Bovine T cells, B cells, and null cells are transformed by the protozoan parasite Theileria parva

C. L. Baldwin, S. J. Black, W. C. Brown, Patricia A Conrad, B. M. Goddeeris, S. W. Kinuthia, P. A. Lalor, N. D. MacHugh, W. I. Morrison, S. P. Morzaria, J. Naessens, J. Newson

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Abstract

The target cells for infection and transformation by Theileria parva were investigated. Peripheral blood manonuclear cells were reacted with monoclonal antibodies specific for bovine leukocyte differentiation antigens, sorted into subpopulations with a fluorescence-activated cell sorter, and infected in vitro with T. parva sporozoites. Infected cells were cultured at limiting dilution, and transformed clones were screened with monoclonal antibodies. The results indicated that B cells, T cells (including BoT4+ and BoT8+ cells), and null cells but not monocytes or neutrophils were transformed in vitro after infection with T. parva. After transformation, peripheral blood T cells and T-cell clones retained expression of most or all of the T-cell differentiation antigens including the mature T-cell marker recognized by monoclonal antibody IL-A27, BoT2, and BoT4 or BoT8, and some cells acquired a low level of expression of BoT4, BoT8, or the null cell marker recognized by monoclonal antibody IL-A29. T. parva-transformed null cells retained expression of the IL-A29 determinant and acquired expression of BoT2 and BoT8 but not the IL-A27 determinant or BoT4. T.parva-transformed B cells in most instances lost expression of surface immunoglobulin and never acquired expression of the IL-A27 determinant, BoT2, BoT4, or BoT8, although some cells acquired a low level of expression of the null cell marker recognized by monoclonal antibody IL-A29. Further studies on cell lines and clones grown in vitro from populations isolated from T. parva-infected cattle suggested that the majority of the in vivo T. parva-transformed cells were of T-cell origin.

Original languageEnglish (US)
Pages (from-to)462-467
Number of pages6
JournalInfection and Immunity
Volume56
Issue number2
StatePublished - 1988
Externally publishedYes

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Theileria parva
Null Lymphocytes
Parasites
B-Lymphocytes
T-Lymphocytes
N,N-dimethyl-3,3-diphenyl-1-methylallylamine
Monoclonal Antibodies
Clone Cells
Blood Cells
T Lymphocyte Differentiation Antigens
Sporozoites
B-Cell Antigen Receptors
Differentiation Antigens
HLA Antigens
Infection
Monocytes
Cultured Cells
Neutrophils
Fluorescence
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

Baldwin, C. L., Black, S. J., Brown, W. C., Conrad, P. A., Goddeeris, B. M., Kinuthia, S. W., ... Newson, J. (1988). Bovine T cells, B cells, and null cells are transformed by the protozoan parasite Theileria parva. Infection and Immunity, 56(2), 462-467.

Bovine T cells, B cells, and null cells are transformed by the protozoan parasite Theileria parva. / Baldwin, C. L.; Black, S. J.; Brown, W. C.; Conrad, Patricia A; Goddeeris, B. M.; Kinuthia, S. W.; Lalor, P. A.; MacHugh, N. D.; Morrison, W. I.; Morzaria, S. P.; Naessens, J.; Newson, J.

In: Infection and Immunity, Vol. 56, No. 2, 1988, p. 462-467.

Research output: Contribution to journalArticle

Baldwin, CL, Black, SJ, Brown, WC, Conrad, PA, Goddeeris, BM, Kinuthia, SW, Lalor, PA, MacHugh, ND, Morrison, WI, Morzaria, SP, Naessens, J & Newson, J 1988, 'Bovine T cells, B cells, and null cells are transformed by the protozoan parasite Theileria parva', Infection and Immunity, vol. 56, no. 2, pp. 462-467.
Baldwin CL, Black SJ, Brown WC, Conrad PA, Goddeeris BM, Kinuthia SW et al. Bovine T cells, B cells, and null cells are transformed by the protozoan parasite Theileria parva. Infection and Immunity. 1988;56(2):462-467.
Baldwin, C. L. ; Black, S. J. ; Brown, W. C. ; Conrad, Patricia A ; Goddeeris, B. M. ; Kinuthia, S. W. ; Lalor, P. A. ; MacHugh, N. D. ; Morrison, W. I. ; Morzaria, S. P. ; Naessens, J. ; Newson, J. / Bovine T cells, B cells, and null cells are transformed by the protozoan parasite Theileria parva. In: Infection and Immunity. 1988 ; Vol. 56, No. 2. pp. 462-467.
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abstract = "The target cells for infection and transformation by Theileria parva were investigated. Peripheral blood manonuclear cells were reacted with monoclonal antibodies specific for bovine leukocyte differentiation antigens, sorted into subpopulations with a fluorescence-activated cell sorter, and infected in vitro with T. parva sporozoites. Infected cells were cultured at limiting dilution, and transformed clones were screened with monoclonal antibodies. The results indicated that B cells, T cells (including BoT4+ and BoT8+ cells), and null cells but not monocytes or neutrophils were transformed in vitro after infection with T. parva. After transformation, peripheral blood T cells and T-cell clones retained expression of most or all of the T-cell differentiation antigens including the mature T-cell marker recognized by monoclonal antibody IL-A27, BoT2, and BoT4 or BoT8, and some cells acquired a low level of expression of BoT4, BoT8, or the null cell marker recognized by monoclonal antibody IL-A29. T. parva-transformed null cells retained expression of the IL-A29 determinant and acquired expression of BoT2 and BoT8 but not the IL-A27 determinant or BoT4. T.parva-transformed B cells in most instances lost expression of surface immunoglobulin and never acquired expression of the IL-A27 determinant, BoT2, BoT4, or BoT8, although some cells acquired a low level of expression of the null cell marker recognized by monoclonal antibody IL-A29. Further studies on cell lines and clones grown in vitro from populations isolated from T. parva-infected cattle suggested that the majority of the in vivo T. parva-transformed cells were of T-cell origin.",
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T1 - Bovine T cells, B cells, and null cells are transformed by the protozoan parasite Theileria parva

AU - Baldwin, C. L.

AU - Black, S. J.

AU - Brown, W. C.

AU - Conrad, Patricia A

AU - Goddeeris, B. M.

AU - Kinuthia, S. W.

AU - Lalor, P. A.

AU - MacHugh, N. D.

AU - Morrison, W. I.

AU - Morzaria, S. P.

AU - Naessens, J.

AU - Newson, J.

PY - 1988

Y1 - 1988

N2 - The target cells for infection and transformation by Theileria parva were investigated. Peripheral blood manonuclear cells were reacted with monoclonal antibodies specific for bovine leukocyte differentiation antigens, sorted into subpopulations with a fluorescence-activated cell sorter, and infected in vitro with T. parva sporozoites. Infected cells were cultured at limiting dilution, and transformed clones were screened with monoclonal antibodies. The results indicated that B cells, T cells (including BoT4+ and BoT8+ cells), and null cells but not monocytes or neutrophils were transformed in vitro after infection with T. parva. After transformation, peripheral blood T cells and T-cell clones retained expression of most or all of the T-cell differentiation antigens including the mature T-cell marker recognized by monoclonal antibody IL-A27, BoT2, and BoT4 or BoT8, and some cells acquired a low level of expression of BoT4, BoT8, or the null cell marker recognized by monoclonal antibody IL-A29. T. parva-transformed null cells retained expression of the IL-A29 determinant and acquired expression of BoT2 and BoT8 but not the IL-A27 determinant or BoT4. T.parva-transformed B cells in most instances lost expression of surface immunoglobulin and never acquired expression of the IL-A27 determinant, BoT2, BoT4, or BoT8, although some cells acquired a low level of expression of the null cell marker recognized by monoclonal antibody IL-A29. Further studies on cell lines and clones grown in vitro from populations isolated from T. parva-infected cattle suggested that the majority of the in vivo T. parva-transformed cells were of T-cell origin.

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