Bluetongue virus-17 fusion protein NS1 expressed in Escherichia coli by pUC vectors

Lin fa Wang, Roy H. Doi, Linda F. Chuang, Bennie Osburn, Jaqueline Maisonnave, Eliezer Benjamini, Ronald Y. Chuang

Research output: Contribution to journal › Article

4 Citations (Scopus)

Abstract

The cDNA coding for the major nonstructural protein, NS1, of bluetongue serotype 17 (BTV-17) was cloned previously. Using pUC plasmids, we have successfully expressed the NS1 protein in Escherichia coli as a lacZ-NS1 fusion protein. The recombinant NS1 protein reacted with rabbit anti-BTV-17 antiserum, and was thus immunologically indistinguishable from the native BTV-17 NS1 protein. This was the first bluetongue viral protein to be produced in a bacterial system.

Original languageEnglish (US)
Pages (from-to)892-899
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume162
Issue number2
DOIs
StatePublished - Jul 31 1989

Fingerprint

Bluetongue
Viral Fusion Proteins
Bluetongue virus
Viruses
Escherichia coli
Fusion reactions
Escherichia coli Proteins
Viral Proteins
Recombinant Proteins
Immune Sera
Proteins
Plasmids
Complementary DNA
Rabbits
Recombinant proteins

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Wang, L. F., Doi, R. H., Chuang, L. F., Osburn, B., Maisonnave, J., Benjamini, E., & Chuang, R. Y. (1989). Bluetongue virus-17 fusion protein NS1 expressed in Escherichia coli by pUC vectors. Biochemical and Biophysical Research Communications, 162(2), 892-899. https://doi.org/10.1016/0006-291X(89)92393-0

Bluetongue virus-17 fusion protein NS1 expressed in Escherichia coli by pUC vectors. / Wang, Lin fa; Doi, Roy H.; Chuang, Linda F.; Osburn, Bennie; Maisonnave, Jaqueline; Benjamini, Eliezer; Chuang, Ronald Y.

In: Biochemical and Biophysical Research Communications, Vol. 162, No. 2, 31.07.1989, p. 892-899.

Research output: Contribution to journal › Article

Wang, LF, Doi, RH, Chuang, LF, Osburn, B, Maisonnave, J, Benjamini, E & Chuang, RY 1989, 'Bluetongue virus-17 fusion protein NS1 expressed in Escherichia coli by pUC vectors', Biochemical and Biophysical Research Communications, vol. 162, no. 2, pp. 892-899. https://doi.org/10.1016/0006-291X(89)92393-0
Wang LF, Doi RH, Chuang LF, Osburn B, Maisonnave J, Benjamini E et al. Bluetongue virus-17 fusion protein NS1 expressed in Escherichia coli by pUC vectors. Biochemical and Biophysical Research Communications. 1989 Jul 31;162(2):892-899. https://doi.org/10.1016/0006-291X(89)92393-0
Wang, Lin fa ; Doi, Roy H. ; Chuang, Linda F. ; Osburn, Bennie ; Maisonnave, Jaqueline ; Benjamini, Eliezer ; Chuang, Ronald Y. / Bluetongue virus-17 fusion protein NS1 expressed in Escherichia coli by pUC vectors. In: Biochemical and Biophysical Research Communications. 1989 ; Vol. 162, No. 2. pp. 892-899.
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