Borrelia burgdorferi P35 and P37 proteins, expressed in vivo, elicit protective immunity

Erol Fikrig, Stephen W Barthold, Wei Sun, Wen Feng, Sam R. Telford, Richard A. Flavell

Research output: Contribution to journalArticle

119 Scopus citations

Abstract

p35 and p37 are Borrelia burgdorferi genes encoding 35 and 31 kDa proteins. The gene products were identified by differential screening of a B. burgdorferi expression library with sera from B. burgdorferi infected and B. burgdorferi-hyperimmunized mice. Northern blot and RT-PCR analyses confirmed that these genes were selectively expressed in vivo. ELISA, using P35 and P37, showed that infected mice (5 of 5, 100%) and patients (31 of 43, 72%) with Lyme borreliosis developed P35 or P37 antibodies. Mice developed peak IgG titers to P35 and P37 within 30 days, followed by decline. Mice given both P35 and P37 antisera were protected from challenge with 102 B. burgdorferi, and P35 and P37 antisera also afforded protection when administered 24 hr after spirochete challenge. The use of in vivo-expressed antigens such as P35 and P37 represents a new approach for Lyme disease serodiagnosis and for understanding the role of B. burgdorferi-specific immune responses in host immunity.

Original languageEnglish (US)
Pages (from-to)531-539
Number of pages9
JournalImmunity
Volume6
Issue number5
DOIs
StatePublished - May 1997
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

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