Abstract
The cDNA coding for the major nonstructural protein, NS1, of bluetongue serotype 17 (BTV-17) was cloned previously. Using pUC plasmids, we have successfully expressed the NS1 protein in Escherichia coli as a lacZ-NS1 fusion protein. The recombinant NS1 protein reacted with rabbit anti-BTV-17 antiserum, and was thus immunologically indistinguishable from the native BTV-17 NS1 protein. This was the first bluetongue viral protein to be produced in a bacterial system.
Original language | English (US) |
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Pages (from-to) | 892-899 |
Number of pages | 8 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 162 |
Issue number | 2 |
DOIs | |
State | Published - Jul 31 1989 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology