Blood flow and metabolic regulation in seal muscle during apnea

Paul J. Ponganis, Ulrike Kreutzer, Torre K. Stockard, Ping Chang Lin, Napapon Sailasuta, Tuan Khan Tran, Ralph Hurd, Thomas Jue

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

In order to examine myoglobin (Mb) function and metabolic responses of seal muscle during progressive ischemia and hypoxemia, Mb saturation and high-energy phosphate levels were monitored with NMR spectroscopy during sleep apnea in elephant seals (Mirounga angustirostris). Muscle blood flow (MBF) was measured with laser-Doppler flowmetry (LDF). During six, spontaneous, 8-12 min apneas of an unrestrained juvenile seal, apneic MBF decreased to 46±10% of the mean eupneic MBF. By the end of apnea, MBF reached 31±8% of the eupneic value. The t1/2 for 90% decline in apneic MBF was 1.9±1.2 min. The initial post-apneic peak in MBF occurred within 0.20±0.04 min after the start of eupnea. NMR measurements revealed that Mb desaturated rapidly from its eupenic resting level to a lower steady state value within 4 min after the onset of apnea at rates between 1.7±1.0 and 3.8±1.5% min -1, which corresponded to a muscle O2 depletion rate of 1-2.3 ml O2 kg-1 min-1. High-energy phosphate levels did not change with apnea. During the transition from apnea to eupnea, Mb resaturated to 95% of its resting level within the first minute. Despite the high Mb concentration in seal muscle, experiments detected Mb diffusing with a translational diffusion coefficient of 4.5×10-7cm 2s-1, consistent with the value observed in rat myocardium. Equipoise PO2 analysis revealed that Mb is the predominant intracellular O2 transporter in elephant seals during eupnea and apnea.

Original languageEnglish (US)
Pages (from-to)3323-3332
Number of pages10
JournalJournal of Experimental Biology
Volume211
Issue number20
DOIs
StatePublished - Oct 15 2008

Fingerprint

apnea
Apnea
seals
blood flow
myoglobin
Myoglobin
muscle
blood
Muscles
muscles
Earless Seals
Mirounga
elephant
nuclear magnetic resonance
Phosphates
phosphate
phosphates
sleep apnea
Mirounga angustirostris
regulation

Keywords

  • Doppler
  • Hemodynamics
  • Muscle
  • Myoglobin
  • NMR
  • Oxygen

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Physiology
  • Insect Science
  • Aquatic Science

Cite this

Ponganis, P. J., Kreutzer, U., Stockard, T. K., Lin, P. C., Sailasuta, N., Tran, T. K., ... Jue, T. (2008). Blood flow and metabolic regulation in seal muscle during apnea. Journal of Experimental Biology, 211(20), 3323-3332. https://doi.org/10.1242/jeb.018887

Blood flow and metabolic regulation in seal muscle during apnea. / Ponganis, Paul J.; Kreutzer, Ulrike; Stockard, Torre K.; Lin, Ping Chang; Sailasuta, Napapon; Tran, Tuan Khan; Hurd, Ralph; Jue, Thomas.

In: Journal of Experimental Biology, Vol. 211, No. 20, 15.10.2008, p. 3323-3332.

Research output: Contribution to journalArticle

Ponganis, PJ, Kreutzer, U, Stockard, TK, Lin, PC, Sailasuta, N, Tran, TK, Hurd, R & Jue, T 2008, 'Blood flow and metabolic regulation in seal muscle during apnea', Journal of Experimental Biology, vol. 211, no. 20, pp. 3323-3332. https://doi.org/10.1242/jeb.018887
Ponganis PJ, Kreutzer U, Stockard TK, Lin PC, Sailasuta N, Tran TK et al. Blood flow and metabolic regulation in seal muscle during apnea. Journal of Experimental Biology. 2008 Oct 15;211(20):3323-3332. https://doi.org/10.1242/jeb.018887
Ponganis, Paul J. ; Kreutzer, Ulrike ; Stockard, Torre K. ; Lin, Ping Chang ; Sailasuta, Napapon ; Tran, Tuan Khan ; Hurd, Ralph ; Jue, Thomas. / Blood flow and metabolic regulation in seal muscle during apnea. In: Journal of Experimental Biology. 2008 ; Vol. 211, No. 20. pp. 3323-3332.
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