BRL 34915 (cromakalim) enhances voltage-dependent K+ current in cultured rat hippocampal neurons

Dora M T Politi, Setsuo Suzuki, Michael A Rogawski

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The whole cell voltage-clamp recording technique was used to study the effects of the benzopyran antihypertensive agent BRL 34915 on voltage-dependent outward currents in cultured rat hippocampal neurons. Extracellular perfusion with BRL 34915 (10-500 μM) produced a dose-dependent increase in the sustained (minimally inactivating) voltage-dependent outward altering the transient outward current (IA) or producing a change in the resting current at -60 mV. One-half maximal facilitation of the sustained outward current occured at 40 μM and maximal facilitation (59%) at 100 μM. The increase in outward current occurred at all potentials where hippocampal neurons exhibited outward rectification (> -30 mV). The effect of BRL 34915 was slow to develop (requiring as long as 5-15 min to achieve maximal effect) and persisted for at least 10-15 min after cessation of the drug superfusion. Like the sustained outward current recorded under control conditions, the outward current augmented by BRL 34915 wasinhibited by the K+ channel blocker tetraethylammonium (20 mM). These results indicate that BRL 34915 can enhance the activity of sustained voltage-dependent K+ channels in mammalian CNS neurons.

Original languageEnglish (US)
Pages (from-to)7-14
Number of pages8
JournalEuropean Journal of Pharmacology
Volume168
Issue number1
DOIs
StatePublished - Sep 1 1989
Externally publishedYes

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Keywords

  • (Whole cell patch clamp)
  • Cromakalim (BRL 34915)
  • Hippocampus
  • K channels
  • Tetraethylammonium

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

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