Boosting of SIV-specific T cell responses in rhesus macaques that resist repeated intravaginal challenge with SIVmac251

Barbara Shacklett, Preston A. Marx, Binhua Ling, Ronald S. Veazey, Amara Luckay, Walter J. Moretto, David T. Wilkens, Hu Jennifer, Zimra R. Israel, Douglas F. Nixon

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Despite repeated high-risk exposure to infectious HIV-1, some individuals remain HIV-1 seronegative and apparently uninfected. The use of nonhuman primate model systems to study SIVmac transmission may help to elucidate the factors responsible for protection in exposed, seronegative (ESN) humans. In an earlier vaccination study, three control rhesus macaques that were exposed to three sequential intravaginal challenges with pathogenic SIVmac251 failed to show evidence of infection after 5 years of observation. 51Cr release assay results suggested that these animals had low-level cytotoxic T lymphocyte responses to SIVmac proteins. We hypothesized that these responses might be an important component of protection from mucosal challenge. We performed an additional intravaginal challenge of all three macaques and monitored SIV-specific T cell responses in peripheral blood, using the sensitive enzyme-linked immunospot (ELISpot) assay. After the fourth challenge, one animal became infected; this animal did not mount a strong SIV-specific T cell response. Two other macaques remained uninfected as determined by peripheral blood mononuclear cell (PBMC) coculture, polymerase chain reaction (PCR), and branched DNA (bDNA) analysis of peripheral blood and lymphoid tissues, but demonstrated boosting of SIV-specific T cell responses after challenge. These results support a protective role for SIVmac-specific T cells in repeatedly exposed, persistently seronegative rhesus macaques.

Original languageEnglish (US)
Pages (from-to)1081-1088
Number of pages8
JournalAIDS Research and Human Retroviruses
Volume18
Issue number14
DOIs
StatePublished - 2002

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Macaca mulatta
T-Lymphocytes
Macaca
HIV-1
Enzyme-Linked Immunospot Assay
Cytotoxic T-Lymphocytes
Lymphoid Tissue
Coculture Techniques
Primates
Blood Cells
Vaccination
Observation
Polymerase Chain Reaction
DNA
Infection
Proteins

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Boosting of SIV-specific T cell responses in rhesus macaques that resist repeated intravaginal challenge with SIVmac251. / Shacklett, Barbara; Marx, Preston A.; Ling, Binhua; Veazey, Ronald S.; Luckay, Amara; Moretto, Walter J.; Wilkens, David T.; Jennifer, Hu; Israel, Zimra R.; Nixon, Douglas F.

In: AIDS Research and Human Retroviruses, Vol. 18, No. 14, 2002, p. 1081-1088.

Research output: Contribution to journalArticle

Shacklett, B, Marx, PA, Ling, B, Veazey, RS, Luckay, A, Moretto, WJ, Wilkens, DT, Jennifer, H, Israel, ZR & Nixon, DF 2002, 'Boosting of SIV-specific T cell responses in rhesus macaques that resist repeated intravaginal challenge with SIVmac251', AIDS Research and Human Retroviruses, vol. 18, no. 14, pp. 1081-1088. https://doi.org/10.1089/08892220260235434
Shacklett, Barbara ; Marx, Preston A. ; Ling, Binhua ; Veazey, Ronald S. ; Luckay, Amara ; Moretto, Walter J. ; Wilkens, David T. ; Jennifer, Hu ; Israel, Zimra R. ; Nixon, Douglas F. / Boosting of SIV-specific T cell responses in rhesus macaques that resist repeated intravaginal challenge with SIVmac251. In: AIDS Research and Human Retroviruses. 2002 ; Vol. 18, No. 14. pp. 1081-1088.
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