Bovine respiratory syncytial virus and Histophilus somni interaction at the alveolar barrier

J. T. Agnes, B. Zekarias, M. Shao, Mark L Anderson, Laurel J Gershwin, L. B. Corbeil

Research output: Contribution to journalArticle

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Abstract

Our previous studies showed that Histophilus somni and bovine respiratory syncytial virus (BRSV) act synergistically in vivo to cause more severe bovine respiratory disease than either agent alone causes. Since H. somni surface and secreted immunoglobulin binding protein A (IbpA) causes retraction of bovine alveolar type 2 (BAT2) cells and invasion between BAT2 cells in vitro, we investigated mechanisms of BRSV-plus-H. somni infection at the alveolar barrier. BRSV treatment of BAT2 cells prior to treatment with IbpA-rich H. somni concentrated culture supernatant (CCS) resulted in increased BAT2 cell rounding and retraction compared to those with either treatment alone. This mimicked the increased alveolar cell thickening in calves experimentally infected with BRSV followed by H. somni compared to that in calves infected with BRSV or H. somni alone. BRSVplus-H. somni CCS treatment of BAT2 cells also enhanced paracellular migration. The effect of matrix metalloproteinases (MMPs) was investigated as well because microarray analysis revealed that treatment with BRSV plus H. somni synergistically upregulated BAT2 cell expression of mmp1 and mmp3 compared to that in cells treated with either agent alone. Enzyme-linked immunosorbent assay (ELISA) confirmed that MMP1 and MMP3 protein levels were similarly upregulated. In collagen I and collagen IV (targets for MMP1 and MMP3, respectively) substrate zymography, digestion was increased with supernatants from dually treated BAT2 cells compared with those from singly treated cells. Enhanced breakdown of collagen IV in the basal lamina and of fibrillar collagen I in the adjacent interstitium in the dual infection may facilitate dissemination of H. somni infection.

Original languageEnglish (US)
Pages (from-to)2592-2597
Number of pages6
JournalInfection and Immunity
Volume81
Issue number7
DOIs
StatePublished - Jul 2013

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Pasteurellaceae
Bovine respiratory syncytial virus
Alveolar Epithelial Cells
Collagen
Staphylococcal Protein A
Carrier Proteins
Infection
Fibrillar Collagens
Cattle Diseases
Therapeutics
B-Cell Antigen Receptors
Microarray Analysis
Matrix Metalloproteinases
Basement Membrane
Immunoglobulins
Digestion
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

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Bovine respiratory syncytial virus and Histophilus somni interaction at the alveolar barrier. / Agnes, J. T.; Zekarias, B.; Shao, M.; Anderson, Mark L; Gershwin, Laurel J; Corbeil, L. B.

In: Infection and Immunity, Vol. 81, No. 7, 07.2013, p. 2592-2597.

Research output: Contribution to journalArticle

Agnes, J. T. ; Zekarias, B. ; Shao, M. ; Anderson, Mark L ; Gershwin, Laurel J ; Corbeil, L. B. / Bovine respiratory syncytial virus and Histophilus somni interaction at the alveolar barrier. In: Infection and Immunity. 2013 ; Vol. 81, No. 7. pp. 2592-2597.
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