Borrelia burgdorferi infection and immunity in mice deficient in the fifth component of complement

L. K. Bockenstedt, Stephen W Barthold, K. Deponte, N. Marcantonio, F. S. Kantor

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

When immunocompetent mice are inoculated with Borrelia burgdorferi, they develop acute arthritis and carditis that undergo spontaneous regression despite the persistence of infection. Specific T- and/or B-cell immunity appears to be necessary for resolution of disease manifestations. Humoral immune responses to B. burgdorferi are also important in prevention of B. burgdorferi infection, in that passive transfer of immune sera or protective monoclonal antibodies prevents the spirochete from establishing infection. It has previously been suggested that complement is necessary for effective antibody-mediated host responses against B. burgdorferi. To investigate the role of complement in the pathogenesis and prevention of Lyme disease, we compared the responses to B. burgdorferi challenge inoculation of mice genetically deficient in the fifth component of complement (C5) with those of C5-sufficient mice. All C5-deficient strains tested were susceptible to B. burgdorferi infection, and disease manifestations underwent regression in a similar time-course to those of complement-sufficient mice. Moreover, passive immunization of C5-deficient mice with either immune rabbit sera or neutralizing monoclonal antibody protected them from challenge infection. These results demonstrate that the expression of Lyme disease is not altered in mice deficient in C5 and that C5-mediated complement activation is not necessary for antibody-mediated protection from infection.

Original languageEnglish (US)
Pages (from-to)2104-2107
Number of pages4
JournalInfection and Immunity
Volume61
Issue number5
StatePublished - 1993
Externally publishedYes

Fingerprint

Borrelia Infections
Borrelia burgdorferi
Immunity
Lyme Disease
Immune Sera
Spirochaetales Infections
Infection
Complement C5
Monoclonal Antibodies
Passive Immunization
Antibodies
Complement Activation
Myocarditis
Humoral Immunity
Neutralizing Antibodies
Arthritis
B-Lymphocytes
Rabbits

ASJC Scopus subject areas

  • Immunology

Cite this

Bockenstedt, L. K., Barthold, S. W., Deponte, K., Marcantonio, N., & Kantor, F. S. (1993). Borrelia burgdorferi infection and immunity in mice deficient in the fifth component of complement. Infection and Immunity, 61(5), 2104-2107.

Borrelia burgdorferi infection and immunity in mice deficient in the fifth component of complement. / Bockenstedt, L. K.; Barthold, Stephen W; Deponte, K.; Marcantonio, N.; Kantor, F. S.

In: Infection and Immunity, Vol. 61, No. 5, 1993, p. 2104-2107.

Research output: Contribution to journalArticle

Bockenstedt, LK, Barthold, SW, Deponte, K, Marcantonio, N & Kantor, FS 1993, 'Borrelia burgdorferi infection and immunity in mice deficient in the fifth component of complement', Infection and Immunity, vol. 61, no. 5, pp. 2104-2107.
Bockenstedt, L. K. ; Barthold, Stephen W ; Deponte, K. ; Marcantonio, N. ; Kantor, F. S. / Borrelia burgdorferi infection and immunity in mice deficient in the fifth component of complement. In: Infection and Immunity. 1993 ; Vol. 61, No. 5. pp. 2104-2107.
@article{425efb9d5a9045ac96461298d6a1cc06,
title = "Borrelia burgdorferi infection and immunity in mice deficient in the fifth component of complement",
abstract = "When immunocompetent mice are inoculated with Borrelia burgdorferi, they develop acute arthritis and carditis that undergo spontaneous regression despite the persistence of infection. Specific T- and/or B-cell immunity appears to be necessary for resolution of disease manifestations. Humoral immune responses to B. burgdorferi are also important in prevention of B. burgdorferi infection, in that passive transfer of immune sera or protective monoclonal antibodies prevents the spirochete from establishing infection. It has previously been suggested that complement is necessary for effective antibody-mediated host responses against B. burgdorferi. To investigate the role of complement in the pathogenesis and prevention of Lyme disease, we compared the responses to B. burgdorferi challenge inoculation of mice genetically deficient in the fifth component of complement (C5) with those of C5-sufficient mice. All C5-deficient strains tested were susceptible to B. burgdorferi infection, and disease manifestations underwent regression in a similar time-course to those of complement-sufficient mice. Moreover, passive immunization of C5-deficient mice with either immune rabbit sera or neutralizing monoclonal antibody protected them from challenge infection. These results demonstrate that the expression of Lyme disease is not altered in mice deficient in C5 and that C5-mediated complement activation is not necessary for antibody-mediated protection from infection.",
author = "Bockenstedt, {L. K.} and Barthold, {Stephen W} and K. Deponte and N. Marcantonio and Kantor, {F. S.}",
year = "1993",
language = "English (US)",
volume = "61",
pages = "2104--2107",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "5",

}

TY - JOUR

T1 - Borrelia burgdorferi infection and immunity in mice deficient in the fifth component of complement

AU - Bockenstedt, L. K.

AU - Barthold, Stephen W

AU - Deponte, K.

AU - Marcantonio, N.

AU - Kantor, F. S.

PY - 1993

Y1 - 1993

N2 - When immunocompetent mice are inoculated with Borrelia burgdorferi, they develop acute arthritis and carditis that undergo spontaneous regression despite the persistence of infection. Specific T- and/or B-cell immunity appears to be necessary for resolution of disease manifestations. Humoral immune responses to B. burgdorferi are also important in prevention of B. burgdorferi infection, in that passive transfer of immune sera or protective monoclonal antibodies prevents the spirochete from establishing infection. It has previously been suggested that complement is necessary for effective antibody-mediated host responses against B. burgdorferi. To investigate the role of complement in the pathogenesis and prevention of Lyme disease, we compared the responses to B. burgdorferi challenge inoculation of mice genetically deficient in the fifth component of complement (C5) with those of C5-sufficient mice. All C5-deficient strains tested were susceptible to B. burgdorferi infection, and disease manifestations underwent regression in a similar time-course to those of complement-sufficient mice. Moreover, passive immunization of C5-deficient mice with either immune rabbit sera or neutralizing monoclonal antibody protected them from challenge infection. These results demonstrate that the expression of Lyme disease is not altered in mice deficient in C5 and that C5-mediated complement activation is not necessary for antibody-mediated protection from infection.

AB - When immunocompetent mice are inoculated with Borrelia burgdorferi, they develop acute arthritis and carditis that undergo spontaneous regression despite the persistence of infection. Specific T- and/or B-cell immunity appears to be necessary for resolution of disease manifestations. Humoral immune responses to B. burgdorferi are also important in prevention of B. burgdorferi infection, in that passive transfer of immune sera or protective monoclonal antibodies prevents the spirochete from establishing infection. It has previously been suggested that complement is necessary for effective antibody-mediated host responses against B. burgdorferi. To investigate the role of complement in the pathogenesis and prevention of Lyme disease, we compared the responses to B. burgdorferi challenge inoculation of mice genetically deficient in the fifth component of complement (C5) with those of C5-sufficient mice. All C5-deficient strains tested were susceptible to B. burgdorferi infection, and disease manifestations underwent regression in a similar time-course to those of complement-sufficient mice. Moreover, passive immunization of C5-deficient mice with either immune rabbit sera or neutralizing monoclonal antibody protected them from challenge infection. These results demonstrate that the expression of Lyme disease is not altered in mice deficient in C5 and that C5-mediated complement activation is not necessary for antibody-mediated protection from infection.

UR - http://www.scopus.com/inward/record.url?scp=0027173926&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027173926&partnerID=8YFLogxK

M3 - Article

C2 - 8478100

AN - SCOPUS:0027173926

VL - 61

SP - 2104

EP - 2107

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 5

ER -