in the mouse model. This study was undertaken to determine whether or/12 (virB12) encodes an essential part of the T4SS apparatus. The virB12 gene was found to encode a 17-kDa protein, which was detected in vitro in B. abortus grown to stationary phase. Mice infected with B. abortus 1308 produced an antibody response to the protein encoded by virB12, showing that this gene is expressed during infection. Expression of virB12 was not required for survival in J774 macrophages. VirB11 was also dispensable for the persistence of B. abortus, B. melitensis, and B. suis in mice up to 4 weeks after infection, since deletion mutants lacking virB12 were recovered from splenic tissue at wild-type levels. These results show that VirB12 is not essential for the persistence of the human-pathogenic Brucella spp. in the mouse and macrophage models of infection.

UR - http://www.scopus.com/inward/record.url?scp=23944467588&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23944467588&partnerID=8YFLogxK

U2 - 10.1128/IAI.73.9.6048-6054.2005

DO - 10.1128/IAI.73.9.6048-6054.2005

M3 - Article

C2 - 16113325

AN - SCOPUS:23944467588

VL - 73

SP - 6048

EP - 6054

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 9

ER -