Bovine retinal pigment epithelium promotes proliferation of choroidal endothelium in vitro

Lawrence S Morse, J. Terrell, Y. Sidikaro

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

The invasion of Bruch's membrane and subsequently the subpigment epithelium and subretinal spaces by proliferating choroidal capillaries is a prominent feature of age-related macular degeneration. The ability to isolate and propagate pure cultures of bovine choroidal microvascular endothelium, choroidal pericytes, turbinate fibroblasts, and bovine retinal pigment epithelium allowed us to identify a growth-promoting activity produced by the retinal pigment epithelium that stimulated the proliferation of these cells in vitro. This mitogenic activity is present in both retinal pigment epithelium-conditioned medium and retinal pigment epithelium extract and enhances the mitogenic activity of retinal-derived extract. The activity is resistant to extremes of temperature and pH but partially sensitive to trypsin inactivation.

Original languageEnglish (US)
Pages (from-to)1659-1663
Number of pages5
JournalArchives of Ophthalmology
Volume107
Issue number11
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology

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