Borrelia burgdorferi strain-specific Osp C-mediated immunity in mice

Linda K. Bockenstedt, Emir Hodzic, Sunlian Feng, Ken W. Bourrel, Aravinda De Silva, Ruth R. Montgomery, Erol Fikrig, Justin D. Radolf, Stephen W Barthold

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Antibodies to the outer surface proteins (Osps) A, B, and C of the spirochete Borrelia burgdorferi can prevent infection in animal models of Lyme borreliosis. We have previously demonstrated that immune serum from mice infected with B. burgdorferi N40 can also prevent challenge infection and induce disease regression in infected mice. The antigens targeted by protective and disease-modulating antibodies are presently unknown, but they do not include Osp A or Osp B. Because Osp C antibodies are present in immune mouse serum, we investigated the ability of hyperimmune serum to recombinant Osp C (N40) to protect mice against challenge infection with N40 spirochetes. In both active and passive immunization studies, Osp C (N40) antiserum failed to protect mice from challenge infection with cultured organisms. Mice actively immunized with recombinant Osp C (N40) were susceptible to tick- borne challenge infection, and nymphal ticks remained infected after feeding on Osp C-hyperimmunized mice. In contrast, similar immunization studies performed with Osp C (PKo) antiserum prevented challenge infection of mice with a clone of PKo spirochetes pathogenic for mice. Both Osp C (N40) and Osp C (PKo) antisera showed minimal in vitro borreliacidal activity, and immunofluorescence studies localized Osp C beneath the outer membrane of both N40 and PKo spirochetes. We conclude that Osp C antibody-mediated immunity is strain specific and propose float differences in Osp C surface expression by spirochetes in vivo may account for strain-specific immunity.

Original languageEnglish (US)
Pages (from-to)4661-4667
Number of pages7
JournalInfection and Immunity
Volume65
Issue number11
StatePublished - 1997
Externally publishedYes

Fingerprint

Borrelia burgdorferi
Immunity
Spirochaetales
Immune Sera
Infection
Antibodies
Tick-Borne Diseases
Passive Immunization
Lyme Disease
Ticks
Fluorescent Antibody Technique
Immunization
Vaccination
Animal Models
Clone Cells
Antigens
Membranes

ASJC Scopus subject areas

  • Immunology

Cite this

Bockenstedt, L. K., Hodzic, E., Feng, S., Bourrel, K. W., De Silva, A., Montgomery, R. R., ... Barthold, S. W. (1997). Borrelia burgdorferi strain-specific Osp C-mediated immunity in mice. Infection and Immunity, 65(11), 4661-4667.

Borrelia burgdorferi strain-specific Osp C-mediated immunity in mice. / Bockenstedt, Linda K.; Hodzic, Emir; Feng, Sunlian; Bourrel, Ken W.; De Silva, Aravinda; Montgomery, Ruth R.; Fikrig, Erol; Radolf, Justin D.; Barthold, Stephen W.

In: Infection and Immunity, Vol. 65, No. 11, 1997, p. 4661-4667.

Research output: Contribution to journalArticle

Bockenstedt, LK, Hodzic, E, Feng, S, Bourrel, KW, De Silva, A, Montgomery, RR, Fikrig, E, Radolf, JD & Barthold, SW 1997, 'Borrelia burgdorferi strain-specific Osp C-mediated immunity in mice', Infection and Immunity, vol. 65, no. 11, pp. 4661-4667.
Bockenstedt LK, Hodzic E, Feng S, Bourrel KW, De Silva A, Montgomery RR et al. Borrelia burgdorferi strain-specific Osp C-mediated immunity in mice. Infection and Immunity. 1997;65(11):4661-4667.
Bockenstedt, Linda K. ; Hodzic, Emir ; Feng, Sunlian ; Bourrel, Ken W. ; De Silva, Aravinda ; Montgomery, Ruth R. ; Fikrig, Erol ; Radolf, Justin D. ; Barthold, Stephen W. / Borrelia burgdorferi strain-specific Osp C-mediated immunity in mice. In: Infection and Immunity. 1997 ; Vol. 65, No. 11. pp. 4661-4667.
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AU - De Silva, Aravinda

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AU - Fikrig, Erol

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