Bombesin stimulates the in vitro growth of a human gastric cancer cell line

Richard J Bold, Patrick S. Lowry, Jin Ishizuka, James F. Battey, Courtney M. Townsend, James C. Thompson

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Bombesin (BBS) and its mammalian equivalent, gastrin-releasing peptide (GRP), exhibit diverse biological functions, including that of a neurotransmitter, a regulator of gastrointestinal hormone release, and a trophic factor for various normal and neoplastic tissues. Bombesin stimulates the growth of normal cells of the stomach, pancreas, and bronchial epithelium as well as cells in breast cancer, gastrinoma, and small cell lung cancer. The purpose of this study was to determine whether BBS regulates the growth of a human gastric cancer cell line (SIIA) in vitro, and if so, to examine the mechanisms of signal-transduction that are involved. We found that BBS stimulated the growth of SI[A cells in vitro. The GRP receptor antagonists, BIM 26189 and BIM 26226, had no effect on growth of SIIA cells. Although these antagonists blocked the BBS-induced increase of [Ca2+](i), they failed to block the growth-stimulatory effect of BBS. BBS stimulated intracellular tyrosine phosphorylation of multipe proteins, with a predominant protein of apparent molecular weight of 125 kDa. Inhibition of intracellular tyrosine kinases by tyrphostin blocked the growth-stimulatory effect of BBS on SI[A cells. These results indicate that BBS exerts its trophic effect on SIIA cells through a receptor(s) linked to tyrosine kinase pathway, but not to the phospholipase C (PLC) pathway.

Original languageEnglish (US)
Pages (from-to)519-525
Number of pages7
JournalJournal of Cellular Physiology
Volume161
Issue number3
DOIs
StatePublished - Dec 1994

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

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    Bold, R. J., Lowry, P. S., Ishizuka, J., Battey, J. F., Townsend, C. M., & Thompson, J. C. (1994). Bombesin stimulates the in vitro growth of a human gastric cancer cell line. Journal of Cellular Physiology, 161(3), 519-525. https://doi.org/10.1002/jcp.1041610315