Breakpoint heterogeneity in t(10;11) translocation in AML-M4/M5 resulting in fusion of AF10 and MLL is resolved by fluorescent in situ hybridization analysis

H. B. Beverloo, M. Le Coniat, J. Wijsman, D. M. Lillington, O. Bernard, A. De Klein, E. Van Wering, J. Welborn, B. D. Young, A. Hagemeijer, R. Berger

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Abstract

Ten AML-M4/M5 patients' samples containing a t(10;11) translocation, but with different cytogenetic breakpoints on chromosome 11q (11q13-23), were studied by G- and R-banding and fluorescent in situ hybridization. Southern blotting analysis, studied in five patients, revealed a rearranged MLL gene. Reverse transcription-PCR analysis carried out in six patients showed a 5' MLL-3' AF-10 fusion transcript. Fluorescent in situ hybridization studies suggested that in 8 of 10 patients, the rearrangement/fusion transcript resulted from an inversion of a part of 11q (q13q23) translocated to 10p12. In the other two patients, it is assumed that an inversion/translocation has occurred of a part of 10p to the der(11). The results suggest that the orientation of the AF-10 gene on 10p is 5' telomeric and 3' centromeric. This is the first example of opposite-oriented genes being involved in translocation to yield fusion transcripts.

Original languageEnglish (US)
Pages (from-to)4220-4224
Number of pages5
JournalCancer Research
Volume55
Issue number19
StatePublished - 1995

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Beverloo, H. B., Le Coniat, M., Wijsman, J., Lillington, D. M., Bernard, O., De Klein, A., Van Wering, E., Welborn, J., Young, B. D., Hagemeijer, A., & Berger, R. (1995). Breakpoint heterogeneity in t(10;11) translocation in AML-M4/M5 resulting in fusion of AF10 and MLL is resolved by fluorescent in situ hybridization analysis. Cancer Research, 55(19), 4220-4224.