Abstract
Fetal cells and DNA have been detected in the maternal circulation during and after pregnancy in a few mammalian species. The incidence of similar microchimerism in cattle could have repercussion for the application of modern biotechnologies such as the transfer of transgenic embryos. To determine if feto-maternal leakage can occur in pregnant cows, we have analyzed maternal blood samples for the presence of fetal DNA during gestation and post-partum periods. Y chromosome-specific DNA was detected in up to 73% of blood samples from naturally mated heifers carrying conventional bull calves and a transgene-specific sequence in up to 50% of recipient cows carrying transgenic fetuses. These findings document for the first time that transplacental leakage of fetal DNA into the maternal circulation can occur in cattle despite the epitheliochorial placenta of ruminants, with potential implications for the utilization of recipient cows in the food chain.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 486-491 |
| Number of pages | 6 |
| Journal | Biotechnology Journal |
| Volume | 2 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 2007 |
| Externally published | Yes |
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Keywords
- Bovine
- Embryo transfer
- Microchimerism
- Real-time PCR
- Transplacental leakage
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Molecular Medicine
Cite this
Bovine fetal microchimerism in normal and embryo transfer pregnancies and its implications for biotechnology applications in cattle. / Turin, Lauretta; Invernizzi, Pietro; Woodcock, Marion; Grati, Francesca R.; Riva, Federica; Tribbioli, Giulia; Laible, Götz.
In: Biotechnology Journal, Vol. 2, No. 4, 04.2007, p. 486-491.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bovine fetal microchimerism in normal and embryo transfer pregnancies and its implications for biotechnology applications in cattle
AU - Turin, Lauretta
AU - Invernizzi, Pietro
AU - Woodcock, Marion
AU - Grati, Francesca R.
AU - Riva, Federica
AU - Tribbioli, Giulia
AU - Laible, Götz
PY - 2007/4
Y1 - 2007/4
N2 - Fetal cells and DNA have been detected in the maternal circulation during and after pregnancy in a few mammalian species. The incidence of similar microchimerism in cattle could have repercussion for the application of modern biotechnologies such as the transfer of transgenic embryos. To determine if feto-maternal leakage can occur in pregnant cows, we have analyzed maternal blood samples for the presence of fetal DNA during gestation and post-partum periods. Y chromosome-specific DNA was detected in up to 73% of blood samples from naturally mated heifers carrying conventional bull calves and a transgene-specific sequence in up to 50% of recipient cows carrying transgenic fetuses. These findings document for the first time that transplacental leakage of fetal DNA into the maternal circulation can occur in cattle despite the epitheliochorial placenta of ruminants, with potential implications for the utilization of recipient cows in the food chain.
AB - Fetal cells and DNA have been detected in the maternal circulation during and after pregnancy in a few mammalian species. The incidence of similar microchimerism in cattle could have repercussion for the application of modern biotechnologies such as the transfer of transgenic embryos. To determine if feto-maternal leakage can occur in pregnant cows, we have analyzed maternal blood samples for the presence of fetal DNA during gestation and post-partum periods. Y chromosome-specific DNA was detected in up to 73% of blood samples from naturally mated heifers carrying conventional bull calves and a transgene-specific sequence in up to 50% of recipient cows carrying transgenic fetuses. These findings document for the first time that transplacental leakage of fetal DNA into the maternal circulation can occur in cattle despite the epitheliochorial placenta of ruminants, with potential implications for the utilization of recipient cows in the food chain.
KW - Bovine
KW - Embryo transfer
KW - Microchimerism
KW - Real-time PCR
KW - Transplacental leakage
UR - http://www.scopus.com/inward/record.url?scp=34248381681&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34248381681&partnerID=8YFLogxK
U2 - 10.1002/biot.200600218
DO - 10.1002/biot.200600218
M3 - Article
C2 - 17285678
AN - SCOPUS:34248381681
VL - 2
SP - 486
EP - 491
JO - Biotechnology Journal
JF - Biotechnology Journal
SN - 1860-6768
IS - 4
ER -