Bradykinin contributes to the exercise pressor reflex: Mechanism of action

H. L. Pan, Charles L Stebbins, J. C. Longhurst

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

This study determined the receptors responsible for mediating bradykinin's effect on skeletal muscle afferents that cause the pressor reflex in anesthetized cats. In eight cats, 1 μg of bradykinin was injected intra- arterially into the gracilis muscle before and after intravenous injection of a kinin B2-receptor antagonist (NPC 17731, 20 μg/kg). Initial injection of bradykinin reflexly increased mean arterial pressure by 23 ± 7 mmHg, maximal change in pressure over time by 439 ± 272 mmHg/s, and heart rate by 11 ± 4 beats/min. The hemodynamic response to bradykinin was abolished by kinin B2- receptor blockade. Similar injection of the kinin B1-receptor agonist des- Arg9-bradykinin caused no cardiovascular responses (n = 6). In eight different animals, mean arterial pressure, maximal change in left ventricular pressure over time, and heart rate responses to 30 s of electrically stimulated hindlimb contraction were attenuated by 50 ± 6, 55 ± 7, and 41 ± 8%, respectively, after kinin B2-receptor blockade. In eight other animals, mean arterial pressure, maximal change in left ventricular pressure over time, and heart rate responses were reduced by 58 ± 8, 66 ± 6, and 40 ± 12%, respectively, after inhibition of prostaglandin synthesis with indomethacin (2.5-3 mg/kg iv) and were then abolished by subsequent B2- receptor blockade. These data suggest that bradykinin contributes to the exercise pressor reflex through its action on kinin B2 receptors located on the nerve endings of the muscle afferents. The majority of this bradykinin effect is dependent on local concentrations of prostaglandins that can augment the stimulating action of this peptide on muscle afferent nerve endings and that may be enhanced by activation of kinin B2 receptors.

Original languageEnglish (US)
Pages (from-to)2061-2068
Number of pages8
JournalJournal of Applied Physiology
Volume75
Issue number5
StatePublished - 1993

Keywords

  • indomethacin
  • kinin B antagonists
  • kinin B receptors
  • prostaglandins
  • static muscle contraction

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

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    Pan, H. L., Stebbins, C. L., & Longhurst, J. C. (1993). Bradykinin contributes to the exercise pressor reflex: Mechanism of action. Journal of Applied Physiology, 75(5), 2061-2068.