Bovine respiratory syncytial virus-specific IgE is associated with interleukin-2 and -4, and interferon-γ expression in pulmonary lymph of experimentally infected calves

Laurel J Gershwin, Robert A. Gunther, Mark L Anderson, Amelia R. Woolums, Kerrie McArthur-Vaughan, K. Erin Randel, Gabrielle A. Boyle, Kathleen E. Friebertshauser, Pat S. McInturff

Research output: Contribution to journalArticle

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Abstract

Objective - To study the local immune response of calves to bovine respiratory syncytial virus (BRSV) infection with emphasis on IgE production and cytokine gene expression in pulmonary lymph. Animals - Twelve 6-to 8-week-old Holstein bull calves. Six similar control calves were mock infected to obtain control data. Procedure - Lymphatic cannulation surgery was performed on 12 calves to create a long-term thoracic lymph fistula draining to the exterior. Cannulated calves were exposed to virulent BRSV by aerosol. Lymph fluid collected daily was assayed for BRSV and isotype-specific IgE antibody, total IgG, IgA, IgM, and protein concentrations, Interleukin-4 (IL-4), interieukin-2 (IL-2), and interferon-γ were semi-quantitated by reverse transcription-polymerase chain reaction (RT-PCR). Cell counts and fluorescence-activated cell scanner (FACSCAN) analysis of T-cell subsets were performed on lymph cells. Results - Calves had clinical signs of respiratory tract disease during days 5 to 10 after infection and shed virus. Bovine respiratory syncytial virus-specific IgE in infected calves was significantly increased over baseline on day 9 after infection. Mean virus-specific IgE concentrations strongly correlated with increases in severity of clinical disease (r = 0.903). Expression of IL-2, IL-4, and interferon-γ was variably present in infected and control calves, with IL-4 expression most consistent during early infection. Conclusions and Clinical Relevance - Infection with BRSV was associated with production of BRSV-specific IgE, and IL-4 message was commonly found in lymph cells of infected calves. This finding supports the concept that BRSV-induced pathophysiology involves a T helper cell type-2 response. Effective therapeutic and prophylactic strategies could, therefore, be developed using immunomodulation to shift the immune response more toward a T helper cell type-1 response.

Original languageEnglish (US)
Pages (from-to)291-298
Number of pages8
JournalAmerican Journal of Veterinary Research
Volume61
Issue number3
StatePublished - Mar 2000

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Bovine respiratory syncytial virus
lymph
Lymph
interferons
interleukin-2
Interleukin-4
Interferons
Immunoglobulin E
Interleukin-2
lungs
calves
Lung
interleukin-4
infection
T-lymphocytes
Infection
Respiratory Tract Diseases
Respiratory Syncytial Virus Infections
Th2 Cells
Th1 Cells

ASJC Scopus subject areas

  • veterinary(all)

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Bovine respiratory syncytial virus-specific IgE is associated with interleukin-2 and -4, and interferon-γ expression in pulmonary lymph of experimentally infected calves. / Gershwin, Laurel J; Gunther, Robert A.; Anderson, Mark L; Woolums, Amelia R.; McArthur-Vaughan, Kerrie; Randel, K. Erin; Boyle, Gabrielle A.; Friebertshauser, Kathleen E.; McInturff, Pat S.

In: American Journal of Veterinary Research, Vol. 61, No. 3, 03.2000, p. 291-298.

Research output: Contribution to journalArticle

Gershwin, Laurel J ; Gunther, Robert A. ; Anderson, Mark L ; Woolums, Amelia R. ; McArthur-Vaughan, Kerrie ; Randel, K. Erin ; Boyle, Gabrielle A. ; Friebertshauser, Kathleen E. ; McInturff, Pat S. / Bovine respiratory syncytial virus-specific IgE is associated with interleukin-2 and -4, and interferon-γ expression in pulmonary lymph of experimentally infected calves. In: American Journal of Veterinary Research. 2000 ; Vol. 61, No. 3. pp. 291-298.
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abstract = "Objective - To study the local immune response of calves to bovine respiratory syncytial virus (BRSV) infection with emphasis on IgE production and cytokine gene expression in pulmonary lymph. Animals - Twelve 6-to 8-week-old Holstein bull calves. Six similar control calves were mock infected to obtain control data. Procedure - Lymphatic cannulation surgery was performed on 12 calves to create a long-term thoracic lymph fistula draining to the exterior. Cannulated calves were exposed to virulent BRSV by aerosol. Lymph fluid collected daily was assayed for BRSV and isotype-specific IgE antibody, total IgG, IgA, IgM, and protein concentrations, Interleukin-4 (IL-4), interieukin-2 (IL-2), and interferon-γ were semi-quantitated by reverse transcription-polymerase chain reaction (RT-PCR). Cell counts and fluorescence-activated cell scanner (FACSCAN) analysis of T-cell subsets were performed on lymph cells. Results - Calves had clinical signs of respiratory tract disease during days 5 to 10 after infection and shed virus. Bovine respiratory syncytial virus-specific IgE in infected calves was significantly increased over baseline on day 9 after infection. Mean virus-specific IgE concentrations strongly correlated with increases in severity of clinical disease (r = 0.903). Expression of IL-2, IL-4, and interferon-γ was variably present in infected and control calves, with IL-4 expression most consistent during early infection. Conclusions and Clinical Relevance - Infection with BRSV was associated with production of BRSV-specific IgE, and IL-4 message was commonly found in lymph cells of infected calves. This finding supports the concept that BRSV-induced pathophysiology involves a T helper cell type-2 response. Effective therapeutic and prophylactic strategies could, therefore, be developed using immunomodulation to shift the immune response more toward a T helper cell type-1 response.",
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AU - Gershwin, Laurel J

AU - Gunther, Robert A.

AU - Anderson, Mark L

AU - Woolums, Amelia R.

AU - McArthur-Vaughan, Kerrie

AU - Randel, K. Erin

AU - Boyle, Gabrielle A.

AU - Friebertshauser, Kathleen E.

AU - McInturff, Pat S.

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N2 - Objective - To study the local immune response of calves to bovine respiratory syncytial virus (BRSV) infection with emphasis on IgE production and cytokine gene expression in pulmonary lymph. Animals - Twelve 6-to 8-week-old Holstein bull calves. Six similar control calves were mock infected to obtain control data. Procedure - Lymphatic cannulation surgery was performed on 12 calves to create a long-term thoracic lymph fistula draining to the exterior. Cannulated calves were exposed to virulent BRSV by aerosol. Lymph fluid collected daily was assayed for BRSV and isotype-specific IgE antibody, total IgG, IgA, IgM, and protein concentrations, Interleukin-4 (IL-4), interieukin-2 (IL-2), and interferon-γ were semi-quantitated by reverse transcription-polymerase chain reaction (RT-PCR). Cell counts and fluorescence-activated cell scanner (FACSCAN) analysis of T-cell subsets were performed on lymph cells. Results - Calves had clinical signs of respiratory tract disease during days 5 to 10 after infection and shed virus. Bovine respiratory syncytial virus-specific IgE in infected calves was significantly increased over baseline on day 9 after infection. Mean virus-specific IgE concentrations strongly correlated with increases in severity of clinical disease (r = 0.903). Expression of IL-2, IL-4, and interferon-γ was variably present in infected and control calves, with IL-4 expression most consistent during early infection. Conclusions and Clinical Relevance - Infection with BRSV was associated with production of BRSV-specific IgE, and IL-4 message was commonly found in lymph cells of infected calves. This finding supports the concept that BRSV-induced pathophysiology involves a T helper cell type-2 response. Effective therapeutic and prophylactic strategies could, therefore, be developed using immunomodulation to shift the immune response more toward a T helper cell type-1 response.

AB - Objective - To study the local immune response of calves to bovine respiratory syncytial virus (BRSV) infection with emphasis on IgE production and cytokine gene expression in pulmonary lymph. Animals - Twelve 6-to 8-week-old Holstein bull calves. Six similar control calves were mock infected to obtain control data. Procedure - Lymphatic cannulation surgery was performed on 12 calves to create a long-term thoracic lymph fistula draining to the exterior. Cannulated calves were exposed to virulent BRSV by aerosol. Lymph fluid collected daily was assayed for BRSV and isotype-specific IgE antibody, total IgG, IgA, IgM, and protein concentrations, Interleukin-4 (IL-4), interieukin-2 (IL-2), and interferon-γ were semi-quantitated by reverse transcription-polymerase chain reaction (RT-PCR). Cell counts and fluorescence-activated cell scanner (FACSCAN) analysis of T-cell subsets were performed on lymph cells. Results - Calves had clinical signs of respiratory tract disease during days 5 to 10 after infection and shed virus. Bovine respiratory syncytial virus-specific IgE in infected calves was significantly increased over baseline on day 9 after infection. Mean virus-specific IgE concentrations strongly correlated with increases in severity of clinical disease (r = 0.903). Expression of IL-2, IL-4, and interferon-γ was variably present in infected and control calves, with IL-4 expression most consistent during early infection. Conclusions and Clinical Relevance - Infection with BRSV was associated with production of BRSV-specific IgE, and IL-4 message was commonly found in lymph cells of infected calves. This finding supports the concept that BRSV-induced pathophysiology involves a T helper cell type-2 response. Effective therapeutic and prophylactic strategies could, therefore, be developed using immunomodulation to shift the immune response more toward a T helper cell type-1 response.

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