Border disease. Virus-induced decrease in thyroid hormone levels with associated hypomyelination

C. A. Anderson, Robert Higgins, M. E. Smith, Bennie Osburn

Research output: Contribution to journalArticle

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Abstract

Border disease (BD) was induced in lambs by inoculation of their dams at 50 days gestation with Border disease virus (BDV) isolate #31. At birth, the clinically affected lambs had diffuse spinal cord hypomyelination, confirmed by immunocytochemical staining for myelin-associated glycoprotein and myelin basic protein. In the BD lambs, large numbers of thyroid follicular epithelial cells and scattered pituitary cells contained BDV antigen by immunofluorescence staining. Double labeling techniques demonstrated the BDV-infected pituitary cells to contain growth hormone, adrenocorticotrophic hormone, prolactin, or luteinizing hormone. Cells containing thyroid stimulating hormone were rare and were not positive for BDV antigen. Infection of the pituitaries and thyroid glands caused no detectable morphologic changes as compared to controls. The BD lambs had statistically significantly (p < 0.05) lower mean serum concentrations of thyroxine and L-3,3',5-triiodothyronine as compared to age-matched uninfected controls. Similar significant differences in the mean plasma levels of growth hormone and thyroid stimulating hormone were not found. In addition, the BD lambs had a statistically significant (p < 0.05) lower mean activity of the myelin-associated, thyroid hormone dependent enzyme, 2',3'-cyclic nucleotide-3'-phosphodiesterase in spinal cord tissue. Although not conclusive, these results indicate that the hypomyelination in BD may be due to depressed levels of circulating thyroid gland hormones secondary to a noninflammatory and noncytolytic infection of the thyroid gland by BDV. This is one of the first reports indicating that a virus-induced hormonal alteration may cause a congenital lesion in animals.

Original languageEnglish (US)
Pages (from-to)168-175
Number of pages8
JournalLaboratory Investigation
Volume57
Issue number2
StatePublished - Jan 1 1987

Fingerprint

Border Disease
Border disease virus
Thyroid Hormones
Viruses
Thyroid Gland
Thyrotropin
Growth Hormone
Spinal Cord
2',3'-Cyclic Nucleotide 3'-Phosphodiesterase
Myelin-Associated Glycoprotein
Reverse Triiodothyronine
Staining and Labeling
Antigens
Myelin Basic Protein
Pituitary Gland
Myelin Sheath
Luteinizing Hormone
Infection
Thyroxine
Prolactin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Border disease. Virus-induced decrease in thyroid hormone levels with associated hypomyelination. / Anderson, C. A.; Higgins, Robert; Smith, M. E.; Osburn, Bennie.

In: Laboratory Investigation, Vol. 57, No. 2, 01.01.1987, p. 168-175.

Research output: Contribution to journalArticle

Anderson, C. A. ; Higgins, Robert ; Smith, M. E. ; Osburn, Bennie. / Border disease. Virus-induced decrease in thyroid hormone levels with associated hypomyelination. In: Laboratory Investigation. 1987 ; Vol. 57, No. 2. pp. 168-175.
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