Bradykinin stimulates Cl secretion and prostaglandin E2 release by canine tracheal epithelium

G. D. Leikauf, I. F. Ueki, J. A. Nadel, Jonathan Widdicombe

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Scopus citations

Abstract

Bradykinin increased mean short-circuit (I(sc)) when added either to the mucosal (K(D) = 1.1 nM; ΔI(sc)(max) = 29.8 ± 4.4 μA/cm2) or submucosal bath (K(D) = 108 nM; ΔI(sc)(max) = 27.1 ± 4.9 μA/cm2). Bumetanide or replacement of Cl reduced the maximal change in I(sc). In paired tissues, the increase in net 36Cl flux toward the mucosa equaled the change in I(sc). Net 22Na flux toward the submucosa was unchanged. Involvement of intramural nerves was ruled out because bradykinin-induced increases in I(sc) were not inhibited by phentolamine, propranolol, atropine, or tetrodotoxin. A direct action of bradykinin on the epithelium was also made probable by the autoradiographic demonstration of specific bradykinin binding sites. The antagonists of arachidonic acid metabolism, indomethacin and eicosa-5,8,11,14-tetraynoic acid, inhibited the increase in I(sc) induced by bradykinin. Finally, prostaglandin E2 release was significantly increased by submucosal addition of bradykinin, and this effect was abolished by pretreatment with indomethacin. We conclude that bradykinin stimulates Cl secretion in canine tracheal epithelium by increasing prostaglandin release.

Original languageEnglish (US)
Title of host publicationAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume17
Edition1
StatePublished - 1985
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Bradykinin stimulates Cl secretion and prostaglandin E<sub>2</sub> release by canine tracheal epithelium'. Together they form a unique fingerprint.

  • Cite this

    Leikauf, G. D., Ueki, I. F., Nadel, J. A., & Widdicombe, J. (1985). Bradykinin stimulates Cl secretion and prostaglandin E2 release by canine tracheal epithelium. In American Journal of Physiology - Renal Fluid and Electrolyte Physiology (1 ed., Vol. 17)