Bovine papillomavirus DNA in neoplastic and nonneoplastic tissues obtained from horses with and without sarcoids in the western United States

Elizabeth A. Carr, Alain P Theon, Bruce R. Madewell, Stephen M Griffey, Margaret E. Hitchcock

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Abstract

Objective-To determine the incidence of bovine papillomavirus (BPV) type 1 or 2 in sarcoids and other samples of cutaneous tissues collected from horses in the western United States. Animals-55 horses with sarcoids and 12 horses without sarcoids. Procedure-Tissue samples (tumor and normal skin from horses with sarcoids and normal skin, papillomas, and nonsarcoid cutaneous neoplasms from horses without sarcoids) were collected. Tissue samples were analyzed for BPV-1 or -2 DNA, using a polymerase chain reaction (PCR) and restriction fragment length polymorphism. The PCR products from 7 sarcoid-affected horses were sequenced to evaluate percentage homology with expected sequences for BPV-1 or -2. Results-Most (94/96, 98%) sarcoids contained BPV DNA. Sixty-two percent of the tumors examined had restriction enzyme patterns consistent with BPV-2. Thirty-one of 49 (63%) samples of normal skin obtained from horses with sarcoids contained BPV DNA. All samples subsequently sequenced had 100% homology with the expected sequences for the specific viral type. All tissues from healthy horses, nonsarcoid neoplasms, and papillomas were negative for BPV DNA. Conclusions and Clinical Relevance-Bovine papillomaviral DNA was detected in essentially all sarcoids examined. There appears to be regional variation in the prevalence of viral types in these tumors. The fact that we detected viral DNA in normal skin samples from horses with sarcoids suggests the possibility of a latent viral phase. Viral latency may be 1 explanation for the high rate of recurrence following surgical excision of sarcoids. (Am J Vet Res 2001;62:741-744).

Original languageEnglish (US)
Pages (from-to)741-744
Number of pages4
JournalAmerican Journal of Veterinary Research
Volume62
Issue number5
StatePublished - May 2001

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Bovine papillomavirus
sarcoid
Western United States
Horses
Bovine papillomavirus 1
horses
DNA
skin (animal)
Skin
neoplasms
Papilloma
papilloma
Neoplasms
sampling
Virus Latency
tissues
Polymerase Chain Reaction
polymerase chain reaction
Viral DNA
Skin Neoplasms

ASJC Scopus subject areas

  • veterinary(all)

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Bovine papillomavirus DNA in neoplastic and nonneoplastic tissues obtained from horses with and without sarcoids in the western United States. / Carr, Elizabeth A.; Theon, Alain P; Madewell, Bruce R.; Griffey, Stephen M; Hitchcock, Margaret E.

In: American Journal of Veterinary Research, Vol. 62, No. 5, 05.2001, p. 741-744.

Research output: Contribution to journalArticle

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abstract = "Objective-To determine the incidence of bovine papillomavirus (BPV) type 1 or 2 in sarcoids and other samples of cutaneous tissues collected from horses in the western United States. Animals-55 horses with sarcoids and 12 horses without sarcoids. Procedure-Tissue samples (tumor and normal skin from horses with sarcoids and normal skin, papillomas, and nonsarcoid cutaneous neoplasms from horses without sarcoids) were collected. Tissue samples were analyzed for BPV-1 or -2 DNA, using a polymerase chain reaction (PCR) and restriction fragment length polymorphism. The PCR products from 7 sarcoid-affected horses were sequenced to evaluate percentage homology with expected sequences for BPV-1 or -2. Results-Most (94/96, 98{\%}) sarcoids contained BPV DNA. Sixty-two percent of the tumors examined had restriction enzyme patterns consistent with BPV-2. Thirty-one of 49 (63{\%}) samples of normal skin obtained from horses with sarcoids contained BPV DNA. All samples subsequently sequenced had 100{\%} homology with the expected sequences for the specific viral type. All tissues from healthy horses, nonsarcoid neoplasms, and papillomas were negative for BPV DNA. Conclusions and Clinical Relevance-Bovine papillomaviral DNA was detected in essentially all sarcoids examined. There appears to be regional variation in the prevalence of viral types in these tumors. The fact that we detected viral DNA in normal skin samples from horses with sarcoids suggests the possibility of a latent viral phase. Viral latency may be 1 explanation for the high rate of recurrence following surgical excision of sarcoids. (Am J Vet Res 2001;62:741-744).",
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AB - Objective-To determine the incidence of bovine papillomavirus (BPV) type 1 or 2 in sarcoids and other samples of cutaneous tissues collected from horses in the western United States. Animals-55 horses with sarcoids and 12 horses without sarcoids. Procedure-Tissue samples (tumor and normal skin from horses with sarcoids and normal skin, papillomas, and nonsarcoid cutaneous neoplasms from horses without sarcoids) were collected. Tissue samples were analyzed for BPV-1 or -2 DNA, using a polymerase chain reaction (PCR) and restriction fragment length polymorphism. The PCR products from 7 sarcoid-affected horses were sequenced to evaluate percentage homology with expected sequences for BPV-1 or -2. Results-Most (94/96, 98%) sarcoids contained BPV DNA. Sixty-two percent of the tumors examined had restriction enzyme patterns consistent with BPV-2. Thirty-one of 49 (63%) samples of normal skin obtained from horses with sarcoids contained BPV DNA. All samples subsequently sequenced had 100% homology with the expected sequences for the specific viral type. All tissues from healthy horses, nonsarcoid neoplasms, and papillomas were negative for BPV DNA. Conclusions and Clinical Relevance-Bovine papillomaviral DNA was detected in essentially all sarcoids examined. There appears to be regional variation in the prevalence of viral types in these tumors. The fact that we detected viral DNA in normal skin samples from horses with sarcoids suggests the possibility of a latent viral phase. Viral latency may be 1 explanation for the high rate of recurrence following surgical excision of sarcoids. (Am J Vet Res 2001;62:741-744).

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