BMI1 regulation of self-renewal and multipotency in human mesenchymal stem cells

Yunjoon Jung, Jan Nolta

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

We have previously described generation of mesenchymal stem cells (MSCs) from human embryonic and induced pluripotent stem cells. One of the central questions in stem cell biology is to understand how stem cells regulate the decision to self-renew vs. differentiate, at the molecular level. In the current studies we used loss-of-function and gain-of-function analyses in primary human MSCs to demonstrate that BMI1 is a critical regulator for self-renewal and multipotency in this interesting cell type. Knockdown of BMI1 in MSCs reduced self-renewal by upregulation of p16INK4A and increased apoptosis. Knockdown of p16INK4A partially rescued the self-renewal defect in MSCs with loss of BMI1. Overexpressed BMI1 reduced apoptosis and increased cell proliferation by repressing p16INK4A. Loss of BMI1 resulted in deregulation of PPARγ, an adipogenic factor, and imprinted gene network (IGN), which blocks osteogenesis. Knockdown of PPARγ or IGN in BMI1 defect models restored osteogenesis. Overexpression of BMI1 repressed transcripts of RUNX2 and PPARγ, in osteogenesis and adipogenesis, respectively, which lead to decreased lineage specification potential in MSCs. These data show that BMI1 regulates cell proliferation, apoptosis, and differentiation of human MSCs.

Original languageEnglish (US)
Pages (from-to)131-140
Number of pages10
JournalCurrent Stem Cell Research and Therapy
Volume11
Issue number2
StatePublished - Jan 1 2016

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Keywords

  • Adipogenesis
  • Cell cycle
  • Epigenetics
  • IPSCs
  • Mesenchymal stem cells
  • Osteogenesis
  • Polycomb-repressive complex 1
  • Proliferation
  • Stem cell differentiation

ASJC Scopus subject areas

  • Medicine (miscellaneous)

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