Bone morphogenetic protein-5 (BMP-5) promotes dendritic growth in cultured sympathetic neurons

Hiroko N. Beck, Karen Drahushuk, David B. Jacoby, Dennis Higgins, Pamela J Lein

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Background: BMP-5 is expressed in the nervous system throughout development and into adulthood. However its effects on neural tissues are not well defined. BMP-5 is a member of the 60A subgroup of BMPs, other members of which have been shown to stimulate dendritic growth in central and peripheral neurons. We therefore examined the possibility that BMP-5 similarly enhances dendritic growth in cultured sympathetic neurons. Results: Sympathetic neurons cultured in the absence of serum or glial cells do not form dendrites; however, addition of BMP-5 causes these neurons to extend multiple dendritic processes, which is preceded by an increase in phosphorylation of the Smad-1 transcription factor. The dendrite-promoting activity of BMP-5 is significantly inhibited by the BMP antagonists noggin and follistatin and by a BMPR-IA-Fc chimeric protein. RT-PCR and immunocytochemical analyses indicate that BMP-5 mRNA and protein are expressed in the superior cervical ganglia (SCG) during times of initial growth and rapid expansion of the dendritic arbor. Conclusions: These data suggest a role for BMP-5 in regulating dendritic growth in sympathetic neurons. The signaling pathway that mediates the dendrite-promoting activity of BMP-5 may involve binding to BMPR-IA and activation of Smad-1, and relative levels of BMP antagonists such as noggin and follistatin may modulate BMP-5 signaling. Since BMP-5 is expressed at relatively high levels not only in the developing but also the adult nervous system, these findings suggest the possibility that BMP-5 regulates dendritic morphology not only in the developing, but also the adult nervous system.

Original languageEnglish (US)
Article number12
JournalBMC Neuroscience
Volume2
DOIs
StatePublished - Sep 11 2001
Externally publishedYes

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Bone Morphogenetic Protein 5
Neurons
Growth
Dendrites
Follistatin
Nervous System
Superior Cervical Ganglion
Neuroglia

ASJC Scopus subject areas

  • Medicine(all)
  • Clinical Neurology
  • Neuroscience(all)

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Bone morphogenetic protein-5 (BMP-5) promotes dendritic growth in cultured sympathetic neurons. / Beck, Hiroko N.; Drahushuk, Karen; Jacoby, David B.; Higgins, Dennis; Lein, Pamela J.

In: BMC Neuroscience, Vol. 2, 12, 11.09.2001.

Research output: Contribution to journalArticle

Beck, Hiroko N. ; Drahushuk, Karen ; Jacoby, David B. ; Higgins, Dennis ; Lein, Pamela J. / Bone morphogenetic protein-5 (BMP-5) promotes dendritic growth in cultured sympathetic neurons. In: BMC Neuroscience. 2001 ; Vol. 2.
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