Blimp-1-dependent and -independent natural antibody production by B-1 and B-1-derived plasma cells

Hannah P. Savage, Vanessa M. Yenson, Sanjam S. Sawhney, Betty J. Mousseau, Frances E. Lund, Nicole Baumgarth

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Natural antibodies contribute to tissue homeostasis and protect against infections. They are secreted constitutively without external antigenic stimulation. The differentiation state and regulatory pathways that enable continuous natural antibody production by B-1 cells, the main cellular source in mice, remain incompletely understood. Here we demonstrate that natural IgM-secreting B-1 cells in the spleen and bone marrow are heterogeneous, consisting of (a) terminally differentiated B-1-derived plasma cells expressing the transcriptional regulator of differentiation, Blimp-1, (b) Blimp-1+, and (c) Blimp-1neg phenotypic B-1 cells. Blimp-1neg IgM-secreting B-1 cells are not simply intermediates of cellular differentiation. Instead, they secrete similar amounts of IgM in wild-type and Blimp-1-deficient (PRDM-1ΔEx1A) mice. Blimp-1neg B-1 cells are also a major source of IgG3. Consequently, deletion of Blimp-1 changes neither serum IgG3 levels nor the amount of IgG3 secreted per cell. Thus, the pool of natural antibody-secreting B-1 cells is heterogeneous and contains a distinct subset of cells that do not use Blimp-1 for initiation or maximal antibody secretion.

Original languageEnglish (US)
Pages (from-to)2777-2794
Number of pages18
JournalJournal of Experimental Medicine
Volume214
Issue number9
DOIs
StatePublished - 2017

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Plasma Cells
Antibody Formation
Immunoglobulin M
Immunoglobulin G
Antibodies
Homeostasis
Spleen
Bone Marrow
Infection
Serum

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Blimp-1-dependent and -independent natural antibody production by B-1 and B-1-derived plasma cells. / Savage, Hannah P.; Yenson, Vanessa M.; Sawhney, Sanjam S.; Mousseau, Betty J.; Lund, Frances E.; Baumgarth, Nicole.

In: Journal of Experimental Medicine, Vol. 214, No. 9, 2017, p. 2777-2794.

Research output: Contribution to journalArticle

Savage, Hannah P. ; Yenson, Vanessa M. ; Sawhney, Sanjam S. ; Mousseau, Betty J. ; Lund, Frances E. ; Baumgarth, Nicole. / Blimp-1-dependent and -independent natural antibody production by B-1 and B-1-derived plasma cells. In: Journal of Experimental Medicine. 2017 ; Vol. 214, No. 9. pp. 2777-2794.
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