Broad conservation of milk utilization genes in Bifidobacterium longum subsp. infantis as revealed by comparative genomic hybridization

Riccardo G. LoCascio, Prerak Desai, David A. Sela, Bart C Weimer, David A. Mills

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Human milk oligosaccharides (HMOs) are the third-largest solid component of milk. Their structural complexity renders them nondigestible to the host but liable to hydrolytic enzymes of the infant colonic microbiota. Bifidobacteria and, frequently, Bifidobacterium longum strains predominate the colonic microbiota of exclusively breast-fed infants. Among the three recognized subspecies of B. longum, B. longum subsp. infantis achieves high levels of cell growth on HMOs and is associated with early colonization of the infant gut. The B. longum subsp. infantis ATCC 15697 genome features five distinct gene clusters with the predicted capacity to bind, cleave, and import milk oligosaccharides. Comparative genomic hybridizations (CGHs) were used to associate genotypic biomarkers among 15 B. longum strains exhibiting various HMO utilization phenotypes and host associations. Multilocus sequence typing provided taxonomic subspecies designations and grouped the strains between B. longum subsp. infantis and B. longum subsp. longum. CGH analysis determined that HMO utilization gene regions are exclusively conserved across all B. longum subsp. infantis strains capable of growth on HMOs and have diverged in B. longum subsp. longum strains that cannot grow on HMOs. These regions contain fucosidases, sialidases, glycosyl hydrolases, ABC transporters, and family 1 solute binding proteins and are likely needed for efficient metabolism of HMOs. Urea metabolism genes and their activity were exclusively conserved in B. longum subsp. infantis. These results imply that the B. longum has at least two distinct subspecies: B. longum subsp. infantis, specialized to utilize milk carbon, and B. longum subsp. longum, specialized for plant-derived carbon metabolism.

Original languageEnglish (US)
Pages (from-to)7373-7381
Number of pages9
JournalApplied and Environmental Microbiology
Volume76
Issue number22
DOIs
StatePublished - Nov 2010

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Bifidobacterium longum subsp. infantis
comparative genomic hybridization
Comparative Genomic Hybridization
oligosaccharides
milk
breast milk
genomics
Milk
Bifidobacterium longum subsp. longum
Bifidobacterium longum
Oligosaccharides
gene
Human Milk
Genes
genes
Sulfalene
subspecies
metabolism
Microbiota
ABC transporters

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Broad conservation of milk utilization genes in Bifidobacterium longum subsp. infantis as revealed by comparative genomic hybridization. / LoCascio, Riccardo G.; Desai, Prerak; Sela, David A.; Weimer, Bart C; Mills, David A.

In: Applied and Environmental Microbiology, Vol. 76, No. 22, 11.2010, p. 7373-7381.

Research output: Contribution to journalArticle

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