Bovine viral diarrhoea virus infection of alpacas (Vicugna pacos) in the UK

A. P. Foster, M. G. Houlihan, J. P. Holmes, E. J. Watt, Robert Higgins, J. Errington, G. Ibata, P. R. Wakeley

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Three alpacas (Vicugna pacos) aged two to 22 months with a history of illthrift and diarrhoea were examined postmortem, and tissues were collected for histology, including immunohistochemical labelling for pestivirus antigen, virus isolation and TaqMan reverse transcriptase-PCR assay. Blood samples from two clinical cases and the remaining herd members were tested for bovine viral diarrhoea virus (BVDV) antibody by serum neutralisation, antigen detection and PCR assay. The three affected alpacas were positive for BVDV by PCR of splenic tissue and/or heparinised blood. Non-cytopathic BVDV was isolated from several tissues and plasma of two of the alpacas, DNA sequencing and phylogenetic analysis of the viral genome from the PCR product showed that the BVDV was of subgenotype 1 b. Immunohistochemical examination of brain tissue was positive in two cases, consistent with a persistent infection, BVDV antibodies were detected in 16 of 25 clinically unaffected alpacas. There was no evidence of persistent infection in the in-contact animals. The source of the infection was not determined.

Original languageEnglish (US)
Pages (from-to)94-99
Number of pages6
JournalVeterinary Record
Volume161
Issue number3
DOIs
StatePublished - Jul 21 2007
Externally publishedYes

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New World Camelids
Bovine Viral Diarrhea Viruses
alpacas
Bovine viral diarrhea virus
Virus Diseases
infection
Polymerase Chain Reaction
Infection
Pestivirus
Antigens
antigen detection
antibodies
Antibodies
Viral Genome
blood
assays
blood serum
Reverse Transcriptase Polymerase Chain Reaction
DNA Sequence Analysis
neutralization

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Foster, A. P., Houlihan, M. G., Holmes, J. P., Watt, E. J., Higgins, R., Errington, J., ... Wakeley, P. R. (2007). Bovine viral diarrhoea virus infection of alpacas (Vicugna pacos) in the UK. Veterinary Record, 161(3), 94-99. https://doi.org/10.1136/vr.161.3.94

Bovine viral diarrhoea virus infection of alpacas (Vicugna pacos) in the UK. / Foster, A. P.; Houlihan, M. G.; Holmes, J. P.; Watt, E. J.; Higgins, Robert; Errington, J.; Ibata, G.; Wakeley, P. R.

In: Veterinary Record, Vol. 161, No. 3, 21.07.2007, p. 94-99.

Research output: Contribution to journalArticle

Foster, AP, Houlihan, MG, Holmes, JP, Watt, EJ, Higgins, R, Errington, J, Ibata, G & Wakeley, PR 2007, 'Bovine viral diarrhoea virus infection of alpacas (Vicugna pacos) in the UK', Veterinary Record, vol. 161, no. 3, pp. 94-99. https://doi.org/10.1136/vr.161.3.94
Foster AP, Houlihan MG, Holmes JP, Watt EJ, Higgins R, Errington J et al. Bovine viral diarrhoea virus infection of alpacas (Vicugna pacos) in the UK. Veterinary Record. 2007 Jul 21;161(3):94-99. https://doi.org/10.1136/vr.161.3.94
Foster, A. P. ; Houlihan, M. G. ; Holmes, J. P. ; Watt, E. J. ; Higgins, Robert ; Errington, J. ; Ibata, G. ; Wakeley, P. R. / Bovine viral diarrhoea virus infection of alpacas (Vicugna pacos) in the UK. In: Veterinary Record. 2007 ; Vol. 161, No. 3. pp. 94-99.
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