BrRxLR11 – a new phylogenetic marker with high resolution in the downy mildew genus Bremia and related genera

Young Joon Choi, Joan Wong, Fabian Runge, Bagdevi Mishra, Richard W Michelmore, Marco Thines

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The genus Bremia (Peronosporaceae; Oomycete) is a widespread pathogen of Eurasian Asteraceae (Compositae). In addition to several species infecting weeds, it includes the economically important species, B. lactucae, which causes downy mildew of lettuce (Lactuca sativa). A few loci have already been successfully sequenced for this genus, but the resolution of the resultant phylogenies is insufficient to resolve the evolutionary history of Bremia. Therefore, there is a need to develop additional loci that can provide high resolution in phylogenetic inference. In this study, we report a new locus, BrRxLR11, derived from genomic data, which shows high resolving power within the genus Bremia, similar to phylogenies based on three different loci. This gene encodes a protein of 204 aa with unknown function, a below-threshold secretion signal, and an RxLR-EER motif in the c-terminal half. As only one site seems to be under positive selection, it has potential for future application in phylogenetic investigations in the economically important genus Bremia and related genera.

Original languageEnglish (US)
Pages (from-to)185-190
Number of pages6
JournalMycological Progress
Volume16
Issue number2
DOIs
StatePublished - Feb 1 2017

Fingerprint

Bremia
Lettuce
Asteraceae
downy mildew
Phylogeny
phylogeny
Oomycetes
phylogenetics
loci
secretion
weed
genomics
pathogen
History
protein
gene
Lactuca sativa
history
lettuce
Genes

Keywords

  • cox2 mtDNA
  • ITS nrLSU
  • Multi-locus phylogeny
  • Oomycetes
  • Positive selection

ASJC Scopus subject areas

  • Plant Science

Cite this

BrRxLR11 – a new phylogenetic marker with high resolution in the downy mildew genus Bremia and related genera. / Choi, Young Joon; Wong, Joan; Runge, Fabian; Mishra, Bagdevi; Michelmore, Richard W; Thines, Marco.

In: Mycological Progress, Vol. 16, No. 2, 01.02.2017, p. 185-190.

Research output: Contribution to journalArticle

Choi, Young Joon ; Wong, Joan ; Runge, Fabian ; Mishra, Bagdevi ; Michelmore, Richard W ; Thines, Marco. / BrRxLR11 – a new phylogenetic marker with high resolution in the downy mildew genus Bremia and related genera. In: Mycological Progress. 2017 ; Vol. 16, No. 2. pp. 185-190.
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