Abstract
Breath hydrogen (H2) concentration, an indicator of intestinal microbial abundance, was determined in cats given purified and commercial canned and dry-type diets. Before measurements, the cats were fed diets for more than 2 wk and habituated to a daily feeding interval of 4 hr. Breath H2 concentrations were determined before a meal (∼25% daily MER) and then every 20 min for 8 hr or hourly for 10 hr. A clear rise above baseline breath H2 concentrations, 1-2 ppm, was not observed in 6 males given a casein-based purified diet. A mean (± SEM) peak breath H2 concentration of 22 ± 4 ppm was observed in 6 other males, 6.3 hr after ingestion of a canned diet with protein, fat, and carbohydrate proportions similar to those of the purified diet. Area-under-the-curve (AUC) breath H2 responses to the canned diet were substantially greater (p < 0.05) than responses observed in 5 males given a dry-type diet, but similar to responses observed in 12 males given an uncooked form of the canned diet. Gamma irradiation to inactivate microbes in the uncooked diet did not affect the breath H2 response. Breath H2 responses to 2 other canned and 2 other dry-type diets were evaluated in 8 adult females using a 4 × 4 Latin-square design. Peak and AUC responses to the canned diets were similar but approximately 2 times greater (p < 0.05) than responses to the dry diets. Relative to dry-type diets, canned diets induce a substantially greater breath H2 production, and therefore appear to support a greater intestinal microbial population.
| Original language | English (US) |
|---|---|
| Journal | Journal of Nutrition |
| Volume | 132 |
| Issue number | 6 SUPPL. 1 |
| State | Published - Jun 18 2002 |
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Keywords
- Breath hydrogen concentration
- Domestic cats
- Food bacteria
- Gastrointestinal microflora
- Oral-cecal transit time
ASJC Scopus subject areas
- Food Science
- Medicine (miscellaneous)
Cite this
Breath hydrogen concentrations of cats given commercial canned and extruded diets indicate gastrointestinal microbial activity vary with diet type. / Backus, Robert C.; Puryear, Lisa M.; Crouse, Barbara A.; Biourge, Vincent C.; Rogers, Quinton.
In: Journal of Nutrition, Vol. 132, No. 6 SUPPL. 1, 18.06.2002.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Breath hydrogen concentrations of cats given commercial canned and extruded diets indicate gastrointestinal microbial activity vary with diet type
AU - Backus, Robert C.
AU - Puryear, Lisa M.
AU - Crouse, Barbara A.
AU - Biourge, Vincent C.
AU - Rogers, Quinton
PY - 2002/6/18
Y1 - 2002/6/18
N2 - Breath hydrogen (H2) concentration, an indicator of intestinal microbial abundance, was determined in cats given purified and commercial canned and dry-type diets. Before measurements, the cats were fed diets for more than 2 wk and habituated to a daily feeding interval of 4 hr. Breath H2 concentrations were determined before a meal (∼25% daily MER) and then every 20 min for 8 hr or hourly for 10 hr. A clear rise above baseline breath H2 concentrations, 1-2 ppm, was not observed in 6 males given a casein-based purified diet. A mean (± SEM) peak breath H2 concentration of 22 ± 4 ppm was observed in 6 other males, 6.3 hr after ingestion of a canned diet with protein, fat, and carbohydrate proportions similar to those of the purified diet. Area-under-the-curve (AUC) breath H2 responses to the canned diet were substantially greater (p < 0.05) than responses observed in 5 males given a dry-type diet, but similar to responses observed in 12 males given an uncooked form of the canned diet. Gamma irradiation to inactivate microbes in the uncooked diet did not affect the breath H2 response. Breath H2 responses to 2 other canned and 2 other dry-type diets were evaluated in 8 adult females using a 4 × 4 Latin-square design. Peak and AUC responses to the canned diets were similar but approximately 2 times greater (p < 0.05) than responses to the dry diets. Relative to dry-type diets, canned diets induce a substantially greater breath H2 production, and therefore appear to support a greater intestinal microbial population.
AB - Breath hydrogen (H2) concentration, an indicator of intestinal microbial abundance, was determined in cats given purified and commercial canned and dry-type diets. Before measurements, the cats were fed diets for more than 2 wk and habituated to a daily feeding interval of 4 hr. Breath H2 concentrations were determined before a meal (∼25% daily MER) and then every 20 min for 8 hr or hourly for 10 hr. A clear rise above baseline breath H2 concentrations, 1-2 ppm, was not observed in 6 males given a casein-based purified diet. A mean (± SEM) peak breath H2 concentration of 22 ± 4 ppm was observed in 6 other males, 6.3 hr after ingestion of a canned diet with protein, fat, and carbohydrate proportions similar to those of the purified diet. Area-under-the-curve (AUC) breath H2 responses to the canned diet were substantially greater (p < 0.05) than responses observed in 5 males given a dry-type diet, but similar to responses observed in 12 males given an uncooked form of the canned diet. Gamma irradiation to inactivate microbes in the uncooked diet did not affect the breath H2 response. Breath H2 responses to 2 other canned and 2 other dry-type diets were evaluated in 8 adult females using a 4 × 4 Latin-square design. Peak and AUC responses to the canned diets were similar but approximately 2 times greater (p < 0.05) than responses to the dry diets. Relative to dry-type diets, canned diets induce a substantially greater breath H2 production, and therefore appear to support a greater intestinal microbial population.
KW - Breath hydrogen concentration
KW - Domestic cats
KW - Food bacteria
KW - Gastrointestinal microflora
KW - Oral-cecal transit time
UR - http://www.scopus.com/inward/record.url?scp=0036269122&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036269122&partnerID=8YFLogxK
M3 - Article
VL - 132
JO - Journal of Nutrition
JF - Journal of Nutrition
SN - 0022-3166
IS - 6 SUPPL. 1
ER -