Abstract
The cDNA coding for the major nonstructural protein, NS1, of bluetongue serotype 17 (BTV-17) was cloned previously. Using pUC plasmids, we have successfully expressed the NS1 protein in Escherichia coli as a lacZ-NS1 fusion protein. The recombinant NS1 protein reacted with rabbit anti-BTV-17 antiserum, and was thus immunologically indistinguishable from the native BTV-17 NS1 protein. This was the first bluetongue viral protein to be produced in a bacterial system.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 892-899 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 162 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jul 31 1989 |
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ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Bluetongue virus-17 fusion protein NS1 expressed in Escherichia coli by pUC vectors. / Wang, Lin fa; Doi, Roy H.; Chuang, Linda F.; Osburn, Bennie; Maisonnave, Jaqueline; Benjamini, Eliezer; Chuang, Ronald Y.
In: Biochemical and Biophysical Research Communications, Vol. 162, No. 2, 31.07.1989, p. 892-899.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bluetongue virus-17 fusion protein NS1 expressed in Escherichia coli by pUC vectors
AU - Wang, Lin fa
AU - Doi, Roy H.
AU - Chuang, Linda F.
AU - Osburn, Bennie
AU - Maisonnave, Jaqueline
AU - Benjamini, Eliezer
AU - Chuang, Ronald Y.
PY - 1989/7/31
Y1 - 1989/7/31
N2 - The cDNA coding for the major nonstructural protein, NS1, of bluetongue serotype 17 (BTV-17) was cloned previously. Using pUC plasmids, we have successfully expressed the NS1 protein in Escherichia coli as a lacZ-NS1 fusion protein. The recombinant NS1 protein reacted with rabbit anti-BTV-17 antiserum, and was thus immunologically indistinguishable from the native BTV-17 NS1 protein. This was the first bluetongue viral protein to be produced in a bacterial system.
AB - The cDNA coding for the major nonstructural protein, NS1, of bluetongue serotype 17 (BTV-17) was cloned previously. Using pUC plasmids, we have successfully expressed the NS1 protein in Escherichia coli as a lacZ-NS1 fusion protein. The recombinant NS1 protein reacted with rabbit anti-BTV-17 antiserum, and was thus immunologically indistinguishable from the native BTV-17 NS1 protein. This was the first bluetongue viral protein to be produced in a bacterial system.
UR - http://www.scopus.com/inward/record.url?scp=0024380576&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024380576&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(89)92393-0
DO - 10.1016/0006-291X(89)92393-0
M3 - Article
C2 - 2547378
AN - SCOPUS:0024380576
VL - 162
SP - 892
EP - 899
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -