Bluetongue virus-17 fusion protein NS1 expressed in Escherichia coli by pUC vectors

Lin fa Wang, Roy H. Doi, Linda F. Chuang, Bennie Osburn, Jaqueline Maisonnave, Eliezer Benjamini, Ronald Y. Chuang

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The cDNA coding for the major nonstructural protein, NS1, of bluetongue serotype 17 (BTV-17) was cloned previously. Using pUC plasmids, we have successfully expressed the NS1 protein in Escherichia coli as a lacZ-NS1 fusion protein. The recombinant NS1 protein reacted with rabbit anti-BTV-17 antiserum, and was thus immunologically indistinguishable from the native BTV-17 NS1 protein. This was the first bluetongue viral protein to be produced in a bacterial system.

Original languageEnglish (US)
Pages (from-to)892-899
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume162
Issue number2
DOIs
StatePublished - Jul 31 1989

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Wang, L. F., Doi, R. H., Chuang, L. F., Osburn, B., Maisonnave, J., Benjamini, E., & Chuang, R. Y. (1989). Bluetongue virus-17 fusion protein NS1 expressed in Escherichia coli by pUC vectors. Biochemical and Biophysical Research Communications, 162(2), 892-899. https://doi.org/10.1016/0006-291X(89)92393-0