Abstract
A California strain of border disease (BD) virus was isolated from the CNS and spleen of an infected lamb and was propagated without cytopathic effect on 2 tissue culture systems. Viral antigen was detected in the cytoplasm of infected cells by immunofluorescence. The growth curve of this BD virus in primary sheep choroid plexus cells revealed a latent period of 12 hours and peak viral production by 36 hours after inoculation. The growth curve in porcine kidney cells (PK-15) demonstrated a latent period of 8 hours and peak viral production by 54 hours after inoculation. The primary sheep choroid plexus cell tissue culture isolate of BD virus was then used to develop hyperimmune sera in 2 adult sheep. Neutralizing antibody was not detected until 43 days after primary inoculation when a slow antibody increase occurred in both sheep.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1460-1463 |
| Number of pages | 4 |
| Journal | American Journal of Veterinary Research |
| Volume | 43 |
| Issue number | 8 |
| State | Published - Aug 1 1982 |
| Externally published | Yes |
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ASJC Scopus subject areas
- veterinary(all)
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Border disease : tissue culture studies of the virus in sheep. / Potts, B. J.; Johnson, K. P.; Osburn, Bennie.
In: American Journal of Veterinary Research, Vol. 43, No. 8, 01.08.1982, p. 1460-1463.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Border disease
T2 - tissue culture studies of the virus in sheep.
AU - Potts, B. J.
AU - Johnson, K. P.
AU - Osburn, Bennie
PY - 1982/8/1
Y1 - 1982/8/1
N2 - A California strain of border disease (BD) virus was isolated from the CNS and spleen of an infected lamb and was propagated without cytopathic effect on 2 tissue culture systems. Viral antigen was detected in the cytoplasm of infected cells by immunofluorescence. The growth curve of this BD virus in primary sheep choroid plexus cells revealed a latent period of 12 hours and peak viral production by 36 hours after inoculation. The growth curve in porcine kidney cells (PK-15) demonstrated a latent period of 8 hours and peak viral production by 54 hours after inoculation. The primary sheep choroid plexus cell tissue culture isolate of BD virus was then used to develop hyperimmune sera in 2 adult sheep. Neutralizing antibody was not detected until 43 days after primary inoculation when a slow antibody increase occurred in both sheep.
AB - A California strain of border disease (BD) virus was isolated from the CNS and spleen of an infected lamb and was propagated without cytopathic effect on 2 tissue culture systems. Viral antigen was detected in the cytoplasm of infected cells by immunofluorescence. The growth curve of this BD virus in primary sheep choroid plexus cells revealed a latent period of 12 hours and peak viral production by 36 hours after inoculation. The growth curve in porcine kidney cells (PK-15) demonstrated a latent period of 8 hours and peak viral production by 54 hours after inoculation. The primary sheep choroid plexus cell tissue culture isolate of BD virus was then used to develop hyperimmune sera in 2 adult sheep. Neutralizing antibody was not detected until 43 days after primary inoculation when a slow antibody increase occurred in both sheep.
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UR - http://www.scopus.com/inward/citedby.url?scp=0020172368&partnerID=8YFLogxK
M3 - Article
C2 - 6285783
AN - SCOPUS:0020172368
VL - 43
SP - 1460
EP - 1463
JO - American Journal of Veterinary Research
JF - American Journal of Veterinary Research
SN - 0002-9645
IS - 8
ER -