Borrelia burgdorferi-induced inflammation facilitates spirochete adaptation and variable major protein-like sequence locus recombination

J. Anguita, V. Thomas, S. Samanta, R. Persinski, C. Hernanz, Stephen W Barthold, E. Fikrig

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Spirochete adaptation in vivo is associated with preferential Borrelia burgdorferi gene expression. In this paper, we show that the administration of B. burgdorferi-immune sera to IFN-γR-deficient mice that have been infected with B. burgdorferi N40 for 4 days causes spirochete clearance. In contrast, immune sera-mediated clearance of B. burgdorferi N40 is not apparent in immunocompetent mice, suggesting a role for IFN-γ-mediated responses in B. burgdorferi N40 host adaptation. B. burgdorferi-immune sera also induces clearance of B. burgdorferi N40 that have been passaged in vitro 75 times (B. burgdorferi N40-75), a derivative of B. burgdorferi N40 that does not rapidly adapt in vivo in immunocompetent mice. B. burgdorferi N40-75 produce lower levels of IFN-γ and IL-12 in mice than does B. burgdorferi N40, and the administration of these cytokines to B. burgdorferi N40-75-infected mice results in an increased spirochetal burden, further indicating that IFN-γ-mediated events promote B. burgdorferi survival. Differential immunoscreening and RT-PCR demonstrate that IFN-γ-mediated signals facilitate spirochete recombination at the variable major protein like sequence locus, a site for early antigenic variation in vivo, and that recombination rates by B. burgdorferi N40 are lower in IFN-γR-deficient mice than in control animals. These results suggest that the murine immune response can promote the in vivo adaptation of B. burgdorferi.

Original languageEnglish (US)
Pages (from-to)3383-3390
Number of pages8
JournalJournal of Immunology
Volume167
Issue number6
StatePublished - Sep 15 2001
Externally publishedYes

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Spirochaetales
Borrelia burgdorferi
Genetic Recombination
Inflammation
Proteins
Immune Sera
Antigenic Variation
Interleukin-12

ASJC Scopus subject areas

  • Immunology

Cite this

Borrelia burgdorferi-induced inflammation facilitates spirochete adaptation and variable major protein-like sequence locus recombination. / Anguita, J.; Thomas, V.; Samanta, S.; Persinski, R.; Hernanz, C.; Barthold, Stephen W; Fikrig, E.

In: Journal of Immunology, Vol. 167, No. 6, 15.09.2001, p. 3383-3390.

Research output: Contribution to journalArticle

Anguita, J, Thomas, V, Samanta, S, Persinski, R, Hernanz, C, Barthold, SW & Fikrig, E 2001, 'Borrelia burgdorferi-induced inflammation facilitates spirochete adaptation and variable major protein-like sequence locus recombination', Journal of Immunology, vol. 167, no. 6, pp. 3383-3390.
Anguita, J. ; Thomas, V. ; Samanta, S. ; Persinski, R. ; Hernanz, C. ; Barthold, Stephen W ; Fikrig, E. / Borrelia burgdorferi-induced inflammation facilitates spirochete adaptation and variable major protein-like sequence locus recombination. In: Journal of Immunology. 2001 ; Vol. 167, No. 6. pp. 3383-3390.
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