Blood copper concentrations and cuproenzyme activities in a colony of cats

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Abstract

Background - There is little published information on plasma copper and whole blood copper concentrations in cats, and except for extracellular superoxide dismutase (EC SOD), we are unaware of published values for cat cuproenzyme activities. Objective - In this study we determined plasma and whole blood copper concentrations, plasma ceruloplasmin (CP), EC SOD and diamine oxidase (DAO) activities, and erythrocyte superoxide dismutase (RBC SOD) activity in a specific pathogen-free colony of cats. Results were evaluated for differences based on age and sex. Methods - Blood was obtained from 20 adult cats (10 males, 10 females; >1 year of age) and 20 immature cats (10 males, 10 females; <1 year of age). Copper concentrations were determined using atomic absorption spectrophotometry. EC SOD and RBC SOD activities were determined using the pyrogallol oxidation method, and CP activity was determined by the oxidation of 0-dianisidine dihydrochloride. DAO activity was measured using a colorimetric assay. Differences among groups were analyzed using ANOVA. Results - There were significant differences in mean plasma copper concentration, and CP and DAO activities (P=.001, .0001, and .02, respectively) among the 4 groups of cats. No age differences were identified. Male cats had significantly greater mean (± SEM) plasma copper (15.4 ± 0.9 μmol/L versus 11.3 ± 0.6 μmol/L; P = .001) concentrations, and CP (66.8 ± 2.9 U/L versus 39.7 ± 2.1 U/L; P=.0001) and DAO (6.68 ± 0.16 U/L versus 6.15 ± 0.1 U/L; P = .03) activities compared with female cats. Males had significantly greater whole blood copper concentrations (16.16 ± 0.55 μmol/L versus 13.36 ± 0.52 μmol/L; P = .002) than female cats. Conclusion - Differences exist between male and female cats with respect to blood copper concentrations, and CP and DAO activities.

Original languageEnglish (US)
Pages (from-to)183-188
Number of pages6
JournalVeterinary Clinical Pathology
Volume31
Issue number4
StatePublished - Dec 2002

Fingerprint

Copper
Amine Oxidase (Copper-Containing)
Cats
Blood
copper
Ceruloplasmin
cats
ferroxidase
blood
superoxide dismutase
Superoxide Dismutase
Plasmas
erythrocytes
Dianisidine
Pyrogallol
Oxidation
oxidation
Spectrophotometry
Pathogens
Specific Pathogen-Free Organisms

Keywords

  • Cat
  • Ceruloplasmin
  • Copper
  • Diamine oxidase
  • Superoxide dismutase

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

Cite this

@article{7c5a79f18bfc4fe080178defa8af983c,
title = "Blood copper concentrations and cuproenzyme activities in a colony of cats",
abstract = "Background - There is little published information on plasma copper and whole blood copper concentrations in cats, and except for extracellular superoxide dismutase (EC SOD), we are unaware of published values for cat cuproenzyme activities. Objective - In this study we determined plasma and whole blood copper concentrations, plasma ceruloplasmin (CP), EC SOD and diamine oxidase (DAO) activities, and erythrocyte superoxide dismutase (RBC SOD) activity in a specific pathogen-free colony of cats. Results were evaluated for differences based on age and sex. Methods - Blood was obtained from 20 adult cats (10 males, 10 females; >1 year of age) and 20 immature cats (10 males, 10 females; <1 year of age). Copper concentrations were determined using atomic absorption spectrophotometry. EC SOD and RBC SOD activities were determined using the pyrogallol oxidation method, and CP activity was determined by the oxidation of 0-dianisidine dihydrochloride. DAO activity was measured using a colorimetric assay. Differences among groups were analyzed using ANOVA. Results - There were significant differences in mean plasma copper concentration, and CP and DAO activities (P=.001, .0001, and .02, respectively) among the 4 groups of cats. No age differences were identified. Male cats had significantly greater mean (± SEM) plasma copper (15.4 ± 0.9 μmol/L versus 11.3 ± 0.6 μmol/L; P = .001) concentrations, and CP (66.8 ± 2.9 U/L versus 39.7 ± 2.1 U/L; P=.0001) and DAO (6.68 ± 0.16 U/L versus 6.15 ± 0.1 U/L; P = .03) activities compared with female cats. Males had significantly greater whole blood copper concentrations (16.16 ± 0.55 μmol/L versus 13.36 ± 0.52 μmol/L; P = .002) than female cats. Conclusion - Differences exist between male and female cats with respect to blood copper concentrations, and CP and DAO activities.",
keywords = "Cat, Ceruloplasmin, Copper, Diamine oxidase, Superoxide dismutase",
author = "Fascetti, {Andrea J} and Quinton Rogers and James Morris",
year = "2002",
month = "12",
language = "English (US)",
volume = "31",
pages = "183--188",
journal = "Veterinary Clinical Pathology",
issn = "0275-6382",
publisher = "American Society for Veterinary Clinical Pathology",
number = "4",

}

TY - JOUR

T1 - Blood copper concentrations and cuproenzyme activities in a colony of cats

AU - Fascetti, Andrea J

AU - Rogers, Quinton

AU - Morris, James

PY - 2002/12

Y1 - 2002/12

N2 - Background - There is little published information on plasma copper and whole blood copper concentrations in cats, and except for extracellular superoxide dismutase (EC SOD), we are unaware of published values for cat cuproenzyme activities. Objective - In this study we determined plasma and whole blood copper concentrations, plasma ceruloplasmin (CP), EC SOD and diamine oxidase (DAO) activities, and erythrocyte superoxide dismutase (RBC SOD) activity in a specific pathogen-free colony of cats. Results were evaluated for differences based on age and sex. Methods - Blood was obtained from 20 adult cats (10 males, 10 females; >1 year of age) and 20 immature cats (10 males, 10 females; <1 year of age). Copper concentrations were determined using atomic absorption spectrophotometry. EC SOD and RBC SOD activities were determined using the pyrogallol oxidation method, and CP activity was determined by the oxidation of 0-dianisidine dihydrochloride. DAO activity was measured using a colorimetric assay. Differences among groups were analyzed using ANOVA. Results - There were significant differences in mean plasma copper concentration, and CP and DAO activities (P=.001, .0001, and .02, respectively) among the 4 groups of cats. No age differences were identified. Male cats had significantly greater mean (± SEM) plasma copper (15.4 ± 0.9 μmol/L versus 11.3 ± 0.6 μmol/L; P = .001) concentrations, and CP (66.8 ± 2.9 U/L versus 39.7 ± 2.1 U/L; P=.0001) and DAO (6.68 ± 0.16 U/L versus 6.15 ± 0.1 U/L; P = .03) activities compared with female cats. Males had significantly greater whole blood copper concentrations (16.16 ± 0.55 μmol/L versus 13.36 ± 0.52 μmol/L; P = .002) than female cats. Conclusion - Differences exist between male and female cats with respect to blood copper concentrations, and CP and DAO activities.

AB - Background - There is little published information on plasma copper and whole blood copper concentrations in cats, and except for extracellular superoxide dismutase (EC SOD), we are unaware of published values for cat cuproenzyme activities. Objective - In this study we determined plasma and whole blood copper concentrations, plasma ceruloplasmin (CP), EC SOD and diamine oxidase (DAO) activities, and erythrocyte superoxide dismutase (RBC SOD) activity in a specific pathogen-free colony of cats. Results were evaluated for differences based on age and sex. Methods - Blood was obtained from 20 adult cats (10 males, 10 females; >1 year of age) and 20 immature cats (10 males, 10 females; <1 year of age). Copper concentrations were determined using atomic absorption spectrophotometry. EC SOD and RBC SOD activities were determined using the pyrogallol oxidation method, and CP activity was determined by the oxidation of 0-dianisidine dihydrochloride. DAO activity was measured using a colorimetric assay. Differences among groups were analyzed using ANOVA. Results - There were significant differences in mean plasma copper concentration, and CP and DAO activities (P=.001, .0001, and .02, respectively) among the 4 groups of cats. No age differences were identified. Male cats had significantly greater mean (± SEM) plasma copper (15.4 ± 0.9 μmol/L versus 11.3 ± 0.6 μmol/L; P = .001) concentrations, and CP (66.8 ± 2.9 U/L versus 39.7 ± 2.1 U/L; P=.0001) and DAO (6.68 ± 0.16 U/L versus 6.15 ± 0.1 U/L; P = .03) activities compared with female cats. Males had significantly greater whole blood copper concentrations (16.16 ± 0.55 μmol/L versus 13.36 ± 0.52 μmol/L; P = .002) than female cats. Conclusion - Differences exist between male and female cats with respect to blood copper concentrations, and CP and DAO activities.

KW - Cat

KW - Ceruloplasmin

KW - Copper

KW - Diamine oxidase

KW - Superoxide dismutase

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M3 - Article

VL - 31

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