Bovine osteopontin modifies the intestinal transcriptome of formula-fed infant rhesus monkeys to be more similar to those that were breastfed

Sharon M. Donovan, Marcia H. Monaco, Jenny Drnevich, Anne Staudt Kvistgaard, Olle Hernell, Bo Lönnerdal

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Osteopontin (OPN) is a multifunctional protein found in human milk at high concentration. Objective: The impact of supplemental bovine OPN on growth, body composition, and the jejunal transcriptome was assessed. Methods: Newborn rhesus monkeys were randomly assigned to be breastfed (n = 4) or to receive formula [formula fed (FF), n = 6] or formula supplemented with 125 mg/L of bovine OPN (bOPN, n = 6) for 3 mo. Jejunal mRNA was extracted and subjected to microarray analysis. Results: Growth was similar among all the treatment groups, but breastfed monkeys were;25% leaner at 3 mo. Pairwise comparisons demonstrated that 1017 genes were differentially expressed between breastfed and FF groups, 217 between breastfed and bOPN groups, and 119 between FF and bOPN groups. The data were also analyzed with the use of weighted gene coexpression network analysis, which revealed 6 modules of coexpressed genes that differed among the 3 treatments. Nearly 50% of genes were assigned to one module in which breastfed differed from FF and bOPN expression was intermediate. This module was enriched for genes related to cell adhesion and motility, cytoskeletal remodeling, wingless and integration site signaling, and neuronal development. Most of these canonical pathways centered on integrins, which are receptors for OPN. Conclusions: The intestinal transcriptome of breastfed and FF monkeys differs, but bovine OPN at levels similar to human milk shifts gene expression profiles to be more similar to breastfed monkeys.

Original languageEnglish (US)
Pages (from-to)1910-1919
Number of pages10
JournalJournal of Nutrition
Volume144
Issue number12
DOIs
StatePublished - 2014

Fingerprint

Infant Formula
Osteopontin
Macaca mulatta
Transcriptome
Haplorhini
Gene Regulatory Networks
Human Milk
Genes
Microarray Analysis
Growth
Body Composition
Cell Adhesion
Integrins
Cell Movement
Messenger RNA
Proteins

Keywords

  • Breastfeeding
  • Infant
  • Intestine
  • Micorarray
  • Osteopontin

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Medicine(all)

Cite this

Bovine osteopontin modifies the intestinal transcriptome of formula-fed infant rhesus monkeys to be more similar to those that were breastfed. / Donovan, Sharon M.; Monaco, Marcia H.; Drnevich, Jenny; Kvistgaard, Anne Staudt; Hernell, Olle; Lönnerdal, Bo.

In: Journal of Nutrition, Vol. 144, No. 12, 2014, p. 1910-1919.

Research output: Contribution to journalArticle

Donovan, Sharon M. ; Monaco, Marcia H. ; Drnevich, Jenny ; Kvistgaard, Anne Staudt ; Hernell, Olle ; Lönnerdal, Bo. / Bovine osteopontin modifies the intestinal transcriptome of formula-fed infant rhesus monkeys to be more similar to those that were breastfed. In: Journal of Nutrition. 2014 ; Vol. 144, No. 12. pp. 1910-1919.
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abstract = "Background: Osteopontin (OPN) is a multifunctional protein found in human milk at high concentration. Objective: The impact of supplemental bovine OPN on growth, body composition, and the jejunal transcriptome was assessed. Methods: Newborn rhesus monkeys were randomly assigned to be breastfed (n = 4) or to receive formula [formula fed (FF), n = 6] or formula supplemented with 125 mg/L of bovine OPN (bOPN, n = 6) for 3 mo. Jejunal mRNA was extracted and subjected to microarray analysis. Results: Growth was similar among all the treatment groups, but breastfed monkeys were;25{\%} leaner at 3 mo. Pairwise comparisons demonstrated that 1017 genes were differentially expressed between breastfed and FF groups, 217 between breastfed and bOPN groups, and 119 between FF and bOPN groups. The data were also analyzed with the use of weighted gene coexpression network analysis, which revealed 6 modules of coexpressed genes that differed among the 3 treatments. Nearly 50{\%} of genes were assigned to one module in which breastfed differed from FF and bOPN expression was intermediate. This module was enriched for genes related to cell adhesion and motility, cytoskeletal remodeling, wingless and integration site signaling, and neuronal development. Most of these canonical pathways centered on integrins, which are receptors for OPN. Conclusions: The intestinal transcriptome of breastfed and FF monkeys differs, but bovine OPN at levels similar to human milk shifts gene expression profiles to be more similar to breastfed monkeys.",
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