Blood transfusion is associated with donor leukocyte microchimerism in trauma patients

Garth H Utter, John T Owings, Tzong Hae Lee, Teresa G. Paglieroni, William F. Reed, Robert C. Gosselin, Paul V. Holland, Michael P. Busch, Lena M. Napolitano

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Introduction: Blood transfusion can result in survival of donor leukocyte subpopulations in the recipient. Persistence of donor leukocytes in the transfusion recipient is termed microchimerism. Microchimerism likely reflects engraftment of the recipient with donor hematopoietic stem cells and is very uncommon with transfusion for elective surgery, sickle cell anemia, thalassemia, and HIV. We have found, however, that microchimerism may be more common in trauma patients. Objective: To determine how frequently transfusion after trauma is associated with microchimerism. Methods: We prospectively enrolled 45 trauma patients who were transfused ≥2 units of PRBCs. We sampled blood before hospital discharge and determined microchimerism by polymerase chain reaction (PCR) analysis of specimens using quantitative allele-specific HLA DR assays to detect non-recipient alleles. Data are expressed as median with interquartile range. Results: Patients had a median age of 38 (interquartile range 25, 58) years, ISS of 19 (13, 29), and mortality of 7%. Seventy-eight percent were men, and 84% had blunt trauma. Patients received a median of 6 (4, 16) (range 2, 87) units of PRBCs. Of the 45 patients, 24 (53%) had evidence of microchimerism. Compared with patients without evidence of microchimerism, these patients had no difference in mean age, gender, ISS, units of PRBCs transfused, time from transfusion to blood sampling, or proportion that underwent splenectomy. Twenty-one of the 24 patients with microchimerism had only 1 or 2 non-recipient DR alleles identified by PCR. Conclusions: Transfusion after trauma is associated with over half of recipients having evidence of microchimerism. Age, sex, ISS, and splenectomy of the recipient and the number of transfused units did not correlate with microchimerism. Because the median time from transfusion to sampling for PCR analysis was not longer in the group without microchimerism, it is unlikely microchimerism is due merely to failure of the recipient to clear transfused donor leukocytes.

Original languageEnglish (US)
Pages (from-to)702-708
Number of pages7
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume57
Issue number4
DOIs
StatePublished - Oct 2004

Fingerprint

Chimerism
Blood Transfusion
Leukocytes
Tissue Donors
Wounds and Injuries
Alleles
Splenectomy
Polymerase Chain Reaction
Leukocyte Transfusion
Thalassemia
Sickle Cell Anemia
HLA-DR Antigens
Hematopoietic Stem Cells

Keywords

  • Alloimmunization
  • Blood transfusion
  • Leukocytes
  • Microchimerism
  • Trauma

ASJC Scopus subject areas

  • Surgery

Cite this

Blood transfusion is associated with donor leukocyte microchimerism in trauma patients. / Utter, Garth H; Owings, John T; Lee, Tzong Hae; Paglieroni, Teresa G.; Reed, William F.; Gosselin, Robert C.; Holland, Paul V.; Busch, Michael P.; Napolitano, Lena M.

In: Journal of Trauma - Injury, Infection and Critical Care, Vol. 57, No. 4, 10.2004, p. 702-708.

Research output: Contribution to journalArticle

Utter, Garth H ; Owings, John T ; Lee, Tzong Hae ; Paglieroni, Teresa G. ; Reed, William F. ; Gosselin, Robert C. ; Holland, Paul V. ; Busch, Michael P. ; Napolitano, Lena M. / Blood transfusion is associated with donor leukocyte microchimerism in trauma patients. In: Journal of Trauma - Injury, Infection and Critical Care. 2004 ; Vol. 57, No. 4. pp. 702-708.
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abstract = "Introduction: Blood transfusion can result in survival of donor leukocyte subpopulations in the recipient. Persistence of donor leukocytes in the transfusion recipient is termed microchimerism. Microchimerism likely reflects engraftment of the recipient with donor hematopoietic stem cells and is very uncommon with transfusion for elective surgery, sickle cell anemia, thalassemia, and HIV. We have found, however, that microchimerism may be more common in trauma patients. Objective: To determine how frequently transfusion after trauma is associated with microchimerism. Methods: We prospectively enrolled 45 trauma patients who were transfused ≥2 units of PRBCs. We sampled blood before hospital discharge and determined microchimerism by polymerase chain reaction (PCR) analysis of specimens using quantitative allele-specific HLA DR assays to detect non-recipient alleles. Data are expressed as median with interquartile range. Results: Patients had a median age of 38 (interquartile range 25, 58) years, ISS of 19 (13, 29), and mortality of 7{\%}. Seventy-eight percent were men, and 84{\%} had blunt trauma. Patients received a median of 6 (4, 16) (range 2, 87) units of PRBCs. Of the 45 patients, 24 (53{\%}) had evidence of microchimerism. Compared with patients without evidence of microchimerism, these patients had no difference in mean age, gender, ISS, units of PRBCs transfused, time from transfusion to blood sampling, or proportion that underwent splenectomy. Twenty-one of the 24 patients with microchimerism had only 1 or 2 non-recipient DR alleles identified by PCR. Conclusions: Transfusion after trauma is associated with over half of recipients having evidence of microchimerism. Age, sex, ISS, and splenectomy of the recipient and the number of transfused units did not correlate with microchimerism. Because the median time from transfusion to sampling for PCR analysis was not longer in the group without microchimerism, it is unlikely microchimerism is due merely to failure of the recipient to clear transfused donor leukocytes.",
keywords = "Alloimmunization, Blood transfusion, Leukocytes, Microchimerism, Trauma",
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T1 - Blood transfusion is associated with donor leukocyte microchimerism in trauma patients

AU - Utter, Garth H

AU - Owings, John T

AU - Lee, Tzong Hae

AU - Paglieroni, Teresa G.

AU - Reed, William F.

AU - Gosselin, Robert C.

AU - Holland, Paul V.

AU - Busch, Michael P.

AU - Napolitano, Lena M.

PY - 2004/10

Y1 - 2004/10

N2 - Introduction: Blood transfusion can result in survival of donor leukocyte subpopulations in the recipient. Persistence of donor leukocytes in the transfusion recipient is termed microchimerism. Microchimerism likely reflects engraftment of the recipient with donor hematopoietic stem cells and is very uncommon with transfusion for elective surgery, sickle cell anemia, thalassemia, and HIV. We have found, however, that microchimerism may be more common in trauma patients. Objective: To determine how frequently transfusion after trauma is associated with microchimerism. Methods: We prospectively enrolled 45 trauma patients who were transfused ≥2 units of PRBCs. We sampled blood before hospital discharge and determined microchimerism by polymerase chain reaction (PCR) analysis of specimens using quantitative allele-specific HLA DR assays to detect non-recipient alleles. Data are expressed as median with interquartile range. Results: Patients had a median age of 38 (interquartile range 25, 58) years, ISS of 19 (13, 29), and mortality of 7%. Seventy-eight percent were men, and 84% had blunt trauma. Patients received a median of 6 (4, 16) (range 2, 87) units of PRBCs. Of the 45 patients, 24 (53%) had evidence of microchimerism. Compared with patients without evidence of microchimerism, these patients had no difference in mean age, gender, ISS, units of PRBCs transfused, time from transfusion to blood sampling, or proportion that underwent splenectomy. Twenty-one of the 24 patients with microchimerism had only 1 or 2 non-recipient DR alleles identified by PCR. Conclusions: Transfusion after trauma is associated with over half of recipients having evidence of microchimerism. Age, sex, ISS, and splenectomy of the recipient and the number of transfused units did not correlate with microchimerism. Because the median time from transfusion to sampling for PCR analysis was not longer in the group without microchimerism, it is unlikely microchimerism is due merely to failure of the recipient to clear transfused donor leukocytes.

AB - Introduction: Blood transfusion can result in survival of donor leukocyte subpopulations in the recipient. Persistence of donor leukocytes in the transfusion recipient is termed microchimerism. Microchimerism likely reflects engraftment of the recipient with donor hematopoietic stem cells and is very uncommon with transfusion for elective surgery, sickle cell anemia, thalassemia, and HIV. We have found, however, that microchimerism may be more common in trauma patients. Objective: To determine how frequently transfusion after trauma is associated with microchimerism. Methods: We prospectively enrolled 45 trauma patients who were transfused ≥2 units of PRBCs. We sampled blood before hospital discharge and determined microchimerism by polymerase chain reaction (PCR) analysis of specimens using quantitative allele-specific HLA DR assays to detect non-recipient alleles. Data are expressed as median with interquartile range. Results: Patients had a median age of 38 (interquartile range 25, 58) years, ISS of 19 (13, 29), and mortality of 7%. Seventy-eight percent were men, and 84% had blunt trauma. Patients received a median of 6 (4, 16) (range 2, 87) units of PRBCs. Of the 45 patients, 24 (53%) had evidence of microchimerism. Compared with patients without evidence of microchimerism, these patients had no difference in mean age, gender, ISS, units of PRBCs transfused, time from transfusion to blood sampling, or proportion that underwent splenectomy. Twenty-one of the 24 patients with microchimerism had only 1 or 2 non-recipient DR alleles identified by PCR. Conclusions: Transfusion after trauma is associated with over half of recipients having evidence of microchimerism. Age, sex, ISS, and splenectomy of the recipient and the number of transfused units did not correlate with microchimerism. Because the median time from transfusion to sampling for PCR analysis was not longer in the group without microchimerism, it is unlikely microchimerism is due merely to failure of the recipient to clear transfused donor leukocytes.

KW - Alloimmunization

KW - Blood transfusion

KW - Leukocytes

KW - Microchimerism

KW - Trauma

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