Borrelia burgdorferi population dynamics and prototype gene expression during infection of immunocompetent and immunodeficient mice

Emir Hodzic, Sunlian Feng, Kim J. Freet, Stephen W Barthold

Research output: Contribution to journalArticle

92 Scopus citations

Abstract

The population dynamics of Borrelia burgdorferi were quantified by real-time PCR targeting the flaB gene in skin (inoculation site, noninoculation site, and ear), heart (heart base and ventricle), quadriceps muscle, and the tibiotarsal joint at 1, 2, 4, 6, and 8 weeks after intradermal inoculation in C3H and C3H-scid mice. In addition, RNA transcription was assessed for several prototype genes, including flaB, ospA, ospC, dbpA, arp, vlsE, fbp, oppA-2, and p37-42. Spirochete numbers were equivalent in C3H and C3H-scid mice at 1 or 2 weeks and then declined in C3H mice, but they continued to rise and then plateaued in C3H-scid mice. Gene transcription was likewise higher in C3H-scid mice than in C3H mice, particularly at 4 or more weeks of infection. Gene transcription showed variation among tissues, with the highest levels of transcription in heart and joint tissue, which are sites of inflammation.

Original languageEnglish (US)
Pages (from-to)5042-5055
Number of pages14
JournalInfection and Immunity
Volume71
Issue number9
DOIs
StatePublished - Sep 1 2003

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ASJC Scopus subject areas

  • Immunology

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