Both homo and heterodimers of Marek's disease virus encoded Meq protein contribute to transformation of lymphocytes in chickens

Paulette F. Suchodolski, Yoshihiro Izumiya, Blanca Lupiani, Dharani K. Ajithdoss, Lucy F. Lee, Hsing-Jien Kung, Sanjay M. Reddy

Research output: Contribution to journalArticle

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Abstract

Marek' disease virus serotype-1, also know as Gallid herpesvirus 2 (GaHV-2), elicits T-cell lymphomas in chickens. The GaHV-2 genome encodes an oncoprotein, Meq, with similarity to the Jun/Fos family of proteins. We have previously shown that Meq homodimers are not sufficient to induce lymphomas in chickens. In this study, we investigated the role of Meq heterodimers in the pathogenicity of GaHV-2 by generating a chimeric meq gene, which contains the leucine zipper region of Fos (meqFos). A recombinant virus containing the meqFos gene in place of parental meq, rMd5-MeqFos, was not capable of transforming chicken lymphocytes, indicating that heterodimerization of Meq alone is not sufficient for transformation. In addition, the recovery of the oncogenic phenotype by a recombinant virus encoding one copy each of MeqGCN (homodimer) and MeqFos (heterodimer) conclusively demonstrates that both homo and heterodimerization of Meq are required for oncogenesis.

Original languageEnglish (US)
Pages (from-to)312-321
Number of pages10
JournalVirology
Volume399
Issue number2
DOIs
StatePublished - Apr 10 2010

Fingerprint

Gallid Herpesvirus 2
Marek Disease
Lymphocyte Activation
Chickens
Viruses
Proteins
Leucine Zippers
T-Cell Lymphoma
Oncogene Proteins
Genes
Virulence
Lymphoma
Carcinogenesis
Genome
Lymphocytes
Phenotype

Keywords

  • bZIP
  • Chicken
  • Dimerization
  • GaHV-2
  • Herpesvirus
  • Leucine zipper
  • Marek's disease virus
  • Meq
  • Oncogenesis
  • T-cell lymphomas

ASJC Scopus subject areas

  • Virology

Cite this

Both homo and heterodimers of Marek's disease virus encoded Meq protein contribute to transformation of lymphocytes in chickens. / Suchodolski, Paulette F.; Izumiya, Yoshihiro; Lupiani, Blanca; Ajithdoss, Dharani K.; Lee, Lucy F.; Kung, Hsing-Jien; Reddy, Sanjay M.

In: Virology, Vol. 399, No. 2, 10.04.2010, p. 312-321.

Research output: Contribution to journalArticle

Suchodolski, Paulette F. ; Izumiya, Yoshihiro ; Lupiani, Blanca ; Ajithdoss, Dharani K. ; Lee, Lucy F. ; Kung, Hsing-Jien ; Reddy, Sanjay M. / Both homo and heterodimers of Marek's disease virus encoded Meq protein contribute to transformation of lymphocytes in chickens. In: Virology. 2010 ; Vol. 399, No. 2. pp. 312-321.
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