Brief focal cerebral ischemia that simulates transient ischemic attacks in humans regulates gene expression in rat peripheral blood

Xinhua Zhan, Bradley Ander, Glen Jickling, Renée Turner, Boryana Stamova, Huichun Xu, Da Liu, Ryan R. Davis, Frank R Sharp

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Blood gene expression profiles of very brief (5 and 10 mins) focal ischemia that simulates transient ischemic attacks in humans were compared with ischemic stroke (120 mins focal ischemia), sham, and nave controls. The number of significantly regulated genes after 5 and 10 mins of cerebral ischemia was 39 and 160, respectively (fold change 1.5 and P0.05). There were 103 genes common to brief focal ischemia and ischemic stroke. Ingenuity pathway analysis showed that genes regulated in the 5 mins group were mainly involved in small molecule biochemistry. Genes regulated in the 10 mins group were involved in cell death, development, growth, and proliferation. Such genes were also regulated in the ischemic stroke group. Genes common to ischemia were involved in the inflammatory response, immune response, and cell deathindicating that these pathways are a feature of focal ischemia, regardless of the duration. These results provide evidence that brief focal ischemia differentially regulates gene expression in the peripheral blood in a manner that could distinguish brief focal ischemia from ischemic stroke and controls in rats. We postulate that this will also occur in humans.

Original languageEnglish (US)
Pages (from-to)110-118
Number of pages9
JournalJournal of Cerebral Blood Flow and Metabolism
Volume30
Issue number1
DOIs
StatePublished - Jan 2010

Keywords

  • Blood
  • Focal cerebral ischemia
  • Gene expression
  • Rat
  • Stroke
  • Transient ischemic attack

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Neurology

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