Border disease: tissue culture studies of the virus in sheep.

B. J. Potts, K. P. Johnson, Bennie Osburn

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


A California strain of border disease (BD) virus was isolated from the CNS and spleen of an infected lamb and was propagated without cytopathic effect on 2 tissue culture systems. Viral antigen was detected in the cytoplasm of infected cells by immunofluorescence. The growth curve of this BD virus in primary sheep choroid plexus cells revealed a latent period of 12 hours and peak viral production by 36 hours after inoculation. The growth curve in porcine kidney cells (PK-15) demonstrated a latent period of 8 hours and peak viral production by 54 hours after inoculation. The primary sheep choroid plexus cell tissue culture isolate of BD virus was then used to develop hyperimmune sera in 2 adult sheep. Neutralizing antibody was not detected until 43 days after primary inoculation when a slow antibody increase occurred in both sheep.

Original languageEnglish (US)
Pages (from-to)1460-1463
Number of pages4
JournalAmerican Journal of Veterinary Research
Issue number8
StatePublished - Aug 1 1982
Externally publishedYes

ASJC Scopus subject areas

  • veterinary(all)


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