Both B-1a and B-1b cells exposed to Mycobacterium tuberculosis lipids differentiate into IgM antibody-secreting cells

Ciara Ordoñez, Hannah P. Savage, Musharaf Tarajia, René Rivera, Cheyenne Weeks-Galindo, Dilcia Sambrano, Lee Riley, Patricia L. Fernandez, Nicole Baumgarth, Amador Goodridge

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. The cellular immune response to mycobacteria has been characterized extensively, but the antibody response remains underexplored. The present study aimed to examine whether host or bacterial phospholipids induce secretion of IgM, and specifically anti-phospholipid IgM, antibodies by B cells and to identify the responsible B-cell subset. Here we show that peritoneal B cells responded to lipid antigens by secreting IgM antibodies. Specifically, stimulation with M. tuberculosis H37Rv total lipids resulted in significant induction of total and anti-phosphatidylcholine IgM. Similarly, IgM antibody production increased significantly with stimulation by whole Mycobacterium bovis bacillus Calmette-Guérin. The B-1 subset was the dominant source of IgM antibodies after exposure to cardiolipin. Both CD5+ B-1a and CD5- B-1b cell subsets secreted total IgM antibodies after exposure to M. tuberculosis H37Rv total lipids in vitro. Overall, our results suggest that the poly-reactive B-1 cell repertoire contributes to non-specific anti-phospholipid IgM antibody secretion in response to M. tuberculosis lipids.

Original languageEnglish (US)
JournalImmunology
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Antibody-Producing Cells
Mycobacterium tuberculosis
Immunoglobulin M
Lipids
Antibodies
B-Lymphocyte Subsets
Phospholipids
Antibody Formation
B-Lymphocytes
Cardiolipins
Mycobacterium
Mycobacterium bovis
Phosphatidylcholines
Cellular Immunity
Bacillus
Communicable Diseases
Tuberculosis
Antigens
anti-IgM

Keywords

  • Activation
  • Autoantibodies
  • B cells
  • Bacterial
  • Innate lymphoid cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Ordoñez, C., Savage, H. P., Tarajia, M., Rivera, R., Weeks-Galindo, C., Sambrano, D., ... Goodridge, A. (Accepted/In press). Both B-1a and B-1b cells exposed to Mycobacterium tuberculosis lipids differentiate into IgM antibody-secreting cells. Immunology. https://doi.org/10.1111/imm.12909

Both B-1a and B-1b cells exposed to Mycobacterium tuberculosis lipids differentiate into IgM antibody-secreting cells. / Ordoñez, Ciara; Savage, Hannah P.; Tarajia, Musharaf; Rivera, René; Weeks-Galindo, Cheyenne; Sambrano, Dilcia; Riley, Lee; Fernandez, Patricia L.; Baumgarth, Nicole; Goodridge, Amador.

In: Immunology, 01.01.2018.

Research output: Contribution to journalArticle

Ordoñez, C, Savage, HP, Tarajia, M, Rivera, R, Weeks-Galindo, C, Sambrano, D, Riley, L, Fernandez, PL, Baumgarth, N & Goodridge, A 2018, 'Both B-1a and B-1b cells exposed to Mycobacterium tuberculosis lipids differentiate into IgM antibody-secreting cells', Immunology. https://doi.org/10.1111/imm.12909
Ordoñez, Ciara ; Savage, Hannah P. ; Tarajia, Musharaf ; Rivera, René ; Weeks-Galindo, Cheyenne ; Sambrano, Dilcia ; Riley, Lee ; Fernandez, Patricia L. ; Baumgarth, Nicole ; Goodridge, Amador. / Both B-1a and B-1b cells exposed to Mycobacterium tuberculosis lipids differentiate into IgM antibody-secreting cells. In: Immunology. 2018.
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