Borrelia burgdorferi population dynamics and prototype gene expression during infection of immunocompetent and immunodeficient mice

Emir Hodzic, Sunlian Feng, Kim J. Freet, Stephen W Barthold

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

The population dynamics of Borrelia burgdorferi were quantified by real-time PCR targeting the flaB gene in skin (inoculation site, noninoculation site, and ear), heart (heart base and ventricle), quadriceps muscle, and the tibiotarsal joint at 1, 2, 4, 6, and 8 weeks after intradermal inoculation in C3H and C3H-scid mice. In addition, RNA transcription was assessed for several prototype genes, including flaB, ospA, ospC, dbpA, arp, vlsE, fbp, oppA-2, and p37-42. Spirochete numbers were equivalent in C3H and C3H-scid mice at 1 or 2 weeks and then declined in C3H mice, but they continued to rise and then plateaued in C3H-scid mice. Gene transcription was likewise higher in C3H-scid mice than in C3H mice, particularly at 4 or more weeks of infection. Gene transcription showed variation among tissues, with the highest levels of transcription in heart and joint tissue, which are sites of inflammation.

Original languageEnglish (US)
Pages (from-to)5042-5055
Number of pages14
JournalInfection and Immunity
Volume71
Issue number9
DOIs
StatePublished - Sep 1 2003

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Borrelia burgdorferi
Inbred C3H Mouse
Population Dynamics
Gene Expression
Infection
Joints
Genes
Spirochaetales
Gene Targeting
Quadriceps Muscle
Heart Ventricles
Ear
Real-Time Polymerase Chain Reaction
RNA
Inflammation
Skin

ASJC Scopus subject areas

  • Immunology

Cite this

Borrelia burgdorferi population dynamics and prototype gene expression during infection of immunocompetent and immunodeficient mice. / Hodzic, Emir; Feng, Sunlian; Freet, Kim J.; Barthold, Stephen W.

In: Infection and Immunity, Vol. 71, No. 9, 01.09.2003, p. 5042-5055.

Research output: Contribution to journalArticle

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