Border disease: tissue culture studies of the virus in sheep.

B. J. Potts, K. P. Johnson, Bennie Osburn

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A California strain of border disease (BD) virus was isolated from the CNS and spleen of an infected lamb and was propagated without cytopathic effect on 2 tissue culture systems. Viral antigen was detected in the cytoplasm of infected cells by immunofluorescence. The growth curve of this BD virus in primary sheep choroid plexus cells revealed a latent period of 12 hours and peak viral production by 36 hours after inoculation. The growth curve in porcine kidney cells (PK-15) demonstrated a latent period of 8 hours and peak viral production by 54 hours after inoculation. The primary sheep choroid plexus cell tissue culture isolate of BD virus was then used to develop hyperimmune sera in 2 adult sheep. Neutralizing antibody was not detected until 43 days after primary inoculation when a slow antibody increase occurred in both sheep.

Original languageEnglish (US)
Pages (from-to)1460-1463
Number of pages4
JournalAmerican Journal of Veterinary Research
Volume43
Issue number8
StatePublished - Aug 1 1982
Externally publishedYes

Fingerprint

Border Disease
border disease
Border disease virus
tissue culture
Sheep
Viruses
choroid plexus
sheep
viruses
latent period
Choroid Plexus
cytopathogenicity
Viral Antigens
viral antigens
cells
kidney cells
Growth
Neutralizing Antibodies
neutralizing antibodies
fluorescent antibody technique

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Potts, B. J., Johnson, K. P., & Osburn, B. (1982). Border disease: tissue culture studies of the virus in sheep. American Journal of Veterinary Research, 43(8), 1460-1463.

Border disease : tissue culture studies of the virus in sheep. / Potts, B. J.; Johnson, K. P.; Osburn, Bennie.

In: American Journal of Veterinary Research, Vol. 43, No. 8, 01.08.1982, p. 1460-1463.

Research output: Contribution to journalArticle

Potts, BJ, Johnson, KP & Osburn, B 1982, 'Border disease: tissue culture studies of the virus in sheep.', American Journal of Veterinary Research, vol. 43, no. 8, pp. 1460-1463.
Potts, B. J. ; Johnson, K. P. ; Osburn, Bennie. / Border disease : tissue culture studies of the virus in sheep. In: American Journal of Veterinary Research. 1982 ; Vol. 43, No. 8. pp. 1460-1463.
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