Bluetongue virus genome remains stable throughout prolonged infection of cattle.

H. W. Heidner, Nigel J Maclachlan, F. J. Fuller, R. G. Richards, L. E. Whetter

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Infection of three calves with a highly plaque-purified strain of bluetongue virus (BTV) resulted in prolonged infections, during which virus and neutralizing antibodies co-circulated in peripheral blood. Oligonucleotide fingerprint analyses of the original challenge virus and of the final virus isolate obtained from each calf demonstrated the BTV genome to remain stable throughout prolonged infection as no differences in fingerprint patterns were detected. Six neutralizing monoclonal antibodies (MAbs), and a polyclonal rabbit antiserum, were produced against the challenge virus. This panel of MAbs recognized at least two distinct neutralizing epitopes as demonstrated by immune precipitation. Neutralizing epitopes remained stable through the prolonged infections, as all MAbs and the polyclonal rabbit antiserum neutralized the challenge virus and the final calf isolates to equivalent titres. These results suggest that antigenic drift is not the mechanism by which BTV is able to persist in cattle in spite of a strong humoral immune response.

Original languageEnglish (US)
JournalJournal of General Virology
Volume69
StatePublished - Oct 1988

Fingerprint

Bluetongue virus
Genome
Viruses
Infection
Monoclonal Antibodies
Dermatoglyphics
Neutralizing Antibodies
Immune Sera
Epitopes
Rabbits
Humoral Immunity
Immunoprecipitation
Oligonucleotides

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Bluetongue virus genome remains stable throughout prolonged infection of cattle. / Heidner, H. W.; Maclachlan, Nigel J; Fuller, F. J.; Richards, R. G.; Whetter, L. E.

In: Journal of General Virology, Vol. 69, 10.1988.

Research output: Contribution to journalArticle

Heidner, H. W. ; Maclachlan, Nigel J ; Fuller, F. J. ; Richards, R. G. ; Whetter, L. E. / Bluetongue virus genome remains stable throughout prolonged infection of cattle. In: Journal of General Virology. 1988 ; Vol. 69.
@article{5077069d737244a7a00b2881b4954b56,
title = "Bluetongue virus genome remains stable throughout prolonged infection of cattle.",
abstract = "Infection of three calves with a highly plaque-purified strain of bluetongue virus (BTV) resulted in prolonged infections, during which virus and neutralizing antibodies co-circulated in peripheral blood. Oligonucleotide fingerprint analyses of the original challenge virus and of the final virus isolate obtained from each calf demonstrated the BTV genome to remain stable throughout prolonged infection as no differences in fingerprint patterns were detected. Six neutralizing monoclonal antibodies (MAbs), and a polyclonal rabbit antiserum, were produced against the challenge virus. This panel of MAbs recognized at least two distinct neutralizing epitopes as demonstrated by immune precipitation. Neutralizing epitopes remained stable through the prolonged infections, as all MAbs and the polyclonal rabbit antiserum neutralized the challenge virus and the final calf isolates to equivalent titres. These results suggest that antigenic drift is not the mechanism by which BTV is able to persist in cattle in spite of a strong humoral immune response.",
author = "Heidner, {H. W.} and Maclachlan, {Nigel J} and Fuller, {F. J.} and Richards, {R. G.} and Whetter, {L. E.}",
year = "1988",
month = "10",
language = "English (US)",
volume = "69",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",

}

TY - JOUR

T1 - Bluetongue virus genome remains stable throughout prolonged infection of cattle.

AU - Heidner, H. W.

AU - Maclachlan, Nigel J

AU - Fuller, F. J.

AU - Richards, R. G.

AU - Whetter, L. E.

PY - 1988/10

Y1 - 1988/10

N2 - Infection of three calves with a highly plaque-purified strain of bluetongue virus (BTV) resulted in prolonged infections, during which virus and neutralizing antibodies co-circulated in peripheral blood. Oligonucleotide fingerprint analyses of the original challenge virus and of the final virus isolate obtained from each calf demonstrated the BTV genome to remain stable throughout prolonged infection as no differences in fingerprint patterns were detected. Six neutralizing monoclonal antibodies (MAbs), and a polyclonal rabbit antiserum, were produced against the challenge virus. This panel of MAbs recognized at least two distinct neutralizing epitopes as demonstrated by immune precipitation. Neutralizing epitopes remained stable through the prolonged infections, as all MAbs and the polyclonal rabbit antiserum neutralized the challenge virus and the final calf isolates to equivalent titres. These results suggest that antigenic drift is not the mechanism by which BTV is able to persist in cattle in spite of a strong humoral immune response.

AB - Infection of three calves with a highly plaque-purified strain of bluetongue virus (BTV) resulted in prolonged infections, during which virus and neutralizing antibodies co-circulated in peripheral blood. Oligonucleotide fingerprint analyses of the original challenge virus and of the final virus isolate obtained from each calf demonstrated the BTV genome to remain stable throughout prolonged infection as no differences in fingerprint patterns were detected. Six neutralizing monoclonal antibodies (MAbs), and a polyclonal rabbit antiserum, were produced against the challenge virus. This panel of MAbs recognized at least two distinct neutralizing epitopes as demonstrated by immune precipitation. Neutralizing epitopes remained stable through the prolonged infections, as all MAbs and the polyclonal rabbit antiserum neutralized the challenge virus and the final calf isolates to equivalent titres. These results suggest that antigenic drift is not the mechanism by which BTV is able to persist in cattle in spite of a strong humoral immune response.

UR - http://www.scopus.com/inward/record.url?scp=0024094485&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024094485&partnerID=8YFLogxK

M3 - Article

C2 - 2459303

AN - SCOPUS:0024094485

VL - 69

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

ER -