Breath hydrogen concentrations of cats given commercial canned and extruded diets indicate gastrointestinal microbial activity vary with diet type

Robert C. Backus, Lisa M. Puryear, Barbara A. Crouse, Vincent C. Biourge, Quinton Rogers

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Breath hydrogen (H2) concentration, an indicator of intestinal microbial abundance, was determined in cats given purified and commercial canned and dry-type diets. Before measurements, the cats were fed diets for more than 2 wk and habituated to a daily feeding interval of 4 hr. Breath H2 concentrations were determined before a meal (∼25% daily MER) and then every 20 min for 8 hr or hourly for 10 hr. A clear rise above baseline breath H2 concentrations, 1-2 ppm, was not observed in 6 males given a casein-based purified diet. A mean (± SEM) peak breath H2 concentration of 22 ± 4 ppm was observed in 6 other males, 6.3 hr after ingestion of a canned diet with protein, fat, and carbohydrate proportions similar to those of the purified diet. Area-under-the-curve (AUC) breath H2 responses to the canned diet were substantially greater (p < 0.05) than responses observed in 5 males given a dry-type diet, but similar to responses observed in 12 males given an uncooked form of the canned diet. Gamma irradiation to inactivate microbes in the uncooked diet did not affect the breath H2 response. Breath H2 responses to 2 other canned and 2 other dry-type diets were evaluated in 8 adult females using a 4 × 4 Latin-square design. Peak and AUC responses to the canned diets were similar but approximately 2 times greater (p < 0.05) than responses to the dry diets. Relative to dry-type diets, canned diets induce a substantially greater breath H2 production, and therefore appear to support a greater intestinal microbial population.

Original languageEnglish (US)
JournalJournal of Nutrition
Volume132
Issue number6 SUPPL. 1
StatePublished - Jun 18 2002

Fingerprint

microbial activity
hydrogen
Hydrogen
Cats
cats
Diet
diet
Area Under Curve
Ethamoxytriphetol
hydrogen production
Caseins
gamma radiation
Meals
casein
Eating
Fats
Carbohydrates
ingestion
carbohydrates

Keywords

  • Breath hydrogen concentration
  • Domestic cats
  • Food bacteria
  • Gastrointestinal microflora
  • Oral-cecal transit time

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

Breath hydrogen concentrations of cats given commercial canned and extruded diets indicate gastrointestinal microbial activity vary with diet type. / Backus, Robert C.; Puryear, Lisa M.; Crouse, Barbara A.; Biourge, Vincent C.; Rogers, Quinton.

In: Journal of Nutrition, Vol. 132, No. 6 SUPPL. 1, 18.06.2002.

Research output: Contribution to journalArticle

Backus, Robert C. ; Puryear, Lisa M. ; Crouse, Barbara A. ; Biourge, Vincent C. ; Rogers, Quinton. / Breath hydrogen concentrations of cats given commercial canned and extruded diets indicate gastrointestinal microbial activity vary with diet type. In: Journal of Nutrition. 2002 ; Vol. 132, No. 6 SUPPL. 1.
@article{9a5b8db80c7b496d88e73e456013dc67,
title = "Breath hydrogen concentrations of cats given commercial canned and extruded diets indicate gastrointestinal microbial activity vary with diet type",
abstract = "Breath hydrogen (H2) concentration, an indicator of intestinal microbial abundance, was determined in cats given purified and commercial canned and dry-type diets. Before measurements, the cats were fed diets for more than 2 wk and habituated to a daily feeding interval of 4 hr. Breath H2 concentrations were determined before a meal (∼25{\%} daily MER) and then every 20 min for 8 hr or hourly for 10 hr. A clear rise above baseline breath H2 concentrations, 1-2 ppm, was not observed in 6 males given a casein-based purified diet. A mean (± SEM) peak breath H2 concentration of 22 ± 4 ppm was observed in 6 other males, 6.3 hr after ingestion of a canned diet with protein, fat, and carbohydrate proportions similar to those of the purified diet. Area-under-the-curve (AUC) breath H2 responses to the canned diet were substantially greater (p < 0.05) than responses observed in 5 males given a dry-type diet, but similar to responses observed in 12 males given an uncooked form of the canned diet. Gamma irradiation to inactivate microbes in the uncooked diet did not affect the breath H2 response. Breath H2 responses to 2 other canned and 2 other dry-type diets were evaluated in 8 adult females using a 4 × 4 Latin-square design. Peak and AUC responses to the canned diets were similar but approximately 2 times greater (p < 0.05) than responses to the dry diets. Relative to dry-type diets, canned diets induce a substantially greater breath H2 production, and therefore appear to support a greater intestinal microbial population.",
keywords = "Breath hydrogen concentration, Domestic cats, Food bacteria, Gastrointestinal microflora, Oral-cecal transit time",
author = "Backus, {Robert C.} and Puryear, {Lisa M.} and Crouse, {Barbara A.} and Biourge, {Vincent C.} and Quinton Rogers",
year = "2002",
month = "6",
day = "18",
language = "English (US)",
volume = "132",
journal = "Journal of Nutrition",
issn = "0022-3166",
publisher = "American Society for Nutrition",
number = "6 SUPPL. 1",

}

TY - JOUR

T1 - Breath hydrogen concentrations of cats given commercial canned and extruded diets indicate gastrointestinal microbial activity vary with diet type

AU - Backus, Robert C.

AU - Puryear, Lisa M.

AU - Crouse, Barbara A.

AU - Biourge, Vincent C.

AU - Rogers, Quinton

PY - 2002/6/18

Y1 - 2002/6/18

N2 - Breath hydrogen (H2) concentration, an indicator of intestinal microbial abundance, was determined in cats given purified and commercial canned and dry-type diets. Before measurements, the cats were fed diets for more than 2 wk and habituated to a daily feeding interval of 4 hr. Breath H2 concentrations were determined before a meal (∼25% daily MER) and then every 20 min for 8 hr or hourly for 10 hr. A clear rise above baseline breath H2 concentrations, 1-2 ppm, was not observed in 6 males given a casein-based purified diet. A mean (± SEM) peak breath H2 concentration of 22 ± 4 ppm was observed in 6 other males, 6.3 hr after ingestion of a canned diet with protein, fat, and carbohydrate proportions similar to those of the purified diet. Area-under-the-curve (AUC) breath H2 responses to the canned diet were substantially greater (p < 0.05) than responses observed in 5 males given a dry-type diet, but similar to responses observed in 12 males given an uncooked form of the canned diet. Gamma irradiation to inactivate microbes in the uncooked diet did not affect the breath H2 response. Breath H2 responses to 2 other canned and 2 other dry-type diets were evaluated in 8 adult females using a 4 × 4 Latin-square design. Peak and AUC responses to the canned diets were similar but approximately 2 times greater (p < 0.05) than responses to the dry diets. Relative to dry-type diets, canned diets induce a substantially greater breath H2 production, and therefore appear to support a greater intestinal microbial population.

AB - Breath hydrogen (H2) concentration, an indicator of intestinal microbial abundance, was determined in cats given purified and commercial canned and dry-type diets. Before measurements, the cats were fed diets for more than 2 wk and habituated to a daily feeding interval of 4 hr. Breath H2 concentrations were determined before a meal (∼25% daily MER) and then every 20 min for 8 hr or hourly for 10 hr. A clear rise above baseline breath H2 concentrations, 1-2 ppm, was not observed in 6 males given a casein-based purified diet. A mean (± SEM) peak breath H2 concentration of 22 ± 4 ppm was observed in 6 other males, 6.3 hr after ingestion of a canned diet with protein, fat, and carbohydrate proportions similar to those of the purified diet. Area-under-the-curve (AUC) breath H2 responses to the canned diet were substantially greater (p < 0.05) than responses observed in 5 males given a dry-type diet, but similar to responses observed in 12 males given an uncooked form of the canned diet. Gamma irradiation to inactivate microbes in the uncooked diet did not affect the breath H2 response. Breath H2 responses to 2 other canned and 2 other dry-type diets were evaluated in 8 adult females using a 4 × 4 Latin-square design. Peak and AUC responses to the canned diets were similar but approximately 2 times greater (p < 0.05) than responses to the dry diets. Relative to dry-type diets, canned diets induce a substantially greater breath H2 production, and therefore appear to support a greater intestinal microbial population.

KW - Breath hydrogen concentration

KW - Domestic cats

KW - Food bacteria

KW - Gastrointestinal microflora

KW - Oral-cecal transit time

UR - http://www.scopus.com/inward/record.url?scp=0036269122&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036269122&partnerID=8YFLogxK

M3 - Article

VL - 132

JO - Journal of Nutrition

JF - Journal of Nutrition

SN - 0022-3166

IS - 6 SUPPL. 1

ER -