Bluetongue virus-17 fusion protein NS1 expressed in Escherichia coli by pUC vectors

Lin fa Wang; Roy H. Doi; Linda F. Chuang; Bennie Osburn; Jaqueline Maisonnave; Eliezer Benjamini; Ronald Y. Chuang

doi:10.1016/0006-291X(89)92393-0

Bluetongue virus-17 fusion protein NS1 expressed in Escherichia coli by pUC vectors

Lin fa Wang, Roy H. Doi, Linda F. Chuang, Bennie Osburn, Jaqueline Maisonnave, Eliezer Benjamini, Ronald Y. Chuang

UC Davis School of Medicine

Research output: Contribution to journal › Article

4 Scopus citations

Abstract

The cDNA coding for the major nonstructural protein, NS1, of bluetongue serotype 17 (BTV-17) was cloned previously. Using pUC plasmids, we have successfully expressed the NS1 protein in Escherichia coli as a lacZ-NS1 fusion protein. The recombinant NS1 protein reacted with rabbit anti-BTV-17 antiserum, and was thus immunologically indistinguishable from the native BTV-17 NS1 protein. This was the first bluetongue viral protein to be produced in a bacterial system.

Original language	English (US)
Pages (from-to)	892-899
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	162
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(89)92393-0
State	Published - Jul 31 1989

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Wang, L. F., Doi, R. H., Chuang, L. F., Osburn, B., Maisonnave, J., Benjamini, E., & Chuang, R. Y. (1989). Bluetongue virus-17 fusion protein NS1 expressed in Escherichia coli by pUC vectors. Biochemical and Biophysical Research Communications, 162(2), 892-899. https://doi.org/10.1016/0006-291X(89)92393-0

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abstract = "The cDNA coding for the major nonstructural protein, NS1, of bluetongue serotype 17 (BTV-17) was cloned previously. Using pUC plasmids, we have successfully expressed the NS1 protein in Escherichia coli as a lacZ-NS1 fusion protein. The recombinant NS1 protein reacted with rabbit anti-BTV-17 antiserum, and was thus immunologically indistinguishable from the native BTV-17 NS1 protein. This was the first bluetongue viral protein to be produced in a bacterial system.",

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AU - Benjamini, Eliezer

AU - Chuang, Ronald Y.

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